IED Industry Enzyme Database

Detail Information for IndEnz0005000430
IED ID IndEnz0005000430
Enzyme Type ID lipase000430
Protein Name Fatty-acid amide hydrolase 1
EC 3.5.1.99
Anandamide amidohydrolase 1
Fatty acid ester hydrolase
EC 3.1.1.-
Oleamide hydrolase 1
Gene Name Faah Faah1
Organism Mus musculus (Mouse)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse)
Enzyme Sequence MVLSEVWTALSGLSGVCLACSLLSAAVVLRWTRSQTARGAVTRARQKQRAGLETMDKAVQRFRLQNPDLDSEALLALPLLQLVQKLQSGELSPEAVLFTYLGKAWEVNKGTNCVTSYLTDCETQLSQAPRQGLLYGVPVSLKECFSYKGHASTLGLSLNEGVTSESDCVVVQVLKLQGAVPFVHTNVPQSMLSYDCSNPLFGQTMNPWKPSKSPGGSSGGEGALIGSGGSPLGLGTDIGGSIRFPSAFCGICGLKPTGNRLSKSGLKSCVYGQTAVQLSVGPMARDVDSLALCMKALLCEDLFRLDSTIPPLPFREEIYRSSRPLRVGYYETDNYTMPTPAMRRAVMETKQSLEAAGHTLVPFLPNNIPYALEVLSAGGLFSDGGCSFLQNFKGDFVDPCLGDLVLVLKLPRWFKKLLSFLLKPLFPRLAAFLNSMCPRSAEKLWELQHEIEMYRQSVIAQWKAMNLDVVLTPMLGPALDLNTPGRATGAISYTVLYNCLDFPAGVVPVTTVTAEDDAQMEHYKGYFGDMWDNILKKGMKKGIGLPVAVQCVALPWQEELCLRFMREVERLMTPEKRPS
Enzyme Length 579
Uniprot Accession Number O08914
Absorption
Active Site ACT_SITE 142; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 217; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 241; /note=Acyl-ester intermediate; /evidence=ECO:0000250
Activity Regulation ACTIVITY REGULATION: Inhibited the trifluoromethyl compound PF-3845. {ECO:0000269|PubMed:32271712}.
Binding Site BINDING 191; /note=Substrate; via carbonyl oxygen; /evidence=ECO:0000250; BINDING 217; /note=Substrate; /evidence=ECO:0000250
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=H2O + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine = (5Z,8Z,11Z,14Z)-eicosatetraenoate + ethanolamine; Xref=Rhea:RHEA:26136, ChEBI:CHEBI:2700, ChEBI:CHEBI:15377, ChEBI:CHEBI:32395, ChEBI:CHEBI:57603; EC=3.5.1.99; Evidence={ECO:0000269|PubMed:15533037, ECO:0000269|PubMed:32271712};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:26137; Evidence={ECO:0000269|PubMed:15533037, ECO:0000269|PubMed:32271712}; CATALYTIC ACTIVITY: Reaction=(9Z)-octadecenamide + H2O = (9Z)-octadecenoate + NH4(+); Xref=Rhea:RHEA:26506, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:30823, ChEBI:CHEBI:116314; EC=3.5.1.99; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:26507; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycerol + H(+); Xref=Rhea:RHEA:26132, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:32395, ChEBI:CHEBI:52392; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:26133; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=H2O + N-(9Z-hexadecenoyl) ethanolamine = (9Z)-hexadecenoate + ethanolamine; Xref=Rhea:RHEA:35563, ChEBI:CHEBI:15377, ChEBI:CHEBI:32372, ChEBI:CHEBI:57603, ChEBI:CHEBI:71465; Evidence={ECO:0000269|PubMed:15533037};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35564; Evidence={ECO:0000269|PubMed:15533037}; CATALYTIC ACTIVITY: Reaction=H2O + N-(9Z-octadecenoyl) ethanolamine = (9Z)-octadecenoate + ethanolamine; Xref=Rhea:RHEA:45060, ChEBI:CHEBI:15377, ChEBI:CHEBI:30823, ChEBI:CHEBI:57603, ChEBI:CHEBI:71466; Evidence={ECO:0000269|PubMed:15533037};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45061; Evidence={ECO:0000269|PubMed:15533037}; CATALYTIC ACTIVITY: Reaction=H2O + N-octadecanoyl ethanolamine = ethanolamine + octadecanoate; Xref=Rhea:RHEA:63124, ChEBI:CHEBI:15377, ChEBI:CHEBI:25629, ChEBI:CHEBI:57603, ChEBI:CHEBI:85299; Evidence={ECO:0000269|PubMed:15533037};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63125; Evidence={ECO:0000269|PubMed:15533037}; CATALYTIC ACTIVITY: Reaction=H2O + N-docosanoyl-ethanolamine = docosanoate + ethanolamine; Xref=Rhea:RHEA:63128, ChEBI:CHEBI:15377, ChEBI:CHEBI:23858, ChEBI:CHEBI:57603, ChEBI:CHEBI:146186; Evidence={ECO:0000269|PubMed:15533037};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63129; Evidence={ECO:0000269|PubMed:15533037}; CATALYTIC ACTIVITY: Reaction=H2O + N-tetracosanoyl-taurine = taurine + tetracosanoate; Xref=Rhea:RHEA:63140, ChEBI:CHEBI:15377, ChEBI:CHEBI:31014, ChEBI:CHEBI:132049, ChEBI:CHEBI:507393; Evidence={ECO:0000269|PubMed:15533037};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63141; Evidence={ECO:0000269|PubMed:15533037}; CATALYTIC ACTIVITY: Reaction=H2O + N-(15Z-tetracosenoyl)-ethanolamine = (15Z)-tetracosenoate + ethanolamine; Xref=Rhea:RHEA:63144, ChEBI:CHEBI:15377, ChEBI:CHEBI:32392, ChEBI:CHEBI:57603, ChEBI:CHEBI:146187; Evidence={ECO:0000305|PubMed:15533037};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63145; Evidence={ECO:0000305|PubMed:15533037}; CATALYTIC ACTIVITY: Reaction=H2O + N-(9Z-octadecenoyl)-taurine = (9Z)-octadecenoate + taurine; Xref=Rhea:RHEA:63148, ChEBI:CHEBI:15377, ChEBI:CHEBI:30823, ChEBI:CHEBI:146191, ChEBI:CHEBI:507393; Evidence={ECO:0000305|PubMed:15533037};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63149; Evidence={ECO:0000305|PubMed:15533037}; CATALYTIC ACTIVITY: Reaction=H2O + N-docosanoyl-taurine = docosanoate + taurine; Xref=Rhea:RHEA:63156, ChEBI:CHEBI:15377, ChEBI:CHEBI:23858, ChEBI:CHEBI:146196, ChEBI:CHEBI:507393; Evidence={ECO:0000269|PubMed:15533037};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63157; Evidence={ECO:0000269|PubMed:15533037}; CATALYTIC ACTIVITY: Reaction=H2O + N-(15Z-tetracosenoyl)-taurine = (15Z)-tetracosenoate + taurine; Xref=Rhea:RHEA:63160, ChEBI:CHEBI:15377, ChEBI:CHEBI:32392, ChEBI:CHEBI:146198, ChEBI:CHEBI:507393; Evidence={ECO:0000305|PubMed:15533037};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63161; Evidence={ECO:0000305|PubMed:15533037}; CATALYTIC ACTIVITY: Reaction=H2O + N-tricosanoyl-taurine = taurine + tricosanoate; Xref=Rhea:RHEA:63164, ChEBI:CHEBI:15377, ChEBI:CHEBI:79007, ChEBI:CHEBI:146197, ChEBI:CHEBI:507393; Evidence={ECO:0000269|PubMed:15533037};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63165; Evidence={ECO:0000269|PubMed:15533037}; CATALYTIC ACTIVITY: Reaction=(9Z,12Z,15Z)-octadecatrienamide + H2O = (9Z,12Z,15Z)-octadecatrienoate + NH4(+); Xref=Rhea:RHEA:62976, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:32387, ChEBI:CHEBI:142684; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62977; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenamide + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + NH4(+); Xref=Rhea:RHEA:63016, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:32395, ChEBI:CHEBI:137830; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63017; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=(6Z)-octadecenamide + H2O = (6Z)-octadecenoate + NH4(+); Xref=Rhea:RHEA:63008, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:32375, ChEBI:CHEBI:146168; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63009; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=(15Z)-tetracosenamide + H2O = (15Z)-tetracosenoate + NH4(+); Xref=Rhea:RHEA:63028, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:32392, ChEBI:CHEBI:146166; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63029; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=(8Z,11Z,14Z)-eicosatrienamide + H2O = (8Z,11Z,14Z)-eicosatrienoate + NH4(+); Xref=Rhea:RHEA:62996, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:71589, ChEBI:CHEBI:146163; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62997; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=(11Z,14Z,17Z)-eicosatrienamide + H2O = (11Z,14Z,17Z)-eicosatrienoate + NH4(+); Xref=Rhea:RHEA:63000, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:77223, ChEBI:CHEBI:146164; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63001; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=(11Z,14Z)-eicosadienamide + H2O = (11Z,14Z)-eicosadienoate + NH4(+); Xref=Rhea:RHEA:63004, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:77220, ChEBI:CHEBI:146165; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63005; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=(9Z,12Z)-octadecadienamide + H2O = (9Z,12Z)-octadecadienoate + NH4(+); Xref=Rhea:RHEA:63020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:30245, ChEBI:CHEBI:82984; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63021; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=H2O + tetradecamide = NH4(+) + tetradecanoate; Xref=Rhea:RHEA:62992, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:30807, ChEBI:CHEBI:137125; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62993; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=1-O-methyl-(5Z,8Z,11Z,14Z)-eicosatetraenoate + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+) + methanol; Xref=Rhea:RHEA:63052, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:32395, ChEBI:CHEBI:78033; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63053; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=(11Z)-eicosenamide + H2O = (11Z)-eicosenoate + NH4(+); Xref=Rhea:RHEA:63120, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:32426, ChEBI:CHEBI:146167; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63121; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=(9Z)-octadecenoate + glycine = H2O + N-(9Z-octadecenoyl)glycine; Xref=Rhea:RHEA:51316, ChEBI:CHEBI:15377, ChEBI:CHEBI:30823, ChEBI:CHEBI:57305, ChEBI:CHEBI:133992; Evidence={ECO:0000269|PubMed:32271712};PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:51318; Evidence={ECO:0000269|PubMed:32271712}; CATALYTIC ACTIVITY: Reaction=H2O + N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycine; Xref=Rhea:RHEA:64108, ChEBI:CHEBI:15377, ChEBI:CHEBI:32395, ChEBI:CHEBI:57305, ChEBI:CHEBI:59002; Evidence={ECO:0000269|PubMed:32271712};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64109; Evidence={ECO:0000269|PubMed:32271712}; CATALYTIC ACTIVITY: Reaction=H2O + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-serine = (5Z,8Z,11Z,14Z)-eicosatetraenoate + L-serine; Xref=Rhea:RHEA:64116, ChEBI:CHEBI:15377, ChEBI:CHEBI:32395, ChEBI:CHEBI:33384, ChEBI:CHEBI:149697; Evidence={ECO:0000269|PubMed:32271712};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64117; Evidence={ECO:0000269|PubMed:32271712};
DNA Binding
EC Number 3.5.1.99; 3.1.1.-
Enzyme Function FUNCTION: Catalyzes the hydrolysis of endogenous amidated lipids like the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides such as the taurine-conjugated fatty acids (a structural class of central nervous system (CNS) metabolites), to their corresponding fatty acids, thereby regulating the signaling functions of these molecules (PubMed:15533037, PubMed:32271712). FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids (PubMed:32271712). It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol) (By similarity). {ECO:0000250|UniProtKB:P97612, ECO:0000269|PubMed:15533037, ECO:0000269|PubMed:32271712}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Binding site (2); Chain (1); Intramembrane (1); Modified residue (1); Region (1); Sequence conflict (2); Topological domain (2); Transmembrane (1)
Keywords Endoplasmic reticulum;Golgi apparatus;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305|PubMed:32271712}; Single-pass membrane protein {ECO:0000250|UniProtKB:P97612}. Golgi apparatus membrane {ECO:0000250|UniProtKB:P97612}; Single-pass membrane protein {ECO:0000250|UniProtKB:P97612}. Note=Seems to be associated with the endoplasmic reticulum and/or Golgi apparatus. {ECO:0000250|UniProtKB:P97612}.
Modified Residue MOD_RES 241; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:O00519
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 10208977; 10526230; 10721728; 11217851; 11470906; 12065702; 12359156; 12466851; 12736361; 12770562; 15085199; 15157693; 15209515; 15247426; 15453543; 15482903; 15525780; 15576840; 15760467; 15821037; 16143133; 16418162; 16448676; 16571781; 16605240; 16866345; 16886060; 16981687; 17047668; 17164820; 17434980; 17556587; 17709120; 17944864; 18204441; 18373655; 18501510; 18554416; 18562289; 18930149; 18987328; 19095868; 19524055; 19555737; 19918051; 20298753; 20357755; 20484813; 20571484; 20657592; 20826813; 20837524; 20862299; 21042794; 21070851; 21097653; 21267068; 21346174; 21383171; 21564090; 21665953; 21930339; 22101642; 22321194; 22442717; 22776900; 22802127; 22820645; 22833670; 22912404; 22915616; 23089640; 23175820; 23241405; 23425575; 23441874; 23479425; 23570577; 23625705; 23676498; 23806692; 23892554; 23906535; 24126189; 24167249; 24215562; 24223930; 24361246; 24571443; 24952961; 25374388; 25448494; 25534441; 25575795; 25731744; 25757568; 25917763; 26362942; 26504214; 26801558; 26900150; 27001846; 27109320; 27178246; 27412859; 27731508; 27893106; 28065729; 28488728; 28583049; 28749428; 28768662; 29228105; 29846106; 29967158; 31740614; 32095523; 34375644;
Motif
Gene Encoded By
Mass 63,221
Kinetics
Metal Binding
Rhea ID RHEA:26136; RHEA:26137; RHEA:26506; RHEA:26507; RHEA:26132; RHEA:26133; RHEA:35563; RHEA:35564; RHEA:45060; RHEA:45061; RHEA:63124; RHEA:63125; RHEA:63128; RHEA:63129; RHEA:63140; RHEA:63141; RHEA:63144; RHEA:63145; RHEA:63148; RHEA:63149; RHEA:63156; RHEA:63157; RHEA:63160; RHEA:63161; RHEA:63164; RHEA:63165; RHEA:62976; RHEA:62977; RHEA:63016; RHEA:63017; RHEA:63008; RHEA:63009; RHEA:63028; RHEA:63029; RHEA:62996; RHEA:62997; RHEA:63000; RHEA:63001; RHEA:63004; RHEA:63005; RHEA:63020; RHEA:63021; RHEA:62992; RHEA:62993; RHEA:63052; RHEA:63053; RHEA:63120; RHEA:63121; RHEA:51316; RHEA:51318; RHEA:64108; RHEA:64109; RHEA:64116; RHEA:64117
Cross Reference Brenda 3.5.1.4;3.5.1.99;