Detail Information for IndEnz0005000644
IED ID IndEnz0005000644
Enzyme Type ID lipase000644
Protein Name Putative acetyl-hydrolase LipR
EC 3.1.1.-
Gene Name lipR bah Rv3084
Organism Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycobacterium Mycobacterium tuberculosis complex Mycobacterium tuberculosis Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Enzyme Sequence MNLRKNVIRSVLRGARPLFASRRLGIAGRRVLLATLTAGARAPKGTRFQRVSIAGVPVQRVQPPHAATSGTLIYLHGGAYALGSARGYRGLAAQLAAAAGMTALVPDYTRAPHAHYPVALEEMAAVYTRLLDDGLDPKTTVIAGDSAGGGLTLALAMALRDRGIQAPAALGLICPWADLAVDIEATRPALRDPLILPSMCTEWAPRYVGSSDPRLPGISPVYGDMSGLPPIVMQTAGDDPICVDADKIETACAASKTSIEHRRFAGMWHDFHLQVSLLPEARDAIADLGARLRGHLHQSQGQPRGVVK
Enzyme Length 308
Uniprot Accession Number P9WK85
Absorption
Active Site ACT_SITE 146; /evidence=ECO:0000250|UniProtKB:O06350; ACT_SITE 239; /evidence=ECO:0000250|UniProtKB:O06350; ACT_SITE 269; /evidence=ECO:0000250|UniProtKB:O06350
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.1.1.-
Enzyme Function FUNCTION: Required for maintaining the appropriate mycolic acid composition and permeability of the envelope on its exposure to acidic pH. {ECO:0000269|PubMed:15937179}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Motif (1); Signal peptide (1)
Keywords Hydrolase;Reference proteome;Signal
Interact With
Induction INDUCTION: Expression is controlled by VirS. Induced at acidic pH and in macrophages. {ECO:0000269|PubMed:14568148}.
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..40; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif MOTIF 76..78; /note=Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole; /evidence=ECO:0000250|UniProtKB:Q5NUF3
Gene Encoded By
Mass 32,617
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda