| IED ID | IndEnz0005000724 |
| Enzyme Type ID | lipase000724 |
| Protein Name |
Variant-surface-glycoprotein phospholipase C VSG lipase EC 4.6.1.14 Glycosylphosphatidylinositol-specific phospholipase C GPI-PLC |
| Gene Name | |
| Organism | Trypanosoma brucei brucei |
| Taxonomic Lineage | cellular organisms Eukaryota Discoba Euglenozoa Kinetoplastea (kinetoplasts) Metakinetoplastina Trypanosomatida Trypanosomatidae Trypanosoma Trypanozoon Trypanosoma brucei Trypanosoma brucei brucei |
| Enzyme Sequence | MFGGVKWSPQSWMSDTRSSIEKKCIGQVYMVGAHNAGTHGIQMFSPFGLDAPEKLRSLPPYVTFLLRFLTVGVSSRWGRCQNLSIRQLLDHGVRYLDLRMNISPDQENKIYTTHFHISVPLQEVLKDVKDFLTTPASANEFVILDFLHFYGFNESHTMKRFVEELQALEEFYIPTTVSLTTPLCNLWQSTRRIFLVVRPYVEYPYARLRSVALKSIWVNQMELNDLLDRLEELMTRDLEDVSIGGVPSKMYVTQAIGTPRNNDFAVAACCGACPGSHPDLYSAAKHKNPHLLKWFYDLNVNGVMRGERVTIRRGNNTHGNILLLDFVQEGTCTVKGVDKPMNAVALCVHLNTNQTARS |
| Enzyme Length | 358 |
| Uniprot Accession Number | P09194 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol = 6-(alpha-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate + a 1,2-diacyl-sn-glycerol; Xref=Rhea:RHEA:14333, ChEBI:CHEBI:17815, ChEBI:CHEBI:57997, ChEBI:CHEBI:58891; EC=4.6.1.14; |
| DNA Binding | |
| EC Number | 4.6.1.14 |
| Enzyme Function | FUNCTION: By hydrolysis of the attached glycolipid, releases soluble variant surface glycoprotein containing phosphoinositol from the cell wall of T.brucei after cell lysis. It also cleaves similar membrane anchors on some mammalian proteins. VSG lipase may play a role in processes such as parasite differentiation or antigenic variation. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Domain (1); Sequence conflict (5) |
| Keywords | Direct protein sequencing;Lyase;Membrane |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein. |
| Modified Residue | |
| Post Translational Modification | PTM: The N-terminus is blocked. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 40,663 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:14333 |
| Cross Reference Brenda |