| IED ID | IndEnz0005000719 |
| Enzyme Type ID | lipase000719 |
| Protein Name |
Phospholipase A2 group XV 1-O-acylceramide synthase ACS LCAT-like lysophospholipase LLPL EC 3.1.1.5 Lysophospholipase 3 Lysosomal phospholipase A and acyltransferase EC 2.3.1.- EC 3.1.1.32 EC 3.1.1.4 Lysosomal phospholipase A2 LPLA2 |
| Gene Name | PLA2G15 LYPLA3 |
| Organism | Bos taurus (Bovine) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Ruminantia Pecora Bovidae Bovinae Bos (oxen cattle) Bos taurus (Bovine) |
| Enzyme Sequence | MGCLCLYRSTLLTGGLLFLLMLADPAFPAGSRPPVVLVPGDMGNQLEAKLDKPSVVHYVCSKRTDHYFTLWLNLELLLPVIIDCWIDNVRLIYNQTSHTTQFPEGVDVRVPGFGDTFSMEFLDPSKSSVGSYLHTMVESLVSWGYERGKDVRGAPYDWRRAPNENGPYFLALRKMIEEMYQLYGGPVVLVAHSMGNMYMLYFLQHQPQDWKDKYIRAFVALGPPWGGVPKTLRVLASGDNNRIPVIRSLKIRAQQRSAVSTTWLLPYSYTWSPQKVFVRTPKANYTLQDYRQFFQDIGFKDGWSMRQDTEGLVEATVPPGVRLHCLYGTGVPTPESFDYESFPDRDPKIHYGTGDGTVNLQSALHCHTWRGLQKQEVSLQALPGNEHIAMLANTTTLAYLKRVLLGP |
| Enzyme Length | 407 |
| Uniprot Accession Number | Q8WMP9 |
| Absorption | |
| Active Site | ACT_SITE 193; /note="Acyl-ester intermediate"; /evidence="ECO:0000250|UniProtKB:Q8NCC3, ECO:0000255"; ACT_SITE 355; /note="Charge relay system"; /evidence="ECO:0000250|UniProtKB:Q8NCC3"; ACT_SITE 387; /note="Charge relay system"; /evidence="ECO:0000250|UniProtKB:Q8NCC3" |
| Activity Regulation | ACTIVITY REGULATION: Transacylase activity is completely inhibited by Triton X-100 and partially inhibited by heparin. Moderately activated by Mg(2+) and Ca(2+). {ECO:0000269|PubMed:9525960}. |
| Binding Site | BINDING 41; /note=Substrate; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:Q8NCC3; BINDING 194; /note=Substrate; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:Q8NCC3 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:18689, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:57643, ChEBI:CHEBI:57875; EC=3.1.1.32; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:18690; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H(+) + hexadecanoate; Xref=Rhea:RHEA:38783, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:73001, ChEBI:CHEBI:76071; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38784; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-glutaroyl-sn-glycero-3-phosphocholine + H2O = 2-glutaroyl-sn-glycero-3-phosphocholine + H(+) + hexadecanoate; Xref=Rhea:RHEA:62480, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:77756, ChEBI:CHEBI:145781; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62481; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-nonadioyl-sn-glycero-3-phosphocholine + H2O = 2-nonadioyl-sn-glycero-3-phosphocholine + H(+) + hexadecanoate; Xref=Rhea:RHEA:62464, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:78207, ChEBI:CHEBI:145780; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62465; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(5-oxopentanoyl)-sn-glycero-3-phosphocholine + H2O = 2-(5-oxopentanoyl)-sn-glycero-3-phosphocholine + H(+) + hexadecanoate; Xref=Rhea:RHEA:62484, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:77890, ChEBI:CHEBI:145782; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62485; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9-oxononanoyl)-sn-glycero-3-phosphocholine + H2O = 2-(9-oxononanoyl)-sn-glycero-3-phosphocholine + H(+) + hexadecanoate; Xref=Rhea:RHEA:62488, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:61042, ChEBI:CHEBI:145783; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62489; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:15801, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:57643, ChEBI:CHEBI:58168; EC=3.1.1.4; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15802; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = (9Z)-octadecenoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+); Xref=Rhea:RHEA:38779, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:72998, ChEBI:CHEBI:73001; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38780; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15178; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = H(+) + hexadecanoate + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:40435, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:72998; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40436; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:38827, ChEBI:CHEBI:46979, ChEBI:CHEBI:73007, ChEBI:CHEBI:76077, ChEBI:CHEBI:76088; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38828; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:38823, ChEBI:CHEBI:46979, ChEBI:CHEBI:73004, ChEBI:CHEBI:73007, ChEBI:CHEBI:76054; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38824; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z,12Z)-octadecadienoyl-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:38831, ChEBI:CHEBI:46979, ChEBI:CHEBI:73008, ChEBI:CHEBI:76077, ChEBI:CHEBI:76090; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38832; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine + N-(acetyl)-sphing-4-enine = 1-(9Z,12Z-octadecadienoyl)-N-acetylsphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:38835, ChEBI:CHEBI:46979, ChEBI:CHEBI:73004, ChEBI:CHEBI:73008, ChEBI:CHEBI:76086; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38836; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:38843, ChEBI:CHEBI:46979, ChEBI:CHEBI:73009, ChEBI:CHEBI:76077, ChEBI:CHEBI:76091; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38844; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + N-(acetyl)-sphing-4-enine = 1-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-N-(acetyl)-sphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:38839, ChEBI:CHEBI:46979, ChEBI:CHEBI:73004, ChEBI:CHEBI:73009, ChEBI:CHEBI:76080; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38840; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38759, ChEBI:CHEBI:46979, ChEBI:CHEBI:73001, ChEBI:CHEBI:76071, ChEBI:CHEBI:76077; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38760; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38755, ChEBI:CHEBI:46979, ChEBI:CHEBI:72998, ChEBI:CHEBI:73001, ChEBI:CHEBI:76054; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38756; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38811, ChEBI:CHEBI:46979, ChEBI:CHEBI:73002, ChEBI:CHEBI:76077, ChEBI:CHEBI:76084; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38812; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z,12Z-octadecadienoyl)-N-acetylsphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38807, ChEBI:CHEBI:46979, ChEBI:CHEBI:72998, ChEBI:CHEBI:73002, ChEBI:CHEBI:76086; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38808; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38775, ChEBI:CHEBI:46979, ChEBI:CHEBI:73003, ChEBI:CHEBI:76077, ChEBI:CHEBI:76079; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38776; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-N-(acetyl)-sphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38771, ChEBI:CHEBI:46979, ChEBI:CHEBI:72998, ChEBI:CHEBI:73003, ChEBI:CHEBI:76080; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38772; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38815, ChEBI:CHEBI:46979, ChEBI:CHEBI:74963, ChEBI:CHEBI:76077, ChEBI:CHEBI:76085; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38816; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-N-(acetyl)-sphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38819, ChEBI:CHEBI:46979, ChEBI:CHEBI:72998, ChEBI:CHEBI:74963, ChEBI:CHEBI:76087; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38820; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-nonadioyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-nonadioyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:62472, ChEBI:CHEBI:46979, ChEBI:CHEBI:76077, ChEBI:CHEBI:78207, ChEBI:CHEBI:145780; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62473; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-octadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38799, ChEBI:CHEBI:46979, ChEBI:CHEBI:75034, ChEBI:CHEBI:76071, ChEBI:CHEBI:76074; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38800; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 1-octadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38795, ChEBI:CHEBI:46979, ChEBI:CHEBI:73858, ChEBI:CHEBI:75034, ChEBI:CHEBI:76054; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38796; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z,12Z)-octadecadienoyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z,12Z-octadecadienoyl)-N-acetylsphing-4-enine + 1-octadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:57108, ChEBI:CHEBI:46979, ChEBI:CHEBI:73858, ChEBI:CHEBI:76086, ChEBI:CHEBI:84822; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57109; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-octadecanoyl-N-(acetyl)-sphing-4-enine + 2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:57120, ChEBI:CHEBI:46979, ChEBI:CHEBI:74965, ChEBI:CHEBI:76074, ChEBI:CHEBI:76079; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57121; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-N-(acetyl)-sphing-4-enine + 1-octadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:57116, ChEBI:CHEBI:46979, ChEBI:CHEBI:73858, ChEBI:CHEBI:74965, ChEBI:CHEBI:76080; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57117; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-(9Z-octadecenoyl)-2-hexadecanoyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 2-hexadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38767, ChEBI:CHEBI:46979, ChEBI:CHEBI:74667, ChEBI:CHEBI:76054, ChEBI:CHEBI:76078; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38768; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-(9Z-octadecenoyl)-2-hexadecanoyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-hexadecanoyl-N-(acetyl)-sphing-4-enine; Xref=Rhea:RHEA:38763, ChEBI:CHEBI:28610, ChEBI:CHEBI:46979, ChEBI:CHEBI:74667, ChEBI:CHEBI:76077; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38764; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-(9Z)-octadecenoyl-2-octadecanoyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 2-octadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38791, ChEBI:CHEBI:46979, ChEBI:CHEBI:76054, ChEBI:CHEBI:76073, ChEBI:CHEBI:76076; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38792; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-(9Z)-octadecenoyl-2-octadecanoyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-octadecanoyl-N-(acetyl)-sphing-4-enine; Xref=Rhea:RHEA:38803, ChEBI:CHEBI:28610, ChEBI:CHEBI:46979, ChEBI:CHEBI:76073, ChEBI:CHEBI:76074; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38804; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38703, ChEBI:CHEBI:28610, ChEBI:CHEBI:46979, ChEBI:CHEBI:74669, ChEBI:CHEBI:76054; Evidence={ECO:0000269|PubMed:11790796, ECO:0000269|PubMed:9525960};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38704; Evidence={ECO:0000305|PubMed:11790796, ECO:0000305|PubMed:9525960}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + N-(acetyl)-sphing-4-enine = 1-octadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine; Xref=Rhea:RHEA:57140, ChEBI:CHEBI:46979, ChEBI:CHEBI:76074, ChEBI:CHEBI:77342, ChEBI:CHEBI:78260; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57141; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 1-octadecanoyl-sn-glycero-3-phosphoserine; Xref=Rhea:RHEA:57136, ChEBI:CHEBI:46979, ChEBI:CHEBI:76054, ChEBI:CHEBI:78260, ChEBI:CHEBI:84467; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57137; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + N-(acetyl)-sphing-4-enine = 1-octadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol); Xref=Rhea:RHEA:57144, ChEBI:CHEBI:46979, ChEBI:CHEBI:72845, ChEBI:CHEBI:76074, ChEBI:CHEBI:141490; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57145; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 1-octadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol); Xref=Rhea:RHEA:57148, ChEBI:CHEBI:46979, ChEBI:CHEBI:72827, ChEBI:CHEBI:72845, ChEBI:CHEBI:76054; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57149; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 2-[(5Z,8Z,11Z,14Z)-eicosatetraenoylamino]ethyl (9Z)-octadecenoate; Xref=Rhea:RHEA:38751, ChEBI:CHEBI:2700, ChEBI:CHEBI:74669, ChEBI:CHEBI:76070, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38752; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(9Z-octadecenoyl) ethanolamine = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 2-[(9Z)-octadecenoylamino]ethyl (9Z)-octadecenoate; Xref=Rhea:RHEA:38747, ChEBI:CHEBI:71466, ChEBI:CHEBI:74669, ChEBI:CHEBI:76068, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38748; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 3-(9Z-octadecenoyl)-sn-glycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1,3-di-(9Z-octadecenoyl)-glycerol; Xref=Rhea:RHEA:38743, ChEBI:CHEBI:74669, ChEBI:CHEBI:75735, ChEBI:CHEBI:75938, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38744; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-(9Z-octadecenoyl)-sn-glycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1,3-di-(9Z-octadecenoyl)-glycerol; Xref=Rhea:RHEA:38739, ChEBI:CHEBI:74669, ChEBI:CHEBI:75735, ChEBI:CHEBI:75757, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38740; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 2-hexadecanoylglycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-(9Z)-octadecenoyl-2-hexadecanoylglycerol; Xref=Rhea:RHEA:38735, ChEBI:CHEBI:74669, ChEBI:CHEBI:75455, ChEBI:CHEBI:76065, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38736; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 3-hexadecanoyl-sn-glycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol; Xref=Rhea:RHEA:38731, ChEBI:CHEBI:64757, ChEBI:CHEBI:74669, ChEBI:CHEBI:75867, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38732; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = (9Z)-octadecenoate + (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H(+); Xref=Rhea:RHEA:38699, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:74669, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38700; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-O-hexadecyl-2-acetyl-sn-glycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-O-hexadecyl-2-acetyl-3-(9Z)-octadecenoyl-sn-glycerol; Xref=Rhea:RHEA:38707, ChEBI:CHEBI:74669, ChEBI:CHEBI:75936, ChEBI:CHEBI:76055, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38708; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-O-hexadecylglycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-O-hexadecyl-3-(9Z)-octadecenoylglycerol; Xref=Rhea:RHEA:38711, ChEBI:CHEBI:74669, ChEBI:CHEBI:76061, ChEBI:CHEBI:76062, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38712; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-O-hexadecyl-2-O-methyl-sn-glycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-O-hexadecyl-2-O-methyl-3-(9Z)-octadecenoyl-sn-glycerol; Xref=Rhea:RHEA:38723, ChEBI:CHEBI:74669, ChEBI:CHEBI:76063, ChEBI:CHEBI:76064, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38724; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-hexadecanoyl-sn-glycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-hexadecanoyl-3-(9Z)-octadecenoyl-sn-glycerol; Xref=Rhea:RHEA:38727, ChEBI:CHEBI:74669, ChEBI:CHEBI:75542, ChEBI:CHEBI:75868, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38728; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; |
| DNA Binding | |
| EC Number | 3.1.1.5; 2.3.1.-; 3.1.1.32; 3.1.1.4 |
| Enzyme Function | FUNCTION: Has dual calcium-independent phospholipase and O-acyltransferase activities with a potential role in glycerophospholipid homeostasis and remodeling of acyl groups of lipophilic alcohols present in acidic cellular compartments (PubMed:11790796, PubMed:9525960). Catalyzes hydrolysis of the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 or A2 activity) and transfer it to the hydroxyl group at the first carbon of lipophilic alcohols (O-acyltransferase activity) (PubMed:11790796, PubMed:9525960). Among preferred fatty acyl donors are phosphatidylcholines, phosphatidylethanolamines, phosphatidylglycerols and phosphatidylserines (By similarity). Favors sn-2 over sn-1 deacylation of unsaturated fatty acyl groups of phosphatidylcholines and phosphatidylethanolamines (By similarity). Among preferred fatty acyl acceptors are natural lipophilic alcohols including short-chain ceramide N-acetyl-sphingosine (C2 ceramide), alkylacylglycerols, monoacylglycerols, and acylethanolamides such as anandamide and oleoylethanolamide (By similarity). Selectively hydrolyzes the sn-1 fatty acyl group of truncated oxidized phospholipids and may play a role in detoxification of reactive oxidized phospholipids during oxidative stress. Required for normal phospholipid degradation in alveolar macrophages with potential implications in pulmonary surfactant clearance (By similarity). At neutral pH, hydrolyzes the sn-1 fatty acyl group of the lysophosphatidylcholines (By similarity). {ECO:0000250|UniProtKB:Q8NCC3, ECO:0000250|UniProtKB:Q8VEB4, ECO:0000269|PubMed:11790796, ECO:0000269|PubMed:9525960}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4.5. {ECO:0000269|PubMed:9525960}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Binding site (2); Chain (1); Disulfide bond (1); Glycosylation (3); Metal binding (2); Sequence conflict (1); Signal peptide (1) |
| Keywords | Acyltransferase;Direct protein sequencing;Disulfide bond;Fatty acid metabolism;Glycoprotein;Hydrolase;Lipid metabolism;Lysosome;Membrane;Metal-binding;Reference proteome;Secreted;Signal;Transferase;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Lysosome {ECO:0000250|UniProtKB:Q6XPZ3}. Secreted {ECO:0000250|UniProtKB:Q8NCC3}. Membrane {ECO:0000250|UniProtKB:Q8NCC3}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q8NCC3}. |
| Modified Residue | |
| Post Translational Modification | PTM: N-glycosylated. {ECO:0000269|PubMed:11790796, ECO:0000269|PubMed:9525960}.; PTM: N-glycosylated. N-glycosylation is important for maturation of the enzyme and normal subcellular location. {ECO:0000250|UniProtKB:Q8NCC3}. |
| Signal Peptide | SIGNAL 1..29; /evidence=ECO:0000269|PubMed:11790796 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 46,062 |
| Kinetics | |
| Metal Binding | METAL 193; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q8NCC3; METAL 387; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q8NCC3 |
| Rhea ID | RHEA:18689; RHEA:18690; RHEA:38783; RHEA:38784; RHEA:62480; RHEA:62481; RHEA:62464; RHEA:62465; RHEA:62484; RHEA:62485; RHEA:62488; RHEA:62489; RHEA:15801; RHEA:15802; RHEA:38779; RHEA:38780; RHEA:15177; RHEA:15178; RHEA:40435; RHEA:40436; RHEA:38827; RHEA:38828; RHEA:38823; RHEA:38824; RHEA:38831; RHEA:38832; RHEA:38835; RHEA:38836; RHEA:38843; RHEA:38844; RHEA:38839; RHEA:38840; RHEA:38759; RHEA:38760; RHEA:38755; RHEA:38756; RHEA:38811; RHEA:38812; RHEA:38807; RHEA:38808; RHEA:38775; RHEA:38776; RHEA:38771; RHEA:38772; RHEA:38815; RHEA:38816; RHEA:38819; RHEA:38820; RHEA:62472; RHEA:62473; RHEA:38799; RHEA:38800; RHEA:38795; RHEA:38796; RHEA:57108; RHEA:57109; RHEA:57120; RHEA:57121; RHEA:57116; RHEA:57117; RHEA:38767; RHEA:38768; RHEA:38763; RHEA:38764; RHEA:38791; RHEA:38792; RHEA:38803; RHEA:38804; RHEA:38703; RHEA:38704; RHEA:57140; RHEA:57141; RHEA:57136; RHEA:57137; RHEA:57144; RHEA:57145; RHEA:57148; RHEA:57149; RHEA:38751; RHEA:38752; RHEA:38747; RHEA:38748; RHEA:38743; RHEA:38744; RHEA:38739; RHEA:38740; RHEA:38735; RHEA:38736; RHEA:38731; RHEA:38732; RHEA:38699; RHEA:38700; RHEA:38707; RHEA:38708; RHEA:38711; RHEA:38712; RHEA:38723; RHEA:38724; RHEA:38727; RHEA:38728 |
| Cross Reference Brenda |