| IED ID | IndEnz0005000496 |
| Enzyme Type ID | lipase000496 |
| Protein Name |
Gut esterase 1 EC 3.1.1.1 Non-specific carboxylesterase |
| Gene Name | ges-1 R12A1.4 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MRIFLVSVILINACWAGPIVETNYGKVEGIEYEGAEVFLAIPFAKPPVDDLRFEKPVAPDPWEDVYPATQYRNDCTPHYRLVAQFSSYSGEDCLTLNIIKPKKAEKLPVLFWIHGGGYEIGSASQHGYEFFAKRYASQGVIVATVQYRLGFMGFFSEGTSDVQGNWGLFDQAAALEFVKSNIENFGGDPNQITIWGYSAGAASVSQLTMSPYTRDSYSKAIIMSASSFVGWATGPNVVETSKQLAEILGCPWPGAKECMKKKSLHEIFDAIEVQGWTTGTIDILRWSPVIDGDFMTKNPEELIKESPVKPTLIGMSNKEGSYFAALNMGRVIADFGLSPEDMPKVDEEFISEIIGRKLLYNNRYGENREKVWNDILDFYVKQGKPAEVKDLNGFYVDRYSELLSDITFNVPILREITSRVERKTPVWTYRMDHYDKNIWKKHIPEQARGSPHANEYHYLFDMPVMAKIDMKKEPDSWIQNDLIDMVISFAKTGVPQIEDVEWRPVSDPDDVNFLNIRSDGVSIEHGLFQEPLAFWNELRQREGFDLIDPTNSAMHSSNKDEL |
| Enzyme Length | 562 |
| Uniprot Accession Number | Q04457 |
| Absorption | |
| Active Site | ACT_SITE 198; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 319; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 452; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10039}; |
| DNA Binding | |
| EC Number | 3.1.1.1 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (2); Glycosylation (1); Motif (1); Signal peptide (1) |
| Keywords | Direct protein sequencing;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Hydrolase;Reference proteome;Serine esterase;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum lumen. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000269|PubMed:8445654 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11381264; 11784040; 12845331; 16777607; 18006689; 21177967; 21367940; 22286215; 22508763; 22560298; 2379823; 23800452; 25487147; 26009280; 29309033; 7649372; 8395047; |
| Motif | MOTIF 559..562; /note=Prevents secretion from ER |
| Gene Encoded By | |
| Mass | 63,722 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21164 |
| Cross Reference Brenda |