Detail Information for IndEnz0005000735
IED ID IndEnz0005000735
Enzyme Type ID lipase000735
Protein Name Phospholipase A1-Ialpha2, chloroplastic
EC 3.1.1.-
DAD1-like lipase 5
Gene Name DALL5 At2g31690 T9H9.21
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MALIQNPNMKHAPFLRNRSPQQTLFIPYTLSLPISYQNPKRLKTANSSSSSSLLAPVILNSPVASSSPPPIYCAPKFPCSSGAATVPLSRVWREIQGCNNWKDLIEPLNPLLQQEITRYGNLVSTCYKAFDLDPNSKRYLNCKYGKQTLLKETEIDQPEDYQVTKYIYATPDININISPIQNEMNRRARWVGYVAASSDDSVKRLGRRDIVVTFRGTVTNPEWLANFMSSLTPARFHPHNPRLDVKVESGFLSLYTSDESESKFGLESCRQQLLSEISRLMNKYKGEEMSITLAGHSMGSSLAQLLAYDIAELGLNRRIGKGDIPVTVFSFAGPRVGNLEFKKRCEELGVKVLRITNVNDPVTKLPGVLFNENFRVLGGFYELPWSCSCYVHVGVELTLDFFDVQNISCVHDLQTYIDLLNQRRTNSRSVDSDEDEDSDNVALEFLKTNGEKMMFLKRQRMMYWSNAVDLLFSFSNHMSYCNIF
Enzyme Length 484
Uniprot Accession Number Q9SIN9
Absorption
Active Site ACT_SITE 297; /note=Acyl-ester intermediate; /evidence=ECO:0000250|UniProtKB:Q948R1; ACT_SITE 360; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q948R1; ACT_SITE 411; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q948R1
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=1,2-diacyl-3-O-[alpha-D-galactosyl-(1->6)-beta-D-galactosyl]-sn-glycerol + H2O = a fatty acid + acyl-3-O-[alpha-D-galactosyl-(1->6)-beta-D-galactosyl]-sn-glycerol + H(+); Xref=Rhea:RHEA:48372, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28396, ChEBI:CHEBI:28868, ChEBI:CHEBI:90310; Evidence={ECO:0000269|PubMed:19527719};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:48373; Evidence={ECO:0000269|PubMed:19527719}; CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + H2O = a fatty acid + an acyl-3-O-(beta-D-galactosyl)-sn-glycerol + H(+); Xref=Rhea:RHEA:57084, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17615, ChEBI:CHEBI:28868, ChEBI:CHEBI:141434; Evidence={ECO:0000269|PubMed:19527719};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57085; Evidence={ECO:0000269|PubMed:19527719};
DNA Binding
EC Number 3.1.1.-
Enzyme Function FUNCTION: Acylhydrolase that catalyzes the hydrolysis of phosphatidylcholine at the sn-1 position. Has a strong galactolipase activity toward monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG). Low triacylglycerol (TAG) lipase activity. Plays a role in plant growth and in leaf senescence. {ECO:0000269|PubMed:17259290}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Motif (1); Transit peptide (1)
Keywords Chloroplast;Hydrolase;Lipid degradation;Lipid metabolism;Plastid;Reference proteome;Transit peptide
Interact With
Induction INDUCTION: Induced by wounding (PubMed:24430866). Not induced by wounding (PubMed:18267087). {ECO:0000269|PubMed:18267087, ECO:0000269|PubMed:24430866}.
Subcellular Location SUBCELLULAR LOCATION: Plastid, chloroplast, plastoglobule {ECO:0000269|PubMed:17259290, ECO:0000269|PubMed:19527719}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 14576160; 16510518; 23505340;
Motif MOTIF 295..299; /note=GXSXG; /evidence=ECO:0000250|UniProtKB:Q948R1
Gene Encoded By
Mass 54,924
Kinetics
Metal Binding
Rhea ID RHEA:48372; RHEA:48373; RHEA:57084; RHEA:57085
Cross Reference Brenda