Detail Information for IndEnz0005000522
IED ID IndEnz0005000522
Enzyme Type ID lipase000522
Protein Name Patatin-B2
EC 3.1.1.-
Gene Name PATB2
Organism Solanum tuberosum (Potato)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Solanoideae Solaneae Solanum Solanum tuberosum (Potato)
Enzyme Sequence MATTKSFLILFFMILATTSSTCAKLEEMVTVLSIDGGGIKGIIPAIILEFLEGQLQEVDNNKDARLADYFDVIGGTSTGGLLTAMITTPNENNRPFAAAKDIVPFYFEHGPHIFNYSGSILGPMYDGKYLLQVLQEKLGETRVHQALTEVAISSFDIKTNKPVIFTKSNLAKSPELDAKMYDICYSTAAAPIYFPPHHFVTHTSNGARYEFNLVDGAVATVGDPALLSLSVATRLAQEDPAFSSIKSLDYKQMLLLSLGTGTNSEFDKTYTAEEAAKWGPLRWMLAIQQMTNAASSYMTDYYISTVFQARHSQNNYLRVQENALNGTTTEMDDASEANMELLVQVGETLLKKPVSKDSPETYEEALKRFAKLLSDRKKLRANKASH
Enzyme Length 386
Uniprot Accession Number P15477
Absorption
Active Site ACT_SITE 77; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 215; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161
Activity Regulation ACTIVITY REGULATION: Inhibited by methyl-p-nitrophenyl-octylphosphonate.
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.1.1.-
Enzyme Function FUNCTION: Lipolytic acyl hydrolase (LAH), an activity which is thought to be involved in the response of tubers to pathogens. Can use p-nitrophenyl esters as substrates with highest activity on p-nitrophenyl caprate (C10). Also active on mono-acylglycolphosphocholines and diacylphospholipids, with highest specific activities observed were obtained with the synthetic phospholipids diC8PCho and diC9PCho. Mono-olein and myverol can also be hydrolyzed. {ECO:0000269|PubMed:11589694}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Domain (1); Glycosylation (2); Motif (3); Mutagenesis (6); Signal peptide (1)
Keywords Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Plant defense;Reference proteome;Signal;Storage protein;Vacuole
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Vacuole {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..23
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif MOTIF 36..41; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 75..79; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 215..217; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161
Gene Encoded By
Mass 42,613
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: Note=At pH 8.0, the highest specific activity is observed with phospholipids such as diC8PCho and diC9PCho and with p-nitrophenyl esters such as p-nitrophenyl caprate. {ECO:0000269|PubMed:11589694};
Metal Binding
Rhea ID
Cross Reference Brenda