| IED ID | IndEnz0005000393 |
| Enzyme Type ID | lipase000393 |
| Protein Name |
Carboxylesterase Culp6 homolog EC 3.1.1.- |
| Gene Name | MSMEG_6394 MSMEI_6226 |
| Organism | Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycolicibacterium Mycolicibacterium smegmatis (Mycobacterium smegmatis) Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) |
| Enzyme Sequence | MAKNARRKRHRILALIAAAAMALVVVLVVTIVVVIMRRPDTPATPPPSAEPPGGVVVPPGTRKPRPEFQSADCPDVMMVSIPGTWESSPTDDPFNPTQFPLSLMSNISKPLAEQFGPDRLQVYTTPYTAQFHNPFAADKQMSYNDSRAEGMRTTVKAMTDMNDRCPLTSYVIAGFSQGAVIAGDIASDIGNGRGPVDEDLVLGVTLIADGRRQMGVGQDVGPNPAGQGAEITLHEVPALSALGLTMTGPRPGGFGALDNRTNQICGSGDLICSAPEQAFSVFNLPKTLETLSGSAAGPVHALYNTPQFWVENGQTATQWTLEWARNLVENAPHPKHG |
| Enzyme Length | 337 |
| Uniprot Accession Number | A0R619 |
| Absorption | |
| Active Site | ACT_SITE 176; /evidence=ECO:0000305|PubMed:20656688; ACT_SITE 269; /evidence=ECO:0000305|PubMed:20656688; ACT_SITE 300; /evidence=ECO:0000305|PubMed:20656688 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by tetrahydrolipstatin (THL), a specific lipase inhibitor. {ECO:0000269|PubMed:20656688}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a butanoate ester + H2O = an aliphatic alcohol + butanoate + H(+); Xref=Rhea:RHEA:47348, ChEBI:CHEBI:2571, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17968, ChEBI:CHEBI:50477; Evidence={ECO:0000269|PubMed:20656688}; |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Esterase that may be involved in cell wall biosynthesis and/or maintenance. Hydrolyzes pNP-butyrate (C4). {ECO:0000269|PubMed:20656688}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (9); Chain (1); Compositional bias (1); Disulfide bond (2); Helix (10); Region (1); Transmembrane (1); Turn (6) |
| Keywords | 3D-structure;Cell membrane;Disulfide bond;Hydrolase;Membrane;Reference proteome;Serine esterase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 3AJA; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 35,888 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=5.22 mM for pNP-butyrate {ECO:0000269|PubMed:20656688}; Vmax=783 nmol/min/mg enzyme with pNP-butyrate as substrate {ECO:0000269|PubMed:20656688}; Note=kcat is 0.448 sec(-1) with pNP-butyrate as substrate. {ECO:0000269|PubMed:20656688}; |
| Metal Binding | |
| Rhea ID | RHEA:47348 |
| Cross Reference Brenda |