| IED ID | IndEnz0005000482 |
| Enzyme Type ID | lipase000482 |
| Protein Name |
Carboxylesterase 3B EC 3.1.1.1 Liver carboxylesterase 31-like |
| Gene Name | Ces3b Gm4738 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MTNMRTMIPAGSSVLVWVTCLLLAFVTTVTGPKVIQPEVDTPLGRVRGRQVGVKDTDRMVNVFLGIPFAQAPVGPLRFSAPLPPQPWEGVRDASINPPMCLQDVEKMINSRFGLNEKIKIFPISEDCLTLNIYSPTEITAGDKRPVMVWIHGGSLLVGSSTSQDGSALAAYGDVVVVTVQYRLGIFGFLSTGDKHMPGNRGFLDVVAALRWVQGNIAPFGGDPNCVTIFGNSAGGMIVSSLFLSPISAGLFHRAISQSGIVTTIMMEDMKPWPEAQNFANSVACGSASPAELVQCLLQKEGKDLIKQKNVNISYIVNDSFFPQRPEKLLADQQFPTVPYLLGVTNHEFGWLLLKSLNILDKLEHLSREDLLEISRPFLAIMEVPPEIMPTVIDEYLDNGSDQSATRYAFQELLGDISFIIPTLNFSKYLRDAGCPVFLYEFQHTPSSFAKFKPAWVKADHASENSFVFGGPFLTDESSLLAFPEATEEEKQLSLTMMAQWSQFARTGNPNGKGLPPWPQLNQLEQYLEIGLESRTGVKLKKGRLQFWTETLPRKIQEWHREQRSRKVPEEL |
| Enzyme Length | 571 |
| Uniprot Accession Number | Q8VCU1 |
| Absorption | |
| Active Site | ACT_SITE 232; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 347; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 460; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10039}; |
| DNA Binding | |
| EC Number | 3.1.1.1 |
| Enzyme Function | FUNCTION: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (1); Chain (1); Disulfide bond (2); Erroneous initiation (2); Glycosylation (1); Motif (1); Signal peptide (1) |
| Keywords | Alternative splicing;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Hydrolase;Reference proteome;Serine esterase;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum lumen {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..31; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 20931200; 21677750; 7916639; |
| Motif | MOTIF 568..571; /note=Prevents secretion from ER; /evidence=ECO:0000255 |
| Gene Encoded By | |
| Mass | 63,353 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21164 |
| Cross Reference Brenda |