Detail Information for IndEnz0005000478
IED ID IndEnz0005000478
Enzyme Type ID lipase000478
Protein Name Envelope phospholipase F13
EC 3.1.1.-
37 kDa protein
Envelope protein F13
Palmitoylated EV membrane protein
p37K
Gene Name F13L
Organism Vaccinia virus (strain Copenhagen) (VACV)
Taxonomic Lineage Viruses Varidnaviria Bamfordvirae Nucleocytoviricota Pokkesviricetes Chitovirales Poxviridae Chordopoxvirinae Orthopoxvirus Vaccinia virus Vaccinia virus (strain Copenhagen) (VACV)
Enzyme Sequence MWPFASVPAGAKCRLVETLPENMDFRSDHLTTFECFNEIITLAKKYIYIASFCCNPLSTTRGALIFDKLKEASEKGIKIIVLLDERGKRNLGELQSHCPDINFITVNIDKKNNVGLLLGCFWVSDDERCYVGNASFTGGSIHTIKTLGVYSDYPPLATDLRRRFDTFKAFNSAKNSWLNLCSAACCLPVSTAYHIKNPIGGVFFTDSPEHLLGYSRDLDTDVVIDKLRSAKTSIDIEHLAIVPTTRVDGNSYYWPDIYNSIIEAAINRGVKIRLLVGNWDKNDVYSMATARSLDALCVQNDLSVKVFTIQNNTKLLIVDDEYVHITSANFDGTHYQNHGFVSFNSIDKQLVSEAKKIFERDWVSSHSKSLKI
Enzyme Length 372
Uniprot Accession Number P20638
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.1.1.-
Enzyme Function FUNCTION: Major envelope protein that plays a role in the biogenesis of the viral double membrane and in egress of virus from the host cell. Produces the wrapped form of virus that is required for cell-to-cell spread. Acts as a lipase with broad specificity including phospholipase C, phospholipase A, and triacylglycerol lipase activities. {ECO:0000250|UniProtKB:P04021}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Domain (1); Motif (1)
Keywords Host Golgi apparatus;Host endoplasmic reticulum;Host membrane;Host-virus interaction;Hydrolase;Late protein;Lipoprotein;Membrane;Palmitate;Reference proteome;Viral budding;Viral budding via the host ESCRT complexes;Viral envelope protein;Viral release from host cell;Virion
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Virion membrane {ECO:0000250|UniProtKB:P04021}; Lipid-anchor {ECO:0000250|UniProtKB:P04021}. Host Golgi apparatus, host trans-Golgi network {ECO:0000250|UniProtKB:P04021}. Host endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P04021}; Lipid-anchor {ECO:0000250|UniProtKB:P04021}; Cytoplasmic side {ECO:0000250|UniProtKB:P04021}. Note=Component of the inner side of the enveloped virion (EV) membrane. F13 is associated post-translationally with membranes. {ECO:0000250|UniProtKB:P04021}.
Modified Residue
Post Translational Modification PTM: Palmitoylated. {ECO:0000250}.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif MOTIF 153..156; /note=YPPL
Gene Encoded By
Mass 41,824
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda