Detail Information for IndEnz0005000501
IED ID IndEnz0005000501
Enzyme Type ID lipase000501
Protein Name GDSL esterase/lipase EXL4
EC 3.1.1.-
Family II extracellular lipase 4
Family II lipase EXL4
Gene Name EXL4 At1g75910 T4O12.250
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MCSKITLVLTLFSSYFISTDAVNGSFPALLAFGDSILDTGNNNFLLTFMKGNIWPYGRSFSMRRATGRFGNGRVFSDIVAEGLGIKKILPAYRKLFNSPSDLRTGVCFASGGAGVDPVTSKLLRVLTPKDQVNDFKGYIRKLKATAGPSRASSIVSNAVILVSQGNNDIGISYFGTPTAAFRGLTPNRYTTKLAGWNKQFMKELYDQGARKFAVMGVIPLGCLPMTRIFLGGFVITCNFFANRVAEQYNGKLRSGTKSWGREAGFRGAKFVYVDMYNTLMDVIKNYRRYGFSNEKNGCCCMITAIIPCPNPDKYVFYDFVHPSEKAYRTISKKLVQDIKNGLA
Enzyme Length 343
Uniprot Accession Number Q0WUV7
Absorption
Active Site ACT_SITE 35; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 318; /evidence=ECO:0000250; ACT_SITE 321; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.1.1.-
Enzyme Function FUNCTION: Required for the formation of pollen coats and male fertility. {ECO:0000269|Ref.7}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Erroneous gene model prediction (1); Glycosylation (1); Sequence conflict (1); Signal peptide (1)
Keywords Direct protein sequencing;Extracellular matrix;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, pollen coat {ECO:0000269|PubMed:11431566, ECO:0000269|Ref.7}. Note=Localized in small granules in the tapetal cells.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..21; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 12777046; 15100403; 20033440; 28840447; 32374882; 7610479;
Motif
Gene Encoded By
Mass 37,911
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda