| IED ID | IndEnz0005000796 |
| Enzyme Type ID | lipase000796 |
| Protein Name |
Phospholipase A1 EC 3.1.1.32 Venom allergen 1 |
| Gene Name | |
| Organism | Dinoponera quadriceps (South American ant) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Hymenoptera Apocrita (wasps ants and bees) Aculeata Formicoidea Formicidae Ponerinae Ponerini Dinoponera Dinoponera quadriceps (South American ant) |
| Enzyme Sequence | MKFITAILVIFCVYLLSTAGDSKILPLKKLPSKIFGHLKSHVDNTVKKPLKVFGHLKSHVENSVGPLRMNKLTPNCIFGVKSMSMVLFTKNIPDGKYISLDSDLGRDLDLTKTIYFTAHGFISNVNHSLSNRLSRALVEKDYTVFSLDWSDAACTTGGLPLVKLLGYPSAVQNTREIGNLMADYVMSLIDHGASLRNMAFIGHSLGSHVCGFASKKIYESGYGKVPLLFAADPAQPLFQLKQCPDRLCDTDAKLVITLHTSQIGLGYPIGGLDLYFNGGFVQPKCHLDITCAHIRSVLYLINMVEKKCSFPGIPATYKQILNPFSKFPYPNSKTTDCFVMDDSIFNPRRKSLQNLAGGIYYMFVDPDTFCTRKNFNCQR |
| Enzyme Length | 379 |
| Uniprot Accession Number | P0DSI2 |
| Absorption | |
| Active Site | ACT_SITE 204; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:A0A0M3KKW3; ACT_SITE 232; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037; ACT_SITE 293; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:18689, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:57643, ChEBI:CHEBI:57875; EC=3.1.1.32; Evidence={ECO:0000250|UniProtKB:Q68KK0}; |
| DNA Binding | |
| EC Number | 3.1.1.32 |
| Enzyme Function | FUNCTION: Catalyzes the hydrolysis of phosphatidylcholine with phospholipase A1 activity. Shows hemolytic activity. {ECO:0000250|UniProtKB:Q68KK0}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Glycosylation (1); Propeptide (1); Signal peptide (1) |
| Keywords | Allergen;Cytolysis;Disulfide bond;Glycoprotein;Hemolysis;Hydrolase;Lipid degradation;Lipid metabolism;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:24498135}. |
| Modified Residue | |
| Post Translational Modification | PTM: Contains five disulfide bonds. {ECO:0000305}. |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 42,135 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:18689 |
| Cross Reference Brenda |