| IED ID | IndEnz0005000510 |
| Enzyme Type ID | lipase000510 |
| Protein Name |
Triacylglycerol lipase OBL1 EC 3.1.1.- Oil body lipase 1 AtOBL1 |
| Gene Name | OBL1 At3g14360 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MHKDNDSGSGSNPGQVSNYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDHRWVIVVSILVRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVIPERGSVTFVSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAYENAKVVENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFDADDWGTDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLLDMVERSAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFGQPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLFYDSFYNETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTHGPDYKEGWFRILFRLMGLVIPGLSDHCMTDYVNSVRLGPDNELQMSSL |
| Enzyme Length | 518 |
| Uniprot Accession Number | F4JFU8 |
| Absorption | |
| Active Site | ACT_SITE 339; /note=Nucleophile; /evidence=ECO:0000305|PubMed:29178188; ACT_SITE 403; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q948R1; ACT_SITE 496; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q948R1 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(9Z)-octadecenoate + 1,2-di-(9Z-octadecenoyl)-glycerol + H(+) = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O; Xref=Rhea:RHEA:38379, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:52323, ChEBI:CHEBI:53753; Evidence={ECO:0000269|PubMed:29178188};PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:38381; Evidence={ECO:0000269|PubMed:29178188}; CATALYTIC ACTIVITY: Reaction=1-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + glycerol + H(+); Xref=Rhea:RHEA:38487, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:30823, ChEBI:CHEBI:75342; Evidence={ECO:0000269|PubMed:29178188};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38488; Evidence={ECO:0000269|PubMed:29178188}; |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Acid lipase that can hydrolyze a range of triacylglycerols without a clear preference for acyl-chains (PubMed:29178188). Can also cleave 1,2-diacylglycerol, 1,3-diacylglycerol and 1-monoacylglycerol, but not phospatidylcholine, phosphatidylethanolamine, or sterol esters (PubMed:29178188). Required for pollen tube growth (PubMed:29178188). Triacylglycerol hydrolysis by OBL1 may provide acyl groups for the synthesis of membrane lipids in growing pollen tubes (Probable). {ECO:0000269|PubMed:29178188, ECO:0000305|PubMed:29178188}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.5. {ECO:0000269|PubMed:29178188}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Erroneous gene model prediction (1); Erroneous initiation (1); Motif (1); Mutagenesis (1); Sequence conflict (1); Transmembrane (1) |
| Keywords | Hydrolase;Lipid degradation;Lipid droplet;Lipid metabolism;Membrane;Reference proteome;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Lipid droplet {ECO:0000269|PubMed:29178188}. Membrane {ECO:0000255}; Single-pass membrane protein {ECO:0000255}. Note=Associates with the oil body membrane. {ECO:0000305|PubMed:29178188}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15937229; 17672910; 18775970; 23505340; |
| Motif | MOTIF 337..341; /note=GXSXG; /evidence=ECO:0000305|PubMed:29178188 |
| Gene Encoded By | |
| Mass | 59,607 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:38379; RHEA:38381; RHEA:38487; RHEA:38488 |
| Cross Reference Brenda |