| IED ID | IndEnz0005000726 |
| Enzyme Type ID | lipase000726 |
| Protein Name |
Secreted effector protein SseJ EC 3.-.-.- Salmonella-translocated effector J STE J |
| Gene Name | sseJ STM1631 |
| Organism | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella Salmonella enterica (Salmonella choleraesuis) Salmonella enterica I Salmonella typhimurium Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
| Enzyme Sequence | MPLSVGQGYFTSSISSEKFNAIKESARLPELSLWEKIKAYFFTTHHAEALECIFNLYHHQELNLTPVQVRGAYIKLRALASQGCKEQFIIESQEHADKLIIKDDNGENILSIEVECHPEAFGLAKEINKSHPKPKNISLGDITRLVFFGDSLSDSLGRMFEKTHHILPSYGQYFGGRFTNGFTWTEFLSSPHFLGKEMLNFAEGGSTSASYSCFNCIGDFVSNTDRQVASYTPSHQDLAIFLLGANDYMTLHKDNVIMVVEQQIDDIEKIISGGVNNVLVMGIPDLSLTPYGKHSDEKRKLKDESIAHNALLKTNVEELKEKYPQHKICYYETADAFKVIMEAASNIGYDTENPYTHHGYVHVPGAKDPQLDICPQYVFNDLVHPTQEVHHCFAIMLESFIAHHYSTE |
| Enzyme Length | 408 |
| Uniprot Accession Number | Q9FD10 |
| Absorption | |
| Active Site | ACT_SITE 151; /note=Nucleophile; /evidence=ECO:0000305; ACT_SITE 381; /evidence=ECO:0000250; ACT_SITE 384; /evidence=ECO:0000305 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.-.-.- |
| Enzyme Function | FUNCTION: Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is required for endosomal tubulation and negatively regulates the formation of Salmonella-induced filaments (Sifs) in epithelial cells. Has both deacylase and esterification activities in vitro, but esterification is probably the dominant activity in host cells. Significantly contributes to cholesterol esterification, which reduces cellular cholesterol in cells and abrogates the ability of SifA to associate with cholesterol and LAMP-1 vesicles. {ECO:0000269|PubMed:16177296, ECO:0000269|PubMed:18333886}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Mutagenesis (4) |
| Keywords | Host cytoplasm;Hydrolase;Reference proteome;Secreted;Virulence |
| Interact With | P61586 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. Host cytoplasm. Note=Secreted via type III secretion system 2 (SPI-2 TTSS), and delivered into the host cytoplasm. Localizes to the Salmonella-containing vacuole (SCV) at early time points following invasion and subsequently traffics away from the SCV along Salmonella-induced filaments (Sifs) in epithelial cells. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 18757801; 19887681; 21138562; 28235057; 29921673; |
| Motif | |
| Gene Encoded By | |
| Mass | 46,077 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |