Detail Information for IndEnz0005000675
IED ID IndEnz0005000675
Enzyme Type ID lipase000675
Protein Name Triacylglycerol lipase OBL1
EC 3.1.1.-
Oil body lipase 1
NtOBL1
Gene Name OBL1 LOC107788962
Organism Nicotiana tabacum (Common tobacco)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Nicotianoideae Nicotianeae Nicotiana Nicotiana tabacum (Common tobacco)
Enzyme Sequence MASTKIDDKVSIPGPVTGTGNSRFLIVSHENGGIWDLVRFGVWGNKESGDKFLHYSAGGGLLEEHLVRSDDSGGGDDRGGEVPDHRWVIFVSIIVRKLIAIFGKPMEWTGYLVEFFLNLFSLNGNFLGLLYNILHGKVVMPHRGSETFISAIGHLDGRINLYKSETLTKEIGEPDFWQKIGIGHRDLMDLCMMASKLAYENEKVVRNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDANLILVSFRGTEPFDADDWITDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQLFVNNLKFANLENVHATIPPSESSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPERTAYYVVRSKLKRLLKEHKNAKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYGQPRVGNRQLGRFMEAHLEHPVPKYFRVVYCNDLVPRLPYDNKTFLFKHFGICQYYNSLYIEQNINEEPNPNYFGMRFLVPLYLNAGWELIRSFTMGYMYGSEYEECWESVMLRALGLFLPGISAHSPVDYVNSIRLGKARSTQMSSF
Enzyme Length 572
Uniprot Accession Number A0A1S3ZP85
Absorption
Active Site ACT_SITE 393; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:F4JFU8; ACT_SITE 457; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q948R1; ACT_SITE 550; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q948R1
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=(9Z)-octadecenoate + 1,2-di-(9Z-octadecenoyl)-glycerol + H(+) = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O; Xref=Rhea:RHEA:38379, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:52323, ChEBI:CHEBI:53753; Evidence={ECO:0000269|PubMed:29178188};PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:38381; Evidence={ECO:0000269|PubMed:29178188}; CATALYTIC ACTIVITY: Reaction=1-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + glycerol + H(+); Xref=Rhea:RHEA:38487, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:30823, ChEBI:CHEBI:75342; Evidence={ECO:0000269|PubMed:29178188};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38488; Evidence={ECO:0000269|PubMed:29178188};
DNA Binding
EC Number 3.1.1.-
Enzyme Function FUNCTION: Acid lipase that can hydrolyze a range of triacylglycerols without a clear preference for acyl-chains (PubMed:29178188). Can also cleave 1,2-diacylglycerol, 1,3-diacylglycerol and 1-monoacylglycerol, but not phospatidylcholine, phosphatidylethanolamine, or sterol esters (PubMed:29178188). Required for pollen tube growth (PubMed:29178188). Triacylglycerol hydrolysis by OBL1 may provide acyl groups for the synthesis of membrane lipids in growing pollen tubes (Probable). {ECO:0000269|PubMed:29178188, ECO:0000305|PubMed:29178188}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Compositional bias (2); Motif (1); Mutagenesis (1); Region (1); Transmembrane (1)
Keywords Hydrolase;Lipid degradation;Lipid droplet;Lipid metabolism;Membrane;Reference proteome;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Lipid droplet {ECO:0000269|PubMed:29178188}. Membrane {ECO:0000255}; Single-pass membrane protein {ECO:0000255}. Note=Associates with the oil body membrane. {ECO:0000305|PubMed:29178188}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif MOTIF 391..395; /note=GXSXG; /evidence=ECO:0000250|UniProtKB:F4JFU8
Gene Encoded By
Mass 65,270
Kinetics
Metal Binding
Rhea ID RHEA:38379; RHEA:38381; RHEA:38487; RHEA:38488
Cross Reference Brenda