| IED ID |
IndEnz0005000373 |
| Enzyme Type ID |
lipase000373 |
| Protein Name |
Crystal protein
|
| Gene Name |
cryS DDB_G0285419 |
| Organism |
Dictyostelium discoideum (Slime mold) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Amoebozoa
Evosea
Eumycetozoa
Dictyostelia (dictyostelid cellular slime molds)
Dictyosteliales
Dictyosteliaceae
Dictyostelium
Dictyostelium discoideum (Slime mold)
|
| Enzyme Sequence |
MNKIIILLIILLSFDIISAAKKFGRKGIRTLGDNEVLLSDGAIRGTVTDTHRVFYGIPFARPPIDELRYEDPQPPKPWSYVRDGTKQRDQCIQDCKLGKGSCSEVGTSEDCLYLDVFIPRTVNPGSKVPVMVFIPGGAFTQGTGSCPLYDGLKFANSSVIVVNVNYRLGVLGFLCTGLLSGNFGFLDQVMALDWVQENIEVFGGDKNQVTIYGESAGAFSVAAHLSSEKSEGKFHRAILSSTPYTVGLKSQTVARGFAGRFSSKIGCDLEDIDCHRSKSPEEILAIQKELGLAIGDKILDAFTIWSPVVDGINVNEQPLTMIKQGTTHDVPTIIGDNQDEAILFVYMTYKNVVIPSSYRTMVHVLFGIANGNKVLEHYPLPGFLKDSRPILSKLLTDYLFRCPGRYHVSKSAQANESPIYHYQYKQVLSGGHSFEACEGLVCHGTELPMVFNTYESALDLDLEEEEEEFAEQLNNYFVNFIKYSNPSHPNGLPTPKVWNPTTKTTNTSLVMKLGFEVKDLITNDPKCDLFDSLSYNGYTKDQNRMRKSKK |
| Enzyme Length |
550 |
| Uniprot Accession Number |
P21837 |
| Absorption |
|
| Active Site |
ACT_SITE 215; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 340; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 443; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
|
| EC Number |
|
| Enzyme Function |
|
| Temperature Dependency |
|
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Active site (3); Chain (1); Disulfide bond (2); Glycosylation (2); Signal peptide (1) |
| Keywords |
Cytoplasmic vesicle;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Reference proteome;Serine esterase;Signal |
| Interact With |
|
| Induction |
|
| Subcellular Location |
SUBCELLULAR LOCATION: Cytoplasmic vesicle, esterosome membrane. Note=Membrane-enclosed crystals (esterosome). |
| Modified Residue |
|
| Post Translational Modification |
|
| Signal Peptide |
SIGNAL 1..19; /evidence=ECO:0000269|PubMed:2307702 |
| Structure 3D |
|
| Cross Reference PDB |
- |
| Mapped Pubmed ID |
14504294;
|
| Motif |
|
| Gene Encoded By |
|
| Mass |
61,066 |
| Kinetics |
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| Metal Binding |
|
| Rhea ID |
|
| Cross Reference Brenda |
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