| IED ID | IndEnz0005000696 |
| Enzyme Type ID | lipase000696 |
| Protein Name |
Phospholipase A1-Ibeta2, chloroplastic EC 3.1.1.- |
| Gene Name | At4g16820 dl4435w FCAALL.42 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MQTLTPNADIFHAKRRRFTCNTHTSTLIPTKPLSVSPARKTNKEHLRNLENVLRTSSNSIDHIENVTSRQEKTTKNTSTSSLLGGLNLARIWPQMKAAVDEMSPKNLKRLQRLLSKSSEERSPKSKLGSKWRELHGLNNWAGLLDPLDENLRRELVRYGEFVQAAYHAFHSDPEGSPRHVALPDGSFKVTKSLYATSSVRLPKWIDDVAPDLRWMTKQTSWVGYVAVCDDPREIRRMGRREIVIALRGTATLLEWSENFRPNLVSMPEPKPDQSDPTRPKVECGFNSLYTTGDQHAPSLAESLVGEISRLVELYAGEELSISVTGHSLGAAIALLAADDIAERVPHAPPVAVFSFGGPRVGNREFADRLDSKGVKVLRVVNSQDVVTKVPGIFADNDKQGQSRNNGRSPGGIMEMVERNNPWAYSHVGAELRVDMKMSPYLKPNADVACCHDLEAYLHLVDGFLASNCPFRANAKRSLRKLLDEQRSNVKVLYTGKSLRLNRTFHDNGDVLPSPSSS |
| Enzyme Length | 517 |
| Uniprot Accession Number | O23522 |
| Absorption | |
| Active Site | ACT_SITE 327; /note=Acyl-ester intermediate; /evidence=ECO:0000255; ACT_SITE 384; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037; ACT_SITE 451; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Acylhydrolase that catalyzes the hydrolysis of phosphatidylcholine at the sn-1 position. Has a strong galactolipase activity toward digalactosyldiacylglycerol (DGDG). Hydrolyzes triacylglycerol (TAG), but has a low activity toward phosphatidylcholine (PC) and monogalactosyldiacylglycerol (MGDG). |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Cross-link (1); Erroneous gene model prediction (2); Transit peptide (1) |
| Keywords | Chloroplast;Hydrolase;Isopeptide bond;Lipid degradation;Lipid metabolism;Plastid;Reference proteome;Transit peptide;Ubl conjugation |
| Interact With | |
| Induction | INDUCTION: Not induced by wounding. {ECO:0000269|PubMed:18267087}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12805597; 14576160; 15235117; 15923325; 16510518; 18667720; 20855949; 22442409; 23505340; 24430866; |
| Motif | |
| Gene Encoded By | |
| Mass | 57,640 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |