| IED ID | IndEnz0005000388 |
| Enzyme Type ID | lipase000388 |
| Protein Name |
B-cell linker protein B-cell adapter SH2 domain-containing protein Cytoplasmic adapter protein Src homology 2 domain-containing leukocyte protein of 65 kDa SLP-65 |
| Gene Name | BLNK BASH |
| Organism | Gallus gallus (Chicken) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Archelosauria Archosauria Dinosauria Saurischia Theropoda Coelurosauria Aves Neognathae Galloanserae Galliformes Phasianidae (turkeys) Phasianinae Gallus Gallus gallus (Chicken) |
| Enzyme Sequence | MDKLNKLAVPAGEKFRKLQKMVHDIKKNESGIINKFKKFQNEQVALICKTGKDTWDRLKKKPPPSLPRRDYASEHADNEEEQWSDDFDSDYENPDGHSDSEMYVVPSEENPDDSYEPPPSEQEKKKIPSSFPISRGEYADNRTSHHQLPPINKPLPSTPSSALPRPKKPSLPSPAAKPKLPLKPRECSDDEDNYIVPVDNDDDNYIEPTESSTPPPAKPPVNRFMKPPAKSALPTPPKPSLASDMQEVYEVPEEEEELSPPPVTRFTKPLPATRAQNAEHSHMHSMTRESPKLDASRNILPLPRNRLHPKTDHEANNNDENHSFSNTQESKFPPGAAPSPLPRALKKTSNAVNPAKPCLPSRDTFTVNEDKPTAADRRRGSSHEFPLPPLPSGTPKSSLQKPLVLPKVPEAPSRALGTSPHSSISSISSTADQDAGVHSKAWYAATCDRKTAEDALYRSNKDGSFLIRKSSGQDSRQPYTLVVFYNRRVYNIPIRFIESTRQYALGREKCGEERFDSVAEIVENHQHTSLVLIDSQNNTKDSTKLKYIVRVS |
| Enzyme Length | 552 |
| Uniprot Accession Number | Q9YGC1 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition (By similarity). Plays a critical role in B-cell development in the bursa. Plays an important role in BCR-induced apoptosis. {ECO:0000250, ECO:0000269|PubMed:10023776, ECO:0000269|PubMed:16912232, ECO:0000269|PubMed:17681949, ECO:0000269|PubMed:9834055}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (1); Chain (1); Compositional bias (4); Domain (1); Modified residue (6); Mutagenesis (7); Region (1) |
| Keywords | Alternative splicing;B-cell activation;Cell membrane;Cytoplasm;Membrane;Phosphoprotein;Reference proteome;SH2 domain |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:17681949}. Cell membrane {ECO:0000269|PubMed:17681949}. Note=BCR activation results in the translocation to membrane fraction. |
| Modified Residue | MOD_RES 91; /note=Phosphotyrosine; by SYK; /evidence=ECO:0000269|PubMed:12456653; MOD_RES 103; /note=Phosphotyrosine; by SYK; /evidence=ECO:0000269|PubMed:12456653; MOD_RES 115; /note=Phosphotyrosine; by SYK; /evidence=ECO:0000269|PubMed:12456653; MOD_RES 194; /note=Phosphotyrosine; by SYK; /evidence=ECO:0000269|PubMed:12456653; MOD_RES 205; /note=Phosphotyrosine; by SYK; /evidence=ECO:0000269|PubMed:12456653; MOD_RES 249; /note=Phosphotyrosine; by SYK; /evidence=ECO:0000269|PubMed:12456653 |
| Post Translational Modification | PTM: Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways (By similarity). Phosphorylation of Tyr-103, Tyr-194 and Tyr-205 facilitates PLCG1 binding. Phosphorylation of Tyr-115 facilitates BTK binding. Phosphorylation of Tyr-91 facilitates VAV1 and NCK1 binding. {ECO:0000250, ECO:0000269|PubMed:12456653, ECO:0000269|PubMed:9834055}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 61,823 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |