| IED ID | IndEnz0005000802 |
| Enzyme Type ID | lipase000802 |
| Protein Name |
Phospholipase A1 EC 3.1.1.32 Allergen Sol i I Venom allergen 1 Venom allergen I allergen Sol i 1 |
| Gene Name | |
| Organism | Solenopsis invicta (Red imported fire ant) (Solenopsis wagneri) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Hymenoptera Apocrita (wasps ants and bees) Aculeata Formicoidea Formicidae Myrmicinae Solenopsidini Solenopsis Solenopsis invicta (Red imported fire ant) (Solenopsis wagneri) |
| Enzyme Sequence | MRKFAAIFVVFFVQCTHLYSLAQARAEPDPGVVEYLKQSCVYGNSSYINVYLYNSRFQGKNLGNQQSCQDINASLPVVFITHGFTSSAQVSTFKDLANAFVQKGHTAFIVDWSEAACTDGLPGVQFAEYNAAASNTYDIGQLMAKYTVDLMNKCKIPLNNIQYVGHSLGSHVCGFAAKHVKKLINKTMPYILALDPADPSFGSNKCGERICKSDAKRIVVFKTSILGIGENIIGHLLIVFDGGKSQPACSWYDVPCSHSESIVYATGMVSGRCQHLAVPWTAQQRINPIQWKFWRVFTSNIPAYPTSDTTNCVVLNTNVFKNDNTFEGEYHAFPDCARNLFKCRQQ |
| Enzyme Length | 346 |
| Uniprot Accession Number | Q68KK0 |
| Absorption | |
| Active Site | ACT_SITE 167; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:A0A0M3KKW3; ACT_SITE 195; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037; ACT_SITE 258; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:18689, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:57643, ChEBI:CHEBI:57875; EC=3.1.1.32; Evidence={ECO:0000305|PubMed:15753912}; |
| DNA Binding | |
| EC Number | 3.1.1.32 |
| Enzyme Function | FUNCTION: Catalyzes the hydrolysis of phosphatidylcholine with phospholipase A1 activity. Shows hemolytic activity (Probable). {ECO:0000305|PubMed:15753912}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Glycosylation (3); Propeptide (1); Sequence conflict (8); Signal peptide (1) |
| Keywords | Allergen;Cytolysis;Direct protein sequencing;Disulfide bond;Glycoprotein;Hemolysis;Hydrolase;Lipid degradation;Lipid metabolism;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15753912, ECO:0000269|PubMed:8588684}. |
| Modified Residue | |
| Post Translational Modification | PTM: Contains six disulfide bonds. {ECO:0000250}.; PTM: N-glycosylated; contains mannose. {ECO:0000269|PubMed:15753912}. |
| Signal Peptide | SIGNAL 1..26; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 38,417 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:18689 |
| Cross Reference Brenda |