| IED ID | IndEnz0005000370 |
| Enzyme Type ID | lipase000370 |
| Protein Name |
Acetylxylan esterase EC 3.1.1.72 |
| Gene Name | axeA1 PRU_2212 |
| Organism | Prevotella ruminicola (strain ATCC 19189 / JCM 8958 / 23) |
| Taxonomic Lineage | cellular organisms Bacteria FCB group Bacteroidetes/Chlorobi group Bacteroidetes Bacteroidia Bacteroidales Prevotellaceae Prevotella Prevotella ruminicola (Bacteroides ruminicola) Prevotella ruminicola (strain ATCC 19189 / JCM 8958 / 23) |
| Enzyme Sequence | MNRKLFMTGLLMLAMTMQAQTAKKFTLNLSDDGKAQMVCFLPENPSGRAIVGVPGGGYSMLSNTHEGYQASDWLNKQGIAYFVVNYRLPHGDRTIPVGDVEQGFRIVRDSAKVWNINPNDVGIMGFSAGGHLSSVISTMSPYEVRPNFSILFYPVISMDERVSHKWSCINFLGKEGYKDPKLIGQYSTQNAVRSHLTPPACIISANDDRLVPVVTNGIQYYSAMRNAGNECSLFIYPSGDHGFGFGTWFKYHDQLLQDLGNWLKSIPAPKEDAIRVACIGNSITDGFGIDMRAKYGYPAQLQGILGDGYWVKNFGVSARTMLNKGDFPYMNEMAWKDALAFKPDVVVIKLGTNDSKPENWQYGSEFRQDLEQMIKALRPDLAQPAKKGKKKAKAAAQPAGPKILLCTPIPAFKPSWNINDKVITDEIIPIQQEVAKQYGLQIIDLHALMLNDGDKVVDDGIHPNEKGAKKMAEIIAAAIK |
| Enzyme Length | 480 |
| Uniprot Accession Number | D5EV35 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Deacetylation of xylans and xylo-oligosaccharides.; EC=3.1.1.72; Evidence={ECO:0000269|PubMed:21742923}; |
| DNA Binding | |
| EC Number | 3.1.1.72 |
| Enzyme Function | FUNCTION: Involved in degradation of plant cell wall polysaccharides. Is an acetyl esterase with broad substrate specificity, releasing acetic acid from acetylated xylo-oligosaccharides and acetylated xylan as well as xylose-tetraacetate, 4-O-methylumbelliferyl acetate, glucose-pentaacetate, and cephalosporin C. Appears to have greater activity on oligosaccharides than on polymeric substrates. Is also able to release acetic acid from xylo-oligosaccharides with 4-O-methylglucuronic acid side groups proximally located to O-acetyl esters. Preferentially targets xylo-oligosaccharides possessing three or more O-acetyl groups, but following their depletion it is active on the less acetylated portion of the substrate. {ECO:0000269|PubMed:21742923}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 30 to 35 degrees Celsius with glucose-pentaacetate as substrate. Active from 20 to 50 degrees Celsius. Still exhibits 40 to 70% of the maximum activity after 20 hours of incubation at 50 degrees Celsius. {ECO:0000269|PubMed:21742923}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.0 with glucose-pentaacetate as substrate. Active from pH 5.0 to 8.0. {ECO:0000269|PubMed:21742923}; |
| Pathway | PATHWAY: Glycan degradation; xylan degradation. {ECO:0000269|PubMed:21742923}. |
| nucleotide Binding | |
| Features | Chain (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Hydrolase;Polysaccharide degradation;Reference proteome;Signal;Xylan degradation |
| Interact With | |
| Induction | INDUCTION: Constitutively expressed. {ECO:0000269|PubMed:21742923}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 53,109 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |