| IED ID | IndEnz0005000571 |
| Enzyme Type ID | lipase000571 |
| Protein Name |
Probable lipase MIL1 EC 3.1.1.- Medium adaptin-interacting ligand 1 |
| Gene Name | MIL1 YFL034W |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MSDSEEDLGVQLKGLKIARHLKESGEHTDEESNSSPEHDCGLSNQDDLTVMHTQAKEEVFKRREEDGTRTEDALHEGEAGKEGTGFPSSQSVCSPNEADSGIDRADKPILLDPFKSVHDTDPVPGTKSRSNSDSDSDSDDGGWQEMPAVSSFNIYNHRGELELTSKVRNSEQASETSPTVPPGKNCKSVNDSRFDYTKMAAEQQAQRSYRTNKKTDFLFDHKVLKKKINSSQTSVNLTSSPSTTSLNNEKNNDDDDDDSYDEYEDDVEPVNDLNRDSQLNITKNLLSDMEKFAYVGAINILANQMCTNLATLCLCIDIKSHKKLAHRLQFTQKDMAAWKTVVLSRLYDHLGISQEEIVMIEKLSLHKIQLEDLCKCLKTTQSIDNPWENDRDHEEDGIEETTERMSPNEQNGSVQASTPDPEQSATPETPKAKQSPLSSDVPGKVLDPENVKSQDKLNIDVAWTIICDLFLICLQSSTYDSRSRTLLINFAKVLNMTSLEICEFERRVTDSLDMEQSTEDQVWDEQDHMRNRRRSKRRKKMAYVALAMVGGSLVLGLSGGLLAPVIGGGIAAGLSTIGITGATSFLTGVGGTTVVAVSSTAIGANIGARGMSKRMGSVRTFEFRPLHNNRRVNLILTVSGWMVGNEDDVRLPFSTVDPVEGDLYSLYWEPEMLKSIGQTVSIVATEIFTTSLQQILGATVLTALISSIQWPMALSKLGYILDNPWNVSLDRAWSAGKILADTLIARNLGARPITLVGFSIGARVIFSCLIELCKKKALGLIENVYLFGTPAVMKKEQLVMARSVVSGRFVNGYSDKDWFLAYLFRAAAGGFSAVMGISTIENVEGIENINCTEFVDGHLNYRKSMPKLLKRIGIAVLSEEFVEIEEMMNPEEVKRKRKLINDVDAAQKKLSERKKHNSWVPKWLKPKKSKWKVMVEEAVEEGRDMQDLPENDVNNNENENPDEHEGIARQKRRDAALVDHGALMHELQLIKQAMHEDEIKNKACLPGEDKEVESSNDFLGESHYKPPSTPKINPPQSPNNFQLLSAGRTILPEDDDFDPRGKKKVEFSFPDDI |
| Enzyme Length | 1073 |
| Uniprot Accession Number | P43564 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Probable lipase that recruits the AP-1-related (AP-1R) complex to membranes via interaction with APM2 (PubMed:26658609). The AP-1R complex is an adapter protein complex that mediates of cargo protein SNC1 sorting in clathrin-coated vesicles (PubMed:26658609). {ECO:0000269|PubMed:26658609}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (9); Glycosylation (8); Modified residue (2); Motif (1); Mutagenesis (4); Region (6); Sequence conflict (1); Transmembrane (5) |
| Keywords | Cytoplasmic vesicle;Endosome;Glycoprotein;Golgi apparatus;Hydrolase;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000269|PubMed:26658609}; Multi-pass membrane protein {ECO:0000255}. Early endosome membrane {ECO:0000269|PubMed:26658609}; Multi-pass membrane protein {ECO:0000255}. Cytoplasmic vesicle, clathrin-coated vesicle membrane {ECO:0000269|PubMed:26658609}; Multi-pass membrane protein {ECO:0000255}. |
| Modified Residue | MOD_RES 435; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:19779198; MOD_RES 1037; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:18407956 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11805837; 15866509; 16429126; 16554755; 17646347; 19536198; 27462707; |
| Motif | MOTIF 143..147; /note=APM2-interacting WQEMP motif; /evidence=ECO:0000269|PubMed:26658609 |
| Gene Encoded By | |
| Mass | 119,510 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |