Detail Information for IndEnz0005000666
IED ID IndEnz0005000666
Enzyme Type ID lipase000666
Protein Name Diacyltrehalose acyltransferase Chp2
EC 2.3.1.-
Gene Name chp2 Rv1184c RVBD_1184c LH57_06500 P425_01234
Organism Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycobacterium Mycobacterium tuberculosis complex Mycobacterium tuberculosis Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Enzyme Sequence MKRVIAGAFAVWLVGWAGGFGTAIAASEPAYPWAPGPPPSPSPVGDASTAKVVYALGGARMPGIPWYEYTNQAGSQYFPNAKHDLIDYPAGAAFSWWPTMLLPPGSHQDNMTVGVAVKDGTNSLDNAIHHGTDPAAAVGLSQGSLVLDQEQARLANDPTAPAPDKLQFTTFGDPTGRHAFGASFLARIFPPGSHIPIPFIEYTMPQQVDSQYDTNHVVTAYDGFSDFPDRPDNLLAVANAAIGAAIAHTPIGFTGPGDVPPQNIRTTVNSRGATTTTYLVPVNHLPLTLPLRYLGMSDAEVDQIDSVLQPQIDAAYARNDNWFTRPVSVDPVRGLDPLTAPGSIVEGARGLLGSPAFGG
Enzyme Length 359
Uniprot Accession Number O50440
Absorption
Active Site
Activity Regulation ACTIVITY REGULATION: Activity is probably potentiated by the DAT/PAT transporter MmpL10. Inhibited by the lipase inhibitor tetrahydrolipstatin (THL). {ECO:0000269|PubMed:25124040, ECO:0000269|PubMed:25331437}.
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 2.3.1.-
Enzyme Function FUNCTION: Involved in the final steps of polyacyltrehalose (PAT) biosynthesis. Catalyzes the transfer of three mycolipenoyl groups onto diacyltrehalose (DAT) to form PAT. {ECO:0000269|PubMed:25124040, ECO:0000269|PubMed:25331437}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Domain (1); Mutagenesis (1); Transmembrane (1)
Keywords Acyltransferase;Cell inner membrane;Cell membrane;Lipid biosynthesis;Lipid metabolism;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:25124040, ECO:0000269|PubMed:25331437}; Single-pass membrane protein {ECO:0000255}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 37,818
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda 2.3.1.284;