| IED ID | IndEnz0005000727 |
| Enzyme Type ID | lipase000727 |
| Protein Name |
Phenmedipham hydrolase EC 3.1.1.- Phenylcarbamate hydrolase |
| Gene Name | pcd |
| Organism | Pseudarthrobacter oxydans (Arthrobacter oxydans) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Micrococcales Micrococcaceae Pseudarthrobacter Pseudarthrobacter oxydans (Arthrobacter oxydans) |
| Enzyme Sequence | MITRPIAHTTAGDLGGCLEDGLYVFRGVPYAEPPVGDLRWRAARPHAGWTGVRDASAYGPSAPQPVEPGGSPILGTHGDPPFDEDCLTLNLWTPNLDGGSRPVLVWIHGGGLLTGSGNLPNYATDTFARDGDLVGISINYRLGPLGFLAGMGDENVWLTDQVEALRWIADNVAAFGGDPNRITLVGQSGGAYSIAALAQHPVARQLFHRAILQSPPFGMQPHTVEESTARTKALARHLGHDDIEALRHEPWERLIQGTIGVLMEHTKFGEWPLAFYPVFDEATIPRHPIESIIDSDIEIIIGWTRDEGTFPFAFDPQVSQADRDQVESWLQKRFGDHAASAYEAHAGDGTSPWTVIANVVGDELFHSAGYRVADERATRRPVRAYQFDVVSPLSDGALGAVHCIEMPFTFANLDRWTGKPFVDGLDPDVVARVTNVLHQAWIAFVRTGDPTHDQLPVWPTFRADDPAVLVVGDEGAEVARDLARPDHVSVRTL |
| Enzyme Length | 493 |
| Uniprot Accession Number | Q01470 |
| Absorption | |
| Active Site | ACT_SITE 188; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 307; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 402; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: May degrade the phenylcarbamate herbicides phenmedipham and desmedipham cometabolically by hydrolyzing their central carbamate linkages. Conveys resistance to the herbicide phenmedipham. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1) |
| Keywords | Direct protein sequencing;Herbicide resistance;Hydrolase;Plasmid;Serine esterase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | Plasmid pHP52 |
| Mass | 53,728 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |