| IED ID | IndEnz0005000672 |
| Enzyme Type ID | lipase000672 |
| Protein Name |
Medium-chain fatty acid ethyl ester synthase/esterase 1 Alcohol O-acetyltransferase EC 2.3.1.84 EC 3.1.1.- Ethyl ester biosynthesis protein 1 |
| Gene Name | EEB1 YPL095C LPG13C |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MFRSGYYPTVTPSHWGYNGTVKHVLGEKGTKSLAFRDSKRQIPLHEFVTKHVPTLKDGANFRLNSLLFTGYLQTLYLSAGDFSKKFQVFYGREIIKFSDGGVCTADWVMPEWEQTYSLNAEKASFNEKQFSNDEKATHPKGWPRLHPRTRYLSSEELEKCHSKGYSYPLVVVLHGLAGGSHEPLIRALSEDLSKVGDGKFQVVVLNARGCSRSKVTTRRIFTALHTGDVREFLNHQKALFPQRKIYAVGTSFGAAMLTNYLGEEGDNCPLNAAVALSNPWDFVHTWDKLAHDWWSNHIFSRTLTQFLTRTVKVNMNELQVPENFEVSHKPTVEKPVFYTYTRENLEKAEKFTDILEFDNLFTAPSMGLPDGLTYYRKASSINRLPNIKIPTLIINATDDPVTGENVIPYKQARENPCVLLCETDLGGHLAYLDNESNSWLTKQAAEFLGSFDELVL |
| Enzyme Length | 456 |
| Uniprot Accession Number | Q02891 |
| Absorption | |
| Active Site | ACT_SITE 251; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 399; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 428; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=acetyl-CoA + an aliphatic alcohol = an acetyl ester + CoA; Xref=Rhea:RHEA:17229, ChEBI:CHEBI:2571, ChEBI:CHEBI:47622, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.84; |
| DNA Binding | |
| EC Number | 2.3.1.84; 3.1.1.- |
| Enzyme Function | FUNCTION: Displays enzymatic activity both for medium-chain fatty acid (MCFA) ethyl ester synthesis and hydrolysis (esterase activity). MCFA are toxic for yeast and this enzyme could thus be involved in their detoxification by esterification. {ECO:0000269|PubMed:16361250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (1) |
| Keywords | Acyltransferase;Hydrolase;Reference proteome;Serine esterase;Transferase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10688190; 11283351; 17993562; 18719252; 19536198; 19688096; 21255318; 22940803; 23464572; 24384752; |
| Motif | |
| Gene Encoded By | |
| Mass | 51,724 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:17229 |
| Cross Reference Brenda |