| IED ID | IndEnz0005000731 |
| Enzyme Type ID | lipase000731 |
| Protein Name |
Patatin-like protein 2 AtPLP2 EC 3.1.1.- Patatin-related phospholipase A IIalpha pPLAIIa Phospholipase A IIA AtPLAIIA |
| Gene Name | PLP2 At2g26560 T9J22.23 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MQMDSPKSPLQPPTYGNLVTILSIDGGGIRGLIPAVILGFLESELQKLDGEEARLADYFDVIAGTSTGGLVTAMLTAPNKEGRPLFAASEIKDFYLEQCPKIFPQDHFPFSAAKKLVKSLTGPKYDGKYLHQLIHAKLGDTKLSQTLTNVVIPTFDIKHLQPTIFSSYEVKNHPLKDATLADIAISTSAAPTYLPAHFFKVEDLNGNAKEYNLIDGGVAANNPALLAIGEVTNEISGGSSDFFPIRPNDYGRFLVLSLGTGNHKAEEKFNAKEVAGWGLLNWLTHDNSTPIIDAFSQASSDMVDFHLSAVFRALHSEANYIRIQDDTLTGDAASVDIATVENLDILAKTGDELLKKPVARVNLDSGCNENAYETTNEHALIKLAGILSKEKKIRDIRSPHAKAPIRI |
| Enzyme Length | 407 |
| Uniprot Accession Number | O48723 |
| Absorption | |
| Active Site | ACT_SITE 66; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 215; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Possesses non-specific lipolytic acyl hydrolase (LAH) activity. Catalyzes the hydrolysis of the galactolipids monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), and less efficiently the phoshpolipids phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidic acid (PA), phosphatidylserine (PS) and phosphatidylinositol (PI). Favors the release of fatty acid at the sn-1 position for PC or PE and the sn-2 position for PG, PA, PS and PI. Negatively affects disease resistance to the necrotic fungal pathogen Botrytis cinerea and the avirulent bacteria Pseudomonas syringae by promoting cell death and reducing the efficiency of the hypersensitive response, respectively. However, PLP2 contributes to resistance to cucumber mosaic virus (CMV), an obligate parasite inducing hypersensitive response. May negatively regulate oxylipin production, possibly via participating in membrane repair that includes removal of oxidatively modified lipids. {ECO:0000269|PubMed:16297072, ECO:0000269|PubMed:19271961, ECO:0000269|PubMed:22259021}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (1); Erroneous initiation (1); Modified residue (1); Motif (3); Sequence conflict (1) |
| Keywords | Cytoplasm;Hydrolase;Lipid degradation;Lipid metabolism;Phosphoprotein;Plant defense;Reference proteome |
| Interact With | Q39070; Itself; Q9FHZ1 |
| Induction | INDUCTION: By infection with the fungal pathogens B.cinerea and A.brassicicola, and avirulent and virulent strains of P.syringae pv tomato DC3000 (at protein level). Induced by ethylene and copper. {ECO:0000269|PubMed:14634163, ECO:0000269|PubMed:16297072}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12226489, ECO:0000269|PubMed:16297072}. |
| Modified Residue | MOD_RES 398; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:O23179 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11752382; 14617064; 14722088; 15118858; 15130548; 15215506; 15516340; 15531708; 15713015; 15824099; 16005291; 16236154; 16630346; 16766691; 16776300; 17075075; 17194768; 17220200; 17411438; 17475618; 18467450; 18650403; 18684332; 18702669; 21398256; 21447788; 21798944; 23505340; 23858430; 29666162; |
| Motif | MOTIF 26..31; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 64..68; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 215..217; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Gene Encoded By | |
| Mass | 44,239 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.1.1.23; |