| IED ID | IndEnz0005000431 |
| Enzyme Type ID | lipase000431 |
| Protein Name |
Fatty-acid amide hydrolase 1 EC 3.5.1.99 Anandamide amidase Anandamide amidohydrolase 1 Fatty acid ester hydrolase EC 3.1.1.- Oleamide hydrolase 1 |
| Gene Name | FAAH FAAH1 |
| Organism | Sus scrofa (Pig) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Suina Suidae (pigs) Sus Sus scrofa (Pig) |
| Enzyme Sequence | MVQEELWAAFSGPSGVALACCLVAAALALRWSSRRMARGAAARARQRQQAALETMDKAAQRFRLQNPDLDSEMLLALPLPQLVQKVRSGELSPEAVLFSYLQKAWEVNRGTNCVTTYLADCEAQLCQAPGQGLLYGVPVSLKECFSCKGHDSTLGLSRNQGTPAECDCVVVQVLKLQGAVPFVHTNVPQSMFSYDCSNPLFGQTTNPWMSSKSPGGSSGGEGALIAAGGSPLGLGTDIGGSIRFPSAFCGICGIKPTGNRISKSGLKGSVYGQVAVQLSVGPMARDVESLALCLRALLCEDMFRLDPTVPPLPFNEEVYASSRPLRVGYYETDNYTMPTPAMRRALLETKRSLEAAGHTLIPFLPANIPHALEALSTGGLFSDGGKRLLQNFEGDYVDSCLGDLISILRLPKWLKGLLAFMLRPLLPRLAGFLSSLRPRSAGKLWELQHEIEMYRHSVIAQWRALDLDVVLTPMLSPALDLNAPGKATGAVSYTLLYNCLDFPAGVVPVTTVTAEDEAQMEHYKGYFGDIWDKVVQKAMKRSVGLPVAVQCVALPWQEELCLRFMREVERLMAPGRQPS |
| Enzyme Length | 579 |
| Uniprot Accession Number | Q9TUI8 |
| Absorption | |
| Active Site | ACT_SITE 142; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 217; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 241; /note=Acyl-ester intermediate; /evidence=ECO:0000250 |
| Activity Regulation | ACTIVITY REGULATION: inhibited by trifluoromethyl ketone. {ECO:0000250|UniProtKB:P97612}. |
| Binding Site | BINDING 191; /note=Substrate; via carbonyl oxygen; /evidence=ECO:0000250; BINDING 217; /note=Substrate; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine = (5Z,8Z,11Z,14Z)-eicosatetraenoate + ethanolamine; Xref=Rhea:RHEA:26136, ChEBI:CHEBI:2700, ChEBI:CHEBI:15377, ChEBI:CHEBI:32395, ChEBI:CHEBI:57603; EC=3.5.1.99; Evidence={ECO:0000269|PubMed:10526230, ECO:0000269|PubMed:12664593};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:26137; Evidence={ECO:0000269|PubMed:10526230, ECO:0000269|PubMed:12664593}; CATALYTIC ACTIVITY: Reaction=(9Z)-octadecenamide + H2O = (9Z)-octadecenoate + NH4(+); Xref=Rhea:RHEA:26506, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:30823, ChEBI:CHEBI:116314; EC=3.5.1.99; Evidence={ECO:0000269|PubMed:10526230, ECO:0000269|PubMed:12664593};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:26507; Evidence={ECO:0000269|PubMed:10526230, ECO:0000269|PubMed:12664593}; CATALYTIC ACTIVITY: Reaction=2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycerol + H(+); Xref=Rhea:RHEA:26132, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:32395, ChEBI:CHEBI:52392; Evidence={ECO:0000269|PubMed:10526230, ECO:0000269|PubMed:12664593};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:26133; Evidence={ECO:0000269|PubMed:10526230, ECO:0000269|PubMed:12664593}; CATALYTIC ACTIVITY: Reaction=1-O-methyl-(5Z,8Z,11Z,14Z)-eicosatetraenoate + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+) + methanol; Xref=Rhea:RHEA:63052, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:32395, ChEBI:CHEBI:78033; Evidence={ECO:0000269|PubMed:10526230};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63053; Evidence={ECO:0000269|PubMed:10526230}; CATALYTIC ACTIVITY: Reaction=(9Z,12Z,15Z)-octadecatrienamide + H2O = (9Z,12Z,15Z)-octadecatrienoate + NH4(+); Xref=Rhea:RHEA:62976, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:32387, ChEBI:CHEBI:142684; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62977; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenamide + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + NH4(+); Xref=Rhea:RHEA:63016, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:32395, ChEBI:CHEBI:137830; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63017; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=(6Z)-octadecenamide + H2O = (6Z)-octadecenoate + NH4(+); Xref=Rhea:RHEA:63008, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:32375, ChEBI:CHEBI:146168; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63009; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=(15Z)-tetracosenamide + H2O = (15Z)-tetracosenoate + NH4(+); Xref=Rhea:RHEA:63028, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:32392, ChEBI:CHEBI:146166; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63029; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=(8Z,11Z,14Z)-eicosatrienamide + H2O = (8Z,11Z,14Z)-eicosatrienoate + NH4(+); Xref=Rhea:RHEA:62996, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:71589, ChEBI:CHEBI:146163; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62997; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=(11Z,14Z,17Z)-eicosatrienamide + H2O = (11Z,14Z,17Z)-eicosatrienoate + NH4(+); Xref=Rhea:RHEA:63000, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:77223, ChEBI:CHEBI:146164; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63001; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=(11Z,14Z)-eicosadienamide + H2O = (11Z,14Z)-eicosadienoate + NH4(+); Xref=Rhea:RHEA:63004, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:77220, ChEBI:CHEBI:146165; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63005; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=(9Z,12Z)-octadecadienamide + H2O = (9Z,12Z)-octadecadienoate + NH4(+); Xref=Rhea:RHEA:63020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:30245, ChEBI:CHEBI:82984; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63021; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=H2O + tetradecamide = NH4(+) + tetradecanoate; Xref=Rhea:RHEA:62992, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:30807, ChEBI:CHEBI:137125; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62993; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=H2O + N-(9Z-octadecenoyl) ethanolamine = (9Z)-octadecenoate + ethanolamine; Xref=Rhea:RHEA:45060, ChEBI:CHEBI:15377, ChEBI:CHEBI:30823, ChEBI:CHEBI:57603, ChEBI:CHEBI:71466; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45061; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=H2O + N-(9Z-octadecenoyl)-taurine = (9Z)-octadecenoate + taurine; Xref=Rhea:RHEA:63148, ChEBI:CHEBI:15377, ChEBI:CHEBI:30823, ChEBI:CHEBI:146191, ChEBI:CHEBI:507393; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63149; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=(11Z)-eicosenamide + H2O = (11Z)-eicosenoate + NH4(+); Xref=Rhea:RHEA:63120, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:32426, ChEBI:CHEBI:146167; Evidence={ECO:0000250|UniProtKB:P97612};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63121; Evidence={ECO:0000250|UniProtKB:P97612}; CATALYTIC ACTIVITY: Reaction=H2O + N-(9Z-hexadecenoyl) ethanolamine = (9Z)-hexadecenoate + ethanolamine; Xref=Rhea:RHEA:35563, ChEBI:CHEBI:15377, ChEBI:CHEBI:32372, ChEBI:CHEBI:57603, ChEBI:CHEBI:71465; Evidence={ECO:0000250|UniProtKB:O08914};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35564; Evidence={ECO:0000250|UniProtKB:O08914}; CATALYTIC ACTIVITY: Reaction=H2O + N-octadecanoyl ethanolamine = ethanolamine + octadecanoate; Xref=Rhea:RHEA:63124, ChEBI:CHEBI:15377, ChEBI:CHEBI:25629, ChEBI:CHEBI:57603, ChEBI:CHEBI:85299; Evidence={ECO:0000250|UniProtKB:O08914};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63125; Evidence={ECO:0000250|UniProtKB:O08914}; CATALYTIC ACTIVITY: Reaction=H2O + N-docosanoyl-ethanolamine = docosanoate + ethanolamine; Xref=Rhea:RHEA:63128, ChEBI:CHEBI:15377, ChEBI:CHEBI:23858, ChEBI:CHEBI:57603, ChEBI:CHEBI:146186; Evidence={ECO:0000250|UniProtKB:O08914};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63129; Evidence={ECO:0000250|UniProtKB:O08914}; CATALYTIC ACTIVITY: Reaction=H2O + N-tetracosanoyl-taurine = taurine + tetracosanoate; Xref=Rhea:RHEA:63140, ChEBI:CHEBI:15377, ChEBI:CHEBI:31014, ChEBI:CHEBI:132049, ChEBI:CHEBI:507393; Evidence={ECO:0000250|UniProtKB:O08914};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63141; Evidence={ECO:0000250|UniProtKB:O08914}; CATALYTIC ACTIVITY: Reaction=H2O + N-(15Z-tetracosenoyl)-ethanolamine = (15Z)-tetracosenoate + ethanolamine; Xref=Rhea:RHEA:63144, ChEBI:CHEBI:15377, ChEBI:CHEBI:32392, ChEBI:CHEBI:57603, ChEBI:CHEBI:146187; Evidence={ECO:0000250|UniProtKB:O08914};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63145; Evidence={ECO:0000250|UniProtKB:O08914}; CATALYTIC ACTIVITY: Reaction=H2O + N-docosanoyl-taurine = docosanoate + taurine; Xref=Rhea:RHEA:63156, ChEBI:CHEBI:15377, ChEBI:CHEBI:23858, ChEBI:CHEBI:146196, ChEBI:CHEBI:507393; Evidence={ECO:0000250|UniProtKB:O08914};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63157; Evidence={ECO:0000250|UniProtKB:O08914}; CATALYTIC ACTIVITY: Reaction=H2O + N-(15Z-tetracosenoyl)-taurine = (15Z)-tetracosenoate + taurine; Xref=Rhea:RHEA:63160, ChEBI:CHEBI:15377, ChEBI:CHEBI:32392, ChEBI:CHEBI:146198, ChEBI:CHEBI:507393; Evidence={ECO:0000250|UniProtKB:O08914};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63161; Evidence={ECO:0000250|UniProtKB:O08914}; CATALYTIC ACTIVITY: Reaction=H2O + N-tricosanoyl-taurine = taurine + tricosanoate; Xref=Rhea:RHEA:63164, ChEBI:CHEBI:15377, ChEBI:CHEBI:79007, ChEBI:CHEBI:146197, ChEBI:CHEBI:507393; Evidence={ECO:0000250|UniProtKB:O08914};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63165; Evidence={ECO:0000250|UniProtKB:O08914}; CATALYTIC ACTIVITY: Reaction=(9Z)-octadecenoate + glycine = H2O + N-(9Z-octadecenoyl)glycine; Xref=Rhea:RHEA:51316, ChEBI:CHEBI:15377, ChEBI:CHEBI:30823, ChEBI:CHEBI:57305, ChEBI:CHEBI:133992; Evidence={ECO:0000250|UniProtKB:O08914};PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:51318; Evidence={ECO:0000250|UniProtKB:O08914}; CATALYTIC ACTIVITY: Reaction=H2O + N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycine; Xref=Rhea:RHEA:64108, ChEBI:CHEBI:15377, ChEBI:CHEBI:32395, ChEBI:CHEBI:57305, ChEBI:CHEBI:59002; Evidence={ECO:0000250|UniProtKB:O08914};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64109; Evidence={ECO:0000250|UniProtKB:O08914}; CATALYTIC ACTIVITY: Reaction=H2O + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-serine = (5Z,8Z,11Z,14Z)-eicosatetraenoate + L-serine; Xref=Rhea:RHEA:64116, ChEBI:CHEBI:15377, ChEBI:CHEBI:32395, ChEBI:CHEBI:33384, ChEBI:CHEBI:149697; Evidence={ECO:0000250|UniProtKB:O08914};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64117; Evidence={ECO:0000250|UniProtKB:O08914}; |
| DNA Binding | |
| EC Number | 3.5.1.99; 3.1.1.- |
| Enzyme Function | FUNCTION: Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide ((9Z)-octadecenamide), the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules (PubMed:10526230, PubMed:12664593). Also catalyzes the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol) (PubMed:10526230, PubMed:12664593). FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids (By similarity). {ECO:0000250|UniProtKB:O08914, ECO:0000269|PubMed:10526230, ECO:0000269|PubMed:12664593}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Binding site (2); Chain (1); Intramembrane (1); Modified residue (1); Mutagenesis (5); Region (1); Topological domain (2); Transmembrane (1) |
| Keywords | Endoplasmic reticulum;Golgi apparatus;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P97612}; Single-pass membrane protein {ECO:0000250|UniProtKB:P97612}. Golgi apparatus membrane {ECO:0000250|UniProtKB:P97612}; Single-pass membrane protein {ECO:0000250|UniProtKB:P97612}. Note=Seems to be associated with the endoplasmic reticulum and/or Golgi apparatus. {ECO:0000250|UniProtKB:P97612}. |
| Modified Residue | MOD_RES 241; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:O00519 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 62,857 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: Vmax=65 nmol/min/mg enzyme for the hydrolysis of oleamide ((9Z)-octadecenamide) {ECO:0000269|PubMed:10526230}; Vmax=733 nmol/min/mg enzyme for the hydrolysis of 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol) {ECO:0000269|PubMed:10526230}; Vmax=118 nmol/min/mg enzyme for the hydrolysis of methyl ester of arachidonic acid (1-O-methyl-(5Z,8Z,11Z,14Z)-eicosatetraenoate) {ECO:0000269|PubMed:10526230}; Vmax=207 nmol/min/mg enzyme for the hydrolysis of anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine) {ECO:0000269|PubMed:10526230}; |
| Metal Binding | |
| Rhea ID | RHEA:26136; RHEA:26137; RHEA:26506; RHEA:26507; RHEA:26132; RHEA:26133; RHEA:63052; RHEA:63053; RHEA:62976; RHEA:62977; RHEA:63016; RHEA:63017; RHEA:63008; RHEA:63009; RHEA:63028; RHEA:63029; RHEA:62996; RHEA:62997; RHEA:63000; RHEA:63001; RHEA:63004; RHEA:63005; RHEA:63020; RHEA:63021; RHEA:62992; RHEA:62993; RHEA:45060; RHEA:45061; RHEA:63148; RHEA:63149; RHEA:63120; RHEA:63121; RHEA:35563; RHEA:35564; RHEA:63124; RHEA:63125; RHEA:63128; RHEA:63129; RHEA:63140; RHEA:63141; RHEA:63144; RHEA:63145; RHEA:63156; RHEA:63157; RHEA:63160; RHEA:63161; RHEA:63164; RHEA:63165; RHEA:51316; RHEA:51318; RHEA:64108; RHEA:64109; RHEA:64116; RHEA:64117 |
| Cross Reference Brenda | 3.5.1.4; |