| IED ID | IndEnz0005000366 |
| Enzyme Type ID | lipase000366 |
| Protein Name |
N-acylneuraminate cytidylyltransferase EC 2.7.7.43 CMP-N-acetylneuraminic acid synthase CMP-NeuNAc synthase |
| Gene Name | neuA |
| Organism | Escherichia coli |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli |
| Enzyme Sequence | MRTKIIAIIPARSGSKGLRNKNALMLIDKPLLAYTIEAALQSEMFEKVIVTTDSEQYGAIAESYGADFLLRPEELATDKASSFEFIKHALSIYTDYESFALLQPTSPFRDSTHIIEAVKLYQTLEKYQCVVSVTRSNKPSQIIRPLDDYSTLSFFDLDYSKYNRNSIVEYHPNGAIFIANKQHYLHTKHFFGRYSLAYIMDKESSLDIDDRMDFELAITIQQKKNRQKIDLYQNIHNRINEKRNEFDSVSDITLIGHSLFDYWDVKKINDIEVNNLGIAGINSKEYYEYIIEKELIVNFGEFVFIFFGTNDIVVSDWKKEDTLWYLKKTCQYIKKKNAASKIYLLSVPPVFGRIDRDNRIINDLNSYLRENVDFAKFISLDHVLKDSYGNLNKMYTYDGLHFNSNGYTVLENEIAEIVK |
| Enzyme Length | 419 |
| Uniprot Accession Number | P13266 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by the CTP analogs 5-mercuri-CTP and CTP-2',3'-dialdehyde. {ECO:0000269|PubMed:2826425}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=an N-acylneuraminate + CTP = a CMP-N-acyl-beta-neuraminate + diphosphate; Xref=Rhea:RHEA:11344, ChEBI:CHEBI:33019, ChEBI:CHEBI:37563, ChEBI:CHEBI:60073, ChEBI:CHEBI:68671; EC=2.7.7.43; Evidence={ECO:0000269|PubMed:2826425}; |
| DNA Binding | |
| EC Number | 2.7.7.43 |
| Enzyme Function | FUNCTION: Catalyzes the formation of CMP-N-acetylneuraminic acid (CMP-NeuNAc), which is essential for the formation of the capsule. {ECO:0000269|PubMed:2826425}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 9.0-10.0. {ECO:0000269|PubMed:2826425}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1) |
| Keywords | Capsule biogenesis/degradation;Cytoplasm;Direct protein sequencing;Magnesium;Manganese;Nucleotidyltransferase;Transferase |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:2826425}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 48,736 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=4 mM for NeuNAc (at pH 9.0) {ECO:0000269|PubMed:2826425}; KM=0.31 mM for CTP (at pH 9.0) {ECO:0000269|PubMed:2826425}; |
| Metal Binding | |
| Rhea ID | RHEA:11344 |
| Cross Reference Brenda |