Detail Information for IndEnz0005000475
IED ID IndEnz0005000475
Enzyme Type ID lipase000475
Protein Name Alpha-L-fucosidase 3
EC 3.2.1.51
Alpha-1,2-fucosidase
AtFXG1
Alpha-L-fucoside fucohydrolase 3
Gene Name FXG1 At1g67830 F12A21.4
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MNPILSSLFALSLLSSLSPSTHAHQCHFPAIFNFGDSNSDTGGLSAAFGQAGPPHGSSFFGSPAGRYCDGRLVIDFIAESLGLPYLSAFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFSPFSLDVQFVQFYNFHNRSQTVRSRGGVYKTMLPESDSFSKALYTFDIGQNDLTAGYFANKTVEQVETEVPEIISQFMNAIKNIYGQGGRYFWIHNTGPIGCLAYVIERFPNKASDFDSHGCVSPLNHLAQQFNHALKQAVIELRSSLSEAAITYVDVYSLKHELFVHAQGHGFKGSLVSCCGHGGKYNYNKGIGCGMKKIVKGKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFDKIAPGLSKACKRQ
Enzyme Length 372
Uniprot Accession Number Q9FXE5
Absorption
Active Site ACT_SITE 37; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 345; /evidence=ECO:0000250; ACT_SITE 348; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=an alpha-L-fucoside + H2O = an alcohol + L-fucose; Xref=Rhea:RHEA:12288, ChEBI:CHEBI:2181, ChEBI:CHEBI:15377, ChEBI:CHEBI:28349, ChEBI:CHEBI:30879; EC=3.2.1.51; Evidence={ECO:0000269|PubMed:11788770};
DNA Binding
EC Number 3.2.1.51
Enzyme Function FUNCTION: Hydrolyzes alpha-1,2-linked fucose. Also active on fucosylated xyloglucan oligosaccharides. {ECO:0000269|PubMed:11788770}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Glycosylation (4); Sequence conflict (1); Signal peptide (1)
Keywords Apoplast;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast {ECO:0000269|PubMed:11788770, ECO:0000269|PubMed:16267099}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..23; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 17526915; 18650403; 22080600; 26344545; 28840447;
Motif
Gene Encoded By
Mass 40,462
Kinetics
Metal Binding
Rhea ID RHEA:12288
Cross Reference Brenda