| IED ID | IndEnz0005000444 |
| Enzyme Type ID | lipase000444 |
| Protein Name |
Juvenile hormone esterase JH esterase EC 3.1.1.59 |
| Gene Name | |
| Organism | Heliothis virescens (Tobacco budworm moth) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Amphiesmenoptera Lepidoptera (butterflies and moths) Glossata Neolepidoptera Heteroneura Ditrysia Obtectomera Noctuoidea Noctuidae (owlet moths) Heliothinae Heliothis Heliothis virescens (Tobacco budworm moth) |
| Enzyme Sequence | MTSHVLALAFLLHACTALAWQETNSRSVVAHLDSGIIRGVPRSADGIKFASFLGVPYAKQPVGELRFKELEPLEPWDNILNATNEGPICFQTDVLYGRLMAASEMSEACIYANIHVPWQSLPRVRGTTPLRPILVFIHGGGFAFGSGHEDLHGPEYLVTKNVIVITFNYRLNVFGFLSMNTTKIPGNAGLRDQVTLLRWVQRNAKNFGGDPSDITIAGQSAGASAAHLLTLSKATEGLFKRAILMSGTGMSYFFTTSPLFAAYISKQLLQILGINETDPEEIHRQLIDLPAEKLNEANAVLIEQIGLTTFLPIVESPLPGVTTIIDDDPEILIAEGRGKNVPLLIGFTSSECETFRNRLLNFDLVKKIQDNPTIIIPPKLLFMTPPELLMELAKTIERKYYNGTISIDNFVKSCSDGFYEYPALKLAQKRAETGGAPLYLYRFAYEGQNSIIKKVMGLNHEGVGHIEDLTYVFKVNSMSEALHASPSENDVKMKNLMTGYFLNFIKCSQPTCEDNNSLEVWPANNGMQYEDIVSPTIIRSKEFASRQQDIIEFFDSFTSRSPLE |
| Enzyme Length | 564 |
| Uniprot Accession Number | P12992 |
| Absorption | |
| Active Site | ACT_SITE 220; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 351; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 465; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O + juvenile hormone I = H(+) + juvenile hormone I carboxylate + methanol; Xref=Rhea:RHEA:46916, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:83641, ChEBI:CHEBI:87109; EC=3.1.1.59; Evidence={ECO:0000250|UniProtKB:P19985}; CATALYTIC ACTIVITY: Reaction=H2O + juvenile hormone III = H(+) + juvenile hormone III carboxylate + methanol; Xref=Rhea:RHEA:46912, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:27493, ChEBI:CHEBI:83274; EC=3.1.1.59; Evidence={ECO:0000250|UniProtKB:P19985}; |
| DNA Binding | |
| EC Number | 3.1.1.59 |
| Enzyme Function | FUNCTION: JH esterase plays a crucial role in the decrease of JH activity in lepidopteran insects, by hydrolyzing the methyl ester of JH. It is also involved in the transport of JH. {ECO:0000250|UniProtKB:P19985}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (1); Glycosylation (4); Natural variant (2); Signal peptide (1) |
| Keywords | Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Serine esterase;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000269|PubMed:2745451 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 62,615 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:46916; RHEA:46912 |
| Cross Reference Brenda | 3.1.1.59; |