Detail Information for IndEnz0005000479
IED ID IndEnz0005000479
Enzyme Type ID lipase000479
Protein Name Envelope phospholipase F13
EC 3.1.1.-
37 kDa protein
Envelope protein F13
Palmitoylated EV membrane protein
p37K
Gene Name F5
Organism Vaccinia virus (strain L-IVP) (VACV)
Taxonomic Lineage Viruses Varidnaviria Bamfordvirae Nucleocytoviricota Pokkesviricetes Chitovirales Poxviridae Chordopoxvirinae Orthopoxvirus Vaccinia virus Vaccinia virus (strain L-IVP) (VACV)
Enzyme Sequence MWPFASVPAGAKCRLVETLPENMDFRSDHLTTFECFNEIITLAKKYIYIASFCCNPLSTTRGALIFDKLKEASEKGIKIIVLLDERGKRNLGELQSHCPDINFITVNIDKKNNVGLLLGCFWVSDDERCYVGNASFTGGSIHTIKTLGVYSDYPPLATDLRRRFDTFKAFDSAKNSWLNLCSAACCLPVSTAYHIKNPIGGVFFTDSPEHLLGYSRDLDTDVVIDKLKSAKTSIDIEHLAIVPTTRVDGNSYYWPDIYNSIIEAAINRGVKIRLLVGNWDKNDVYSMATARSLDALCVQNDLSVKVFTIQNNTKLLIVDDEYVHITSANFDGTHYQNHGFVSFNSIDKQLVSEAKKIFERDWVSSHSKSLKI
Enzyme Length 372
Uniprot Accession Number P29885
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.1.1.-
Enzyme Function FUNCTION: Major envelope protein that plays a role in the biogenesis of the viral double membrane and in egress of virus from the host cell. Produces the wrapped form of virus that is required for cell-to-cell spread. Acts as a lipase with broad specificity including phospholipase C, phospholipase A, and triacylglycerol lipase activities. {ECO:0000250|UniProtKB:P04021}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Domain (1); Motif (1)
Keywords Host Golgi apparatus;Host endoplasmic reticulum;Host membrane;Host-virus interaction;Hydrolase;Late protein;Lipoprotein;Membrane;Palmitate;Viral budding;Viral budding via the host ESCRT complexes;Viral envelope protein;Viral release from host cell;Virion
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Virion membrane {ECO:0000250|UniProtKB:P04021}; Lipid-anchor {ECO:0000250|UniProtKB:P04021}. Host Golgi apparatus, host trans-Golgi network {ECO:0000250|UniProtKB:P04021}. Host endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P04021}; Lipid-anchor {ECO:0000250|UniProtKB:P04021}; Cytoplasmic side {ECO:0000250|UniProtKB:P04021}. Note=Component of the inner side of the enveloped virion (EV) membrane. F13 is associated post-translationally with membranes. {ECO:0000250|UniProtKB:P04021}.
Modified Residue
Post Translational Modification PTM: Palmitoylated. {ECO:0000250}.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif MOTIF 153..156; /note=YPPL
Gene Encoded By
Mass 41,797
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda