IED Industry Enzyme Database

Detail Information for IndEnz0005000450
IED ID IndEnz0005000450
Enzyme Type ID lipase000450
Protein Name Apolipoprotein B-100
Apo B-100

Cleaved into: Apolipoprotein B-48
Apo B-48
Gene Name APOB
Organism Homo sapiens (Human)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human)
Enzyme Sequence MDPPRPALLALLALPALLLLLLAGARAEEEMLENVSLVCPKDATRFKHLRKYTYNYEAESSSGVPGTADSRSATRINCKVELEVPQLCSFILKTSQCTLKEVYGFNPEGKALLKKTKNSEEFAAAMSRYELKLAIPEGKQVFLYPEKDEPTYILNIKRGIISALLVPPETEEAKQVLFLDTVYGNCSTHFTVKTRKGNVATEISTERDLGQCDRFKPIRTGISPLALIKGMTRPLSTLISSSQSCQYTLDAKRKHVAEAICKEQHLFLPFSYKNKYGMVAQVTQTLKLEDTPKINSRFFGEGTKKMGLAFESTKSTSPPKQAEAVLKTLQELKKLTISEQNIQRANLFNKLVTELRGLSDEAVTSLLPQLIEVSSPITLQALVQCGQPQCSTHILQWLKRVHANPLLIDVVTYLVALIPEPSAQQLREIFNMARDQRSRATLYALSHAVNNYHKTNPTGTQELLDIANYLMEQIQDDCTGDEDYTYLILRVIGNMGQTMEQLTPELKSSILKCVQSTKPSLMIQKAAIQALRKMEPKDKDQEVLLQTFLDDASPGDKRLAAYLMLMRSPSQADINKIVQILPWEQNEQVKNFVASHIANILNSEELDIQDLKKLVKEALKESQLPTVMDFRKFSRNYQLYKSVSLPSLDPASAKIEGNLIFDPNNYLPKESMLKTTLTAFGFASADLIEIGLEGKGFEPTLEALFGKQGFFPDSVNKALYWVNGQVPDGVSKVLVDHFGYTKDDKHEQDMVNGIMLSVEKLIKDLKSKEVPEARAYLRILGEELGFASLHDLQLLGKLLLMGARTLQGIPQMIGEVIRKGSKNDFFLHYIFMENAFELPTGAGLQLQISSSGVIAPGAKAGVKLEVANMQAELVAKPSVSVEFVTNMGIIIPDFARSGVQMNTNFFHESGLEAHVALKAGKLKFIIPSPKRPVKLLSGGNTLHLVSTTKTEVIPPLIENRQSWSVCKQVFPGLNYCTSGAYSNASSTDSASYYPLTGDTRLELELRPTGEIEQYSVSATYELQREDRALVDTLKFVTQAEGAKQTEATMTFKYNRQSMTLSSEVQIPDFDVDLGTILRVNDESTEGKTSYRLTLDIQNKKITEVALMGHLSCDTKEERKIKGVISIPRLQAEARSEILAHWSPAKLLLQMDSSATAYGSTVSKRVAWHYDEEKIEFEWNTGTNVDTKKMTSNFPVDLSDYPKSLHMYANRLLDHRVPQTDMTFRHVGSKLIVAMSSWLQKASGSLPYTQTLQDHLNSLKEFNLQNMGLPDFHIPENLFLKSDGRVKYTLNKNSLKIEIPLPFGGKSSRDLKMLETVRTPALHFKSVGFHLPSREFQVPTFTIPKLYQLQVPLLGVLDLSTNVYSNLYNWSASYSGGNTSTDHFSLRARYHMKADSVVDLLSYNVQGSGETTYDHKNTFTLSYDGSLRHKFLDSNIKFSHVEKLGNNPVSKGLLIFDASSSWGPQMSASVHLDSKKKQHLFVKEVKIDGQFRVSSFYAKGTYGLSCQRDPNTGRLNGESNLRFNSSYLQGTNQITGRYEDGTLSLTSTSDLQSGIIKNTASLKYENYELTLKSDTNGKYKNFATSNKMDMTFSKQNALLRSEYQADYESLRFFSLLSGSLNSHGLELNADILGTDKINSGAHKATLRIGQDGISTSATTNLKCSLLVLENELNAELGLSGASMKLTTNGRFREHNAKFSLDGKAALTELSLGSAYQAMILGVDSKNIFNFKVSQEGLKLSNDMMGSYAEMKFDHTNSLNIAGLSLDFSSKLDNIYSSDKFYKQTVNLQLQPYSLVTTLNSDLKYNALDLTNNGKLRLEPLKLHVAGNLKGAYQNNEIKHIYAISSAALSASYKADTVAKVQGVEFSHRLNTDIAGLASAIDMSTNYNSDSLHFSNVFRSVMAPFTMTIDAHTNGNGKLALWGEHTGQLYSKFLLKAEPLAFTFSHDYKGSTSHHLVSRKSISAALEHKVSALLTPAEQTGTWKLKTQFNNNEYSQDLDAYNTKDKIGVELTGRTLADLTLLDSPIKVPLLLSEPINIIDALEMRDAVEKPQEFTIVAFVKYDKNQDVHSINLPFFETLQEYFERNRQTIIVVLENVQRNLKHINIDQFVRKYRAALGKLPQQANDYLNSFNWERQVSHAKEKLTALTKKYRITENDIQIALDDAKINFNEKLSQLQTYMIQFDQYIKDSYDLHDLKIAIANIIDEIIEKLKSLDEHYHIRVNLVKTIHDLHLFIENIDFNKSGSSTASWIQNVDTKYQIRIQIQEKLQQLKRHIQNIDIQHLAGKLKQHIEAIDVRVLLDQLGTTISFERINDILEHVKHFVINLIGDFEVAEKINAFRAKVHELIERYEVDQQIQVLMDKLVELAHQYKLKETIQKLSNVLQQVKIKDYFEKLVGFIDDAVKKLNELSFKTFIEDVNKFLDMLIKKLKSFDYHQFVDETNDKIREVTQRLNGEIQALELPQKAEALKLFLEETKATVAVYLESLQDTKITLIINWLQEALSSASLAHMKAKFRETLEDTRDRMYQMDIQQELQRYLSLVGQVYSTLVTYISDWWTLAAKNLTDFAEQYSIQDWAKRMKALVEQGFTVPEIKTILGTMPAFEVSLQALQKATFQTPDFIVPLTDLRIPSVQINFKDLKNIKIPSRFSTPEFTILNTFHIPSFTIDFVEMKVKIIRTIDQMLNSELQWPVPDIYLRDLKVEDIPLARITLPDFRLPEIAIPEFIIPTLNLNDFQVPDLHIPEFQLPHISHTIEVPTFGKLYSILKIQSPLFTLDANADIGNGTTSANEAGIAASITAKGESKLEVLNFDFQANAQLSNPKINPLALKESVKFSSKYLRTEHGSEMLFFGNAIEGKSNTVASLHTEKNTLELSNGVIVKINNQLTLDSNTKYFHKLNIPKLDFSSQADLRNEIKTLLKAGHIAWTSSGKGSWKWACPRFSDEGTHESQISFTIEGPLTSFGLSNKINSKHLRVNQNLVYESGSLNFSKLEIQSQVDSQHVGHSVLTAKGMALFGEGKAEFTGRHDAHLNGKVIGTLKNSLFFSAQPFEITASTNNEGNLKVRFPLRLTGKIDFLNNYALFLSPSAQQASWQVSARFNQYKYNQNFSAGNNENIMEAHVGINGEANLDFLNIPLTIPEMRLPYTIITTPPLKDFSLWEKTGLKEFLKTTKQSFDLSVKAQYKKNKHRHSITNPLAVLCEFISQSIKSFDRHFEKNRNNALDFVTKSYNETKIKFDKYKAEKSHDELPRTFQIPGYTVPVVNVEVSPFTIEMSAFGYVFPKAVSMPSFSILGSDVRVPSYTLILPSLELPVLHVPRNLKLSLPDFKELCTISHIFIPAMGNITYDFSFKSSVITLNTNAELFNQSDIVAHLLSSSSSVIDALQYKLEGTTRLTRKRGLKLATALSLSNKFVEGSHNSTVSLTTKNMEVSVATTTKAQIPILRMNFKQELNGNTKSKPTVSSSMEFKYDFNSSMLYSTAKGAVDHKLSLESLTSYFSIESSTKGDVKGSVLSREYSGTIASEANTYLNSKSTRSSVKLQGTSKIDDIWNLEVKENFAGEATLQRIYSLWEHSTKNHLQLEGLFFTNGEHTSKATLELSPWQMSALVQVHASQPSSFHDFPDLGQEVALNANTKNQKIRWKNEVRIHSGSFQSQVELSNDQEKAHLDIAGSLEGHLRFLKNIILPVYDKSLWDFLKLDVTTSIGRRQHLRVSTAFVYTKNPNGYSFSIPVKVLADKFIIPGLKLNDLNSVLVMPTFHVPFTDLQVPSCKLDFREIQIYKKLRTSSFALNLPTLPEVKFPEVDVLTKYSQPEDSLIPFFEITVPESQLTVSQFTLPKSVSDGIAALDLNAVANKIADFELPTIIVPEQTIEIPSIKFSVPAGIVIPSFQALTARFEVDSPVYNATWSASLKNKADYVETVLDSTCSSTVQFLEYELNVLGTHKIEDGTLASKTKGTFAHRDFSAEYEEDGKYEGLQEWEGKAHLNIKSPAFTDLHLRYQKDKKGISTSAASPAVGTVGMDMDEDDDFSKWNFYYSPQSSPDKKLTIFKTELRVRESDEETQIKVNWEEEAASGLLTSLKDNVPKATGVLYDYVNKYHWEHTGLTLREVSSKLRRNLQNNAEWVYQGAIRQIDDIDVRFQKAASGTTGTYQEWKDKAQNLYQELLTQEGQASFQGLKDNVFDGLVRVTQEFHMKVKHLIDSLIDFLNFPRFQFPGKPGIYTREELCTMFIREVGTVLSQVYSKVHNGSEILFSYFQDLVITLPFELRKHKLIDVISMYRELLKDLSKEAQEVFKAIQSLKTTEVLRNLQDLLQFIFQLIEDNIKQLKEMKFTYLINYIQDEINTIFSDYIPYVFKLLKENLCLNLHKFNEFIQNELQEASQELQQIHQYIMALREEYFDPSIVGWTVKYYELEEKIVSLIKNLLVALKDFHSEYIVSASNFTSQLSSQVEQFLHRNIQEYLSILTDPDGKGKEKIAELSATAQEIIKSQAIATKKIISDYHQQFRYKLQDFSDQLSDYYEKFIAESKRLIDLSIQNYHTFLIYITELLKKLQSTTVMNPYMKLAPGELTIIL
Enzyme Length 4563
Uniprot Accession Number P04114
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (2); Disulfide bond (8); Domain (1); Frameshift (1); Glycosylation (19); Lipidation (1); Modified residue (4); Mutagenesis (4); Natural variant (65); Region (9); Sequence conflict (53); Signal peptide (1)
Keywords Acetylation;Atherosclerosis;Cholesterol metabolism;Chylomicron;Cytoplasm;Direct protein sequencing;Disease variant;Disulfide bond;Glycoprotein;Heparin-binding;LDL;Lipid droplet;Lipid metabolism;Lipid transport;Lipoprotein;Palmitate;Phosphoprotein;RNA editing;Reference proteome;Secreted;Signal;Steroid metabolism;Sterol metabolism;Transport;VLDL
Interact With P01130; P55157; P29991
Induction INDUCTION: Up-regulated in response to enterovirus 71 (EV71) infection (at protein level). {ECO:0000269|PubMed:16548883}.
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22580899}. Secreted {ECO:0000269|PubMed:22580899, ECO:0000269|PubMed:26224785}. Lipid droplet {ECO:0000269|PubMed:28183703}.
Modified Residue MOD_RES 2004; /note="N6-acetyllysine"; /evidence="ECO:0007744|PubMed:19608861"; MOD_RES 3279; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:24275569"; MOD_RES 4048; /note="Phosphoserine; by FAM20C"; /evidence="ECO:0000269|PubMed:26091039, ECO:0007744|PubMed:24275569"; MOD_RES 4052; /note="Phosphothreonine"; /evidence="ECO:0007744|PubMed:24275569"
Post Translational Modification PTM: Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. {ECO:0000269|PubMed:10679026}.
Signal Peptide SIGNAL 1..27
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 10103054; 10191299; 10205247; 10224102; 10322811; 10339590; 10381560; 10535736; 10542231; 10559861; 10559924; 10625686; 10686426; 10722588; 10734051; 10766823; 10772654; 10852956; 10938017; 11060341; 11071065; 11074075; 11082044; 11170229; 11171287; 11181758; 11208426; 11231140; 11239472; 11247302; 11256994; 11341749; 11353333; 11359065; 11359462; 11369260; 11428473; 11459425; 11470803; 11471883; 11517213; 11532990; 11564734; 11568510; 11575217; 11577110; 11714857; 11715456; 11727199; 11737238; 11748351; 11781700; 11833852; 11840804; 11857554; 11861652; 11869298; 11882317; 11889126; 11898622; 11903341; 11940084; 11940087; 11947895; 11988484; 12006394; 12011770; 12031600; 12038793; 12044583; 12057195; 12070165; 12077138; 12105195; 12124991; 12130498; 12138091; 12150950; 12177061; 12206678; 12208478; 12221107; 12234931; 12235173; 12368141; 12373055; 12376530; 12397072; 12397634; 12417285; 12451172; 12464675; 12473645; 12488451; 12492446; 12505152; 12518019; 12529498; 12544508; 12547700; 12561593; 12576514; 12582154; 12626425; 12640502; 12651854; 12658354; 12670940; 12691171; 12692552; 12700342; 12700345; 12713147; 12736910; 12752570; 12760338; 12760590; 12761161; 12763022; 12774037; 12775720; 12778454; 1279089; 12818419; 12850958; 12872264; 12895336; 12901521; 12942366; 12944396; 12947091; 12952949; 12964943; 13130121; 1339697; 1346774; 14550622; 14567398; 14609958; 14618390; 14622578; 14622579; 14706682; 14726411; 14726519; 14732096; 14746139; 14763605; 14967814; 14967820; 15000751; 15028112; 15047470; 15076187; 15102883; 15111985; 15133863; 15135245; 15135251; 15135253; 15166224; 15186963; 15207528; 15208308; 15223114; 15252009; 15254032; 15256516; 15258202; 15260957; 15265030; 15318165; 15321837; 15321838; 15321839; 15345716; 15345724; 15355439; 15358600; 15371419; 15453913; 15459198; 15469418; 15477777; 15489541; 15502812; 15502813; 15504742; 15543563; 15561934; 15572036; 15576377; 15583000; 15585207; 15591219; 15591776; 15614026; 15616123; 15618547; 15630279; 15630635; 15637307; 15649145; 15653747; 15690042; 15699916; 15701167; 15703209; 15722561; 15728179; 15790761; 15795421; 15797858; 15823278; 15830122; 15856946; 15866482; 15867268; 15890894; 15895077; 15904989; 15918022; 16007035; 16015283; 16030169; 16103228; 16136540; 16152798; 16156669; 16159606; 16164023; 16179341; 16200213; 16201717; 16203724; 16215232; 16276419; 16316917; 16326391; 16368683; 16386541; 16389549; 16410077; 16412386; 16419488; 16455106; 16488891; 16516836; 16525840; 16544732; 16545386; 16596945; 16614317; 16620782; 16634094; 16670288; 16704977; 16758614; 16776623; 16792510; 16798879; 16801176; 1684378; 1685318; 16854991; 16895799; 16903783; 16920700; 16925469; 16926412; 16963757; 16978902; 16981218; 1698214; 16989840; 17018281; 17029200; 17032131; 17038630; 17043676; 17046772; 17071710; 17101731; 17109403; 17111197; 17129575; 17137217; 17142622; 17145755; 17158794; 17160438; 1718995; 17194460; 17214991; 17233885; 17237796; 17264219; 17277381; 17277465; 17288985; 17299378; 1732399; 17337758; 17339654; 17347910; 17349073; 17350490; 17367707; 17393087; 17393220; 17444660; 17457002; 17462654; 17463246; 17484618; 17502298; 17512409; 17530370; 17539906; 17540576; 17548929; 17566095; 17588943; 17595251; 17601988; 17609109; 17615582; 17633563; 17640344; 17644776; 17647275; 17659091; 17672218; 17681954; 17690102; 17696941; 17698027; 17698072; 17709607; 17710648; 1773531; 17765681; 17855807; 17876058; 17884995; 17890784; 1789809; 17962816; 17964958; 17978091; 18027103; 18028451; 18031309; 18067093; 18078691; 18078817; 18093523; 18160469; 18187430; 18193043; 18193044; 18193581; 18222178; 18254714; 18258526; 18262040; 18279815; 18296645; 18354102; 18387362; 18388068; 18388313; 18393233; 18434317; 18458151; 18473452; 1848237; 18492086; 18496130; 18499864; 18507396; 18512131; 18513389; 1852209; 18550891; 18558917; 1856611; 18566298; 18590467; 18597093; 18599996; 18632179; 18636124; 18644789; 18658047; 18660489; 18684539; 18696186; 18701171; 18710658; 18720163; 18725409; 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21502677; 21613550; 21640348; 21641598; 21722902; 21776394; 21779028; 21782039; 21801720; 21841790; 21868729; 21911577; 21927000; 21927001; 21946533; 21952877; 21958700; 21960651; 21962517; 21988832; 22028648; 22041135; 22074108; 22099461; 22105741; 22128163; 221835; 22211563; 22223728; 22233676; 22238364; 22247256; 22268108; 22301884; 22321874; 22341868; 22353362; 22363685; 22408029; 22437562; 22441101; 22443280; 22461740; 22471842; 22505844; 22507620; 2251254; 22517366; 22546827; 22570530; 22581286; 22607125; 22674204; 22717515; 22751103; 22760866; 22762727; 22786447; 22826357; 22827961; 22897442; 22905400; 22906437; 22913027; 22964994; 22985435; 23043354; 23053881; 23067240; 23121297; 23171143; 2318890; 23247145; 23297414; 23320904; 23359805; 23360385; 23391187; 23410546; 23444115; 23446247; 23457133; 23536704; 23588940; 23593297; 23631828; 23637837; 23669246; 23683938; 23685141; 23685223; 23697869; 23701995; 23723369; 23775634; 23823722; 23827315; 23861397; 23891661; 23986595; 24001780; 24069429; 24092915; 24100140; 24106285; 24136289; 24173238; 24186068; 24189400; 24234650; 24254975; 24265415; 24362231; 24362269; 24418085; 24447298; 24515109; 24581490; 24608795; 24680324; 24694356; 2472350; 24747110; 24789820; 24797048; 24816772; 24838241; 24842304; 24854276; 24859635; 24871656; 24882621; 24966051; 24972322; 25025299; 25070550; 25083581; 25107275; 25120028; 25159728; 25176030; 25201024; 25238218; 25248404; 25292352; 25341920; 25374385; 25391384; 25430706; 25432151; 25502789; 25539881; 25562991; 25575569; 25613532; 25614280; 25618028; 25699256; 25765953; 25768285; 25818853; 25837208; 25839937; 25861792; 25872266; 25875015; 25889118; 25911082; 25913481; 25953375; 25959453; 25961461; 26004908; 26025476; 26042225; 26071654; 26073401; 26118305; 26129832; 26188518; 26202245; 26323024; 26350813; 26363640; 26404905; 26446158; 26451733; 26496610; 26515419; 26537298; 26552755; 26561616; 26582246; 26612772; 26617857; 26637279; 26680362; 26748104; 26758372; 26802983; 26825690; 26842615; 26892134; 26916057; 26916732; 26945158; 26975530; 27055959; 27086565; 27112212; 27155339; 27172140; 27179706; 27210509; 2722848; 27236033; 27347637; 27468569; 27487947; 27637205; 27683205; 27751843; 27824107; 2793848; 28018102; 28106941; 28183448; 28235710; 28304324; 2833496; 28336556; 28370191; 28391908; 28428224; 28473443; 28482102; 28487135; 28502501; 28539357; 28540474; 28672200; 28677336; 28702990; 2872432; 28799493; 28826575; 28865324; 28902930; 28958330; 28988723; 28992985; 29032752; 29036232; 2904569; 2905601; 2906824; 29154726; 29244742; 29261184; 29353225; 29362515; 29374794; 29382324; 29440006; 29514644; 29540175; 29588316; 29776362; 29846435; 29883758; 29946054; 29956482; 30119683; 30191727; 3024002; 30242056; 30270084; 30400955; 30429453; 30488199; 30509867; 30649024; 30664017; 30681615; 30694837; 30755447; 30779793; 30782561; 30844855; 30939045; 30971288; 31135820; 31186542; 31409878; 31865929; 31932084; 31981042; 32044282; 32093271; 32130292; 32238407; 32243098; 32332433; 32407781; 32418956; 32493216; 32546165; 32591292; 32615719; 32629184; 32630105; 32654994; 32734598; 32770674; 32771037; 32776921; 32919080; 32950994; 33069457; 33418990; 33423974; 33604704; 33639994; 33794863; 33810957; 33810965; 33890362; 34125946; 34135129; 34202121; 34292395; 34419589; 3467373; 3473077; 34851955; 3942763; 4345202; 5057882; 6088540; 6146630; 6255257; 6619141; 7536797; 7607209; 7627691; 7647320; 7683668; 7685021; 7688729; 7803401; 7877694; 7908536; 8093429; 8112342; 8127896; 8146180; 8261646; 8275568; 8294473; 8300609; 8371062; 8429030; 8524399; 8546754; 8552632; 8626595; 8728311; 8751442; 8784195; 9052782; 9064379; 9092476; 9100026; 9148899; 9148942; 9211901; 9225214; 9254056; 9254062; 9395444; 9409200; 9427626; 9428629; 9465098; 9489234; 9511720; 9548586; 9548923; 9585673; 9603795; 9633939; 9635431; 9685400; 9693095; 9705237; 9706990; 9736607; 9763524; 9834113; 9867529; 9867530; 9880564; 9925661;
Motif
Gene Encoded By
Mass 515,605
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda