| IED ID | IndEnz0005000491 |
| Enzyme Type ID | lipase000491 |
| Protein Name |
3-O-acetylpapaveroxine carboxylesterase CXE2 EC 3.1.1.105 Carboxylesterase 2 |
| Gene Name | CXE2 |
| Organism | Papaver somniferum (Opium poppy) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Ranunculales Papaveraceae Papaveroideae Papaver Papaver somniferum (Opium poppy) |
| Enzyme Sequence | MADPYEFLMCIHDPEEDTLTRNFPIPATPLDQNTKDISLNLDRKTSLRIFRPPTEEFCVTTNKLLPIIIYFHGGGFVLFNADSTINHDFCQSIATHLPALVVSVDYRLAPENRLPAAYDDAVDALNWVKDQGLGKLNNSEVWLKEYGDFSKCFIMGCSSGGNIAYHASLRAIEMDLEPVIINGLILHSPFFGSLQRTESDLKVINDQDLPLAVRDVMWELALPLGSSRDHVYCNPNIANDGSSSGNMAGLIKRCLVIGFYGDPLIDRQIQLVKMLEEKGVKVETWIEQEGYHGVPCFDPKIRETLLGKIKYFI |
| Enzyme Length | 313 |
| Uniprot Accession Number | A0A0A1EQ07 |
| Absorption | |
| Active Site | ACT_SITE 158; /evidence=ECO:0000250|UniProtKB:Q5NUF3; ACT_SITE 262; /evidence=ECO:0000250|UniProtKB:Q5NUF3; ACT_SITE 292; /evidence=ECO:0000250|UniProtKB:Q5NUF3 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=3-O-acetylpapaveroxine + H2O = acetate + H(+) + narcotine hemiacetal; Xref=Rhea:RHEA:57400, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:141645, ChEBI:CHEBI:141667; EC=3.1.1.105; Evidence={ECO:0000269|PubMed:25485687};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57401; Evidence={ECO:0000305|PubMed:25485687}; |
| DNA Binding | |
| EC Number | 3.1.1.105 |
| Enzyme Function | FUNCTION: Carboxylesterase involved in the biosynthesis of the benzylisoquinoline alkaloid noscapine (PubMed:25485687). Converts 3-O-acetylpapaveroxine to narcotine hemiacetal (PubMed:25485687). {ECO:0000269|PubMed:25485687}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Alkaloid biosynthesis. {ECO:0000305}. |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Motif (1) |
| Keywords | Alkaloid metabolism;Hydrolase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 72..74; /note=Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole; /evidence=ECO:0000250|UniProtKB:Q5NUF3 |
| Gene Encoded By | |
| Mass | 35,154 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=25.2 uM for 3-O-acetylpapaveroxine {ECO:0000269|PubMed:25485687}; Vmax=1535 nmol/min/mg enzyme with 3-O-acetylpapaveroxine as substrate {ECO:0000269|PubMed:25485687}; |
| Metal Binding | |
| Rhea ID | RHEA:57400; RHEA:57401 |
| Cross Reference Brenda | 3.1.1.105; |