Detail Information for IndEnz0005000711
IED ID IndEnz0005000711
Enzyme Type ID lipase000711
Protein Name 3-
3-hydroxydecanoyloxy
decanoate synthase
EC 2.3.1.-
Gene Name rhlA PA3479
Organism Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa group Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Enzyme Sequence MRRESLLVSVCKGLRVHVERVGQDPGRSTVMLVNGAMATTASFARTCKCLAEHFNVVLFDLPFAGQSRQHNPQRGLITKDDEVEILLALIERFEVNHLVSASWGGISTLLALSRNPRGIRSSVVMAFAPGLNQAMLDYVGRAQALIELDDKSAIGHLLNETVGKYLPQRLKASNHQHMASLATGEYEQARFHIDQVLALNDRGYLACLERIQSHVHFINGSWDEYTTAEDARQFRDYLPHCSFSRVEGTGHFLDLESKLAAVRVHRALLEHLLKQPEPQRAERAAGFHEMAIGYA
Enzyme Length 295
Uniprot Accession Number Q51559
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=2 (3R)-hydroxydecanoyl-[ACP] + H2O = (3R)-3-[(3R)-3-hydroxydecanoyloxy]decanoate + H(+) + 2 holo-[ACP]; Xref=Rhea:RHEA:55592, Rhea:RHEA-COMP:9638, Rhea:RHEA-COMP:9685, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:64479, ChEBI:CHEBI:78466, ChEBI:CHEBI:139104; Evidence={ECO:0000269|PubMed:18326581};
DNA Binding
EC Number 2.3.1.-
Enzyme Function FUNCTION: Required for rhamnolipid surfactant production (PubMed:15126453). Supplies the acyl moieties for rhamnolipid biosynthesis by competing with the enzymes of the type II fatty acid synthase (FASII) cycle for the beta-hydroxyacyl-acyl carrier protein (ACP) pathway intermediates. Catalyzes the formation of one molecule of beta-hydroxydecanoyl-beta-hydroxydecanoate from two molecules of beta-hydroxydecanoyl-ACP. Is the only enzyme required to generate the lipid component of rhamnolipid. In vitro results establish that RhlA is highly selective for 10-carbon acyl-ACP intermediates and thus functions as the molecular ruler that controls the acyl chain composition of rhamnolipids. Cannot use beta-hydroxydecanoyl-CoA as substrate (PubMed:18326581). Rhamnolipid production plays an important role in swarming motility (PubMed:15126453). {ECO:0000269|PubMed:15126453, ECO:0000269|PubMed:18326581}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Lipid metabolism; rhamnolipid biosynthesis. {ECO:0000269|PubMed:18326581}.
nucleotide Binding
Features Chain (1); Domain (1); Sequence conflict (1)
Keywords Acyltransferase;Lipid biosynthesis;Lipid metabolism;Reference proteome;Stress response;Transferase
Interact With
Induction INDUCTION: By nitrogen starvation (PubMed:8051059). Expression is about 20-fold higher in stationary phase (PubMed:8051059). Positively regulated by the translational regulator CsrA (RsmA) (PubMed:15126453). {ECO:0000269|PubMed:15126453, ECO:0000269|PubMed:8051059}.
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 32,963
Kinetics
Metal Binding
Rhea ID RHEA:55592
Cross Reference Brenda