| IED ID | IndEnz0005000552 |
| Enzyme Type ID | lipase000552 |
| Protein Name |
Lipid droplet-associated hydrolase EC 3.1.1.- Lipid droplet-associated serine hydrolase mLDAH |
| Gene Name | Ldah |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MASEVEEQIPVREEFFLCGGVETKIIKCGPWTNLFEKQDVSKPKQLIFIIPGNPGYSAFYVPFAKALYTLMKSRFPVWIISHAGFSVTPKDKKVLAAPQEESNAQKIEDVYGLNGQIEHKIAFLRAHVPKDVKLILIGHSVGTYMTLHVMKRVLELPVAHAFLLFPTIERMSESPNGKFATPFLCQFRYLLYATSYLLFKPCPEVIKSFIIQKLMGQMNIKLELPLTDILQPFCLANAAYLGSQEMVQIVKRDDDIIKEFLPKLKFYYGKTDGWCPVKYYEDMKKDFPEGNIYLCEKGIPHAFVLDFSQEMATIVAEWINNRPPRK |
| Enzyme Length | 326 |
| Uniprot Accession Number | Q8BVA5 |
| Absorption | |
| Active Site | ACT_SITE 140; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037; ACT_SITE 272; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q9H6V9; ACT_SITE 301; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q9H6V9 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Probable serine lipid hydrolase associated with lipid droplets (PubMed:24357060). Appears to lack cholesterol esterase activity (PubMed:24357060, PubMed:27836991). Appears to lack triglyceride lipase activity (PubMed:23525007, PubMed:24357060, PubMed:27836991). Highly expressed in macrophage-rich areas in atherosclerotic lesions, suggesting that it could promote cholesterol ester turnover in macrophages (PubMed:24357060). {ECO:0000269|PubMed:23525007, ECO:0000269|PubMed:24357060, ECO:0000269|PubMed:27836991}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (4); Chain (1); Mutagenesis (1); Sequence conflict (2) |
| Keywords | Alternative splicing;Endoplasmic reticulum;Hydrolase;Lipid droplet;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Lipid droplet {ECO:0000269|PubMed:23525007, ECO:0000269|PubMed:24357060}. Endoplasmic reticulum {ECO:0000269|PubMed:23525007}. Note=Localizes to the endoplasmic reticulum in absence of lipid droplets and translocates to lipid droplets upon lipid storage induction. {ECO:0000269|PubMed:23525007}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11217851; 12466851; 12520002; 14610273; 16919269; 17967808; 18799693; 21677750; 24194600; 27626380; 30169630; |
| Motif | |
| Gene Encoded By | |
| Mass | 37,374 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |