Detail Information for IndEnz0005000773
IED ID IndEnz0005000773
Enzyme Type ID lipase000773
Protein Name Phospholipase A1 verutoxin-2b
VT-2b
EC 3.1.1.32
EC 3.1.1.5
Fragment
Gene Name
Organism Vespa velutina (Asian yellow-legged hornet)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Hymenoptera Apocrita (wasps ants and bees) Aculeata Vespoidea Vespidae (wasps) Vespinae (hornets and yellowjackets) Vespa Vespa velutina (Asian yellow-legged hornet)
Enzyme Sequence FNPCPYSDDTVKMIILTRENKKHDF
Enzyme Length 25
Uniprot Accession Number P0DMB8
Absorption
Active Site
Activity Regulation ACTIVITY REGULATION: Activity is maximal in the presence of calcium. However, unlike phospholipases A2 whose catalytic activity is strictly calcium-dependent, this enzyme shows considerable catalytic activity on phosphatidylcholine emulsified in calcium free solution. {ECO:0000269|PubMed:10484737}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:18689, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:57643, ChEBI:CHEBI:57875; EC=3.1.1.32; Evidence={ECO:0000269|PubMed:10484737};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:18690; Evidence={ECO:0000269|PubMed:10484737}; CATALYTIC ACTIVITY: Reaction=1-(9Z-octadecenoyl)-2-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = (9Z)-octadecenoate + 2-hexadecanoyl-sn-glycero-3-phosphocholine + H(+); Xref=Rhea:RHEA:38787, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:74667, ChEBI:CHEBI:76078; Evidence={ECO:0000269|PubMed:10484737};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38788; Evidence={ECO:0000269|PubMed:10484737}; CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; Evidence={ECO:0000269|PubMed:10484737};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15178; Evidence={ECO:0000269|PubMed:10484737};
DNA Binding
EC Number 3.1.1.32; 3.1.1.5
Enzyme Function FUNCTION: Catalyzes the hydrolysis of glycerophospholipids such as phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine) and has a moderate activity to hydrolyze lysoglycerophospholipids such as lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine), but is unable to hydrolyze sphingomyelin. In addition to acting as allergens, it possesses a potent hemolytic activity on red blood cells of mice (99.3% of hemolysis at 3.0 ug/ml). {ECO:0000269|PubMed:10484737}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Phospholipid metabolism. {ECO:0000305|PubMed:10484737}.
nucleotide Binding
Features Chain (1); Non-terminal residue (1)
Keywords Allergen;Cytolysis;Direct protein sequencing;Disulfide bond;Hemolysis;Hydrolase;Lipid degradation;Lipid metabolism;Secreted
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10484737}.
Modified Residue
Post Translational Modification PTM: Contains six disulfide bonds. {ECO:0000250}.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 3,012
Kinetics
Metal Binding
Rhea ID RHEA:18689; RHEA:18690; RHEA:38787; RHEA:38788; RHEA:15177; RHEA:15178
Cross Reference Brenda