IED Industry Enzyme Database

Detail Information for IndEnz0005000812
IED ID IndEnz0005000812
Enzyme Type ID lipase000812
Protein Name Phospholipase A2 group XV
1-O-acylceramide synthase
ACS
LCAT-like lysophospholipase
LLPL
EC 3.1.1.5
Lysophospholipase 3
Lysosomal phospholipase A and acyltransferase
EC 2.3.1.-
EC 3.1.1.32
EC 3.1.1.4
Lysosomal phospholipase A2
LPLA2
Gene Name PLA2G15 LYPLA3 UNQ341/PRO540
Organism Homo sapiens (Human)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human)
Enzyme Sequence MGLHLRPYRVGLLPDGLLFLLLLLMLLADPALPAGRHPPVVLVPGDLGNQLEAKLDKPTVVHYLCSKKTESYFTIWLNLELLLPVIIDCWIDNIRLVYNKTSRATQFPDGVDVRVPGFGKTFSLEFLDPSKSSVGSYFHTMVESLVGWGYTRGEDVRGAPYDWRRAPNENGPYFLALREMIEEMYQLYGGPVVLVAHSMGNMYTLYFLQRQPQAWKDKYIRAFVSLGAPWGGVAKTLRVLASGDNNRIPVIGPLKIREQQRSAVSTSWLLPYNYTWSPEKVFVQTPTINYTLRDYRKFFQDIGFEDGWLMRQDTEGLVEATMPPGVQLHCLYGTGVPTPDSFYYESFPDRDPKICFGDGDGTVNLKSALQCQAWQSRQEHQVLLQELPGSEHIEMLANATTLAYLKRVLLGP
Enzyme Length 412
Uniprot Accession Number Q8NCC3
Absorption
Active Site ACT_SITE 198; /note="Acyl-ester intermediate"; /evidence="ECO:0000255, ECO:0000269|PubMed:25727495, ECO:0007744|PDB:4X97"; ACT_SITE 360; /note="Charge relay system"; /evidence="ECO:0000305|PubMed:25727495"; ACT_SITE 392; /note="Charge relay system"; /evidence="ECO:0000305|PubMed:25727495"
Activity Regulation ACTIVITY REGULATION: Inhibited by zinc ions at neutral pH. Zinc ions in plasma may keep the enzyme from hydrolyzing inappropriate substrates. {ECO:0000269|PubMed:30830753}.
Binding Site BINDING 46; /note="Substrate; via amide nitrogen"; /evidence="ECO:0000305|PubMed:25727495, ECO:0007744|PDB:4X97"; BINDING 199; /note="Substrate; via amide nitrogen"; /evidence="ECO:0000305|PubMed:25727495, ECO:0007744|PDB:4X95"
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:18689, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:57643, ChEBI:CHEBI:57875; EC=3.1.1.32; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:18690; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H(+) + hexadecanoate; Xref=Rhea:RHEA:38783, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:73001, ChEBI:CHEBI:76071; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38784; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-glutaroyl-sn-glycero-3-phosphocholine + H2O = 2-glutaroyl-sn-glycero-3-phosphocholine + H(+) + hexadecanoate; Xref=Rhea:RHEA:62480, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:77756, ChEBI:CHEBI:145781; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62481; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-nonadioyl-sn-glycero-3-phosphocholine + H2O = 2-nonadioyl-sn-glycero-3-phosphocholine + H(+) + hexadecanoate; Xref=Rhea:RHEA:62464, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:78207, ChEBI:CHEBI:145780; Evidence={ECO:0000269|PubMed:30830753};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62465; Evidence={ECO:0000305|PubMed:30830753}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(5-oxopentanoyl)-sn-glycero-3-phosphocholine + H2O = 2-(5-oxopentanoyl)-sn-glycero-3-phosphocholine + H(+) + hexadecanoate; Xref=Rhea:RHEA:62484, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:77890, ChEBI:CHEBI:145782; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62485; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9-oxononanoyl)-sn-glycero-3-phosphocholine + H2O = 2-(9-oxononanoyl)-sn-glycero-3-phosphocholine + H(+) + hexadecanoate; Xref=Rhea:RHEA:62488, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:61042, ChEBI:CHEBI:145783; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62489; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:15801, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:57643, ChEBI:CHEBI:58168; EC=3.1.1.4; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15802; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = (9Z)-octadecenoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+); Xref=Rhea:RHEA:38779, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:72998, ChEBI:CHEBI:73001; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38780; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; Evidence={ECO:0000269|PubMed:10092508};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15178; Evidence={ECO:0000305|PubMed:10092508}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = H(+) + hexadecanoate + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:40435, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:72998; Evidence={ECO:0000269|PubMed:10092508};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40436; Evidence={ECO:0000305|PubMed:10092508}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:38827, ChEBI:CHEBI:46979, ChEBI:CHEBI:73007, ChEBI:CHEBI:76077, ChEBI:CHEBI:76088; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38828; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:38823, ChEBI:CHEBI:46979, ChEBI:CHEBI:73004, ChEBI:CHEBI:73007, ChEBI:CHEBI:76054; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38824; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z,12Z)-octadecadienoyl-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:38831, ChEBI:CHEBI:46979, ChEBI:CHEBI:73008, ChEBI:CHEBI:76077, ChEBI:CHEBI:76090; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38832; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine + N-(acetyl)-sphing-4-enine = 1-(9Z,12Z-octadecadienoyl)-N-acetylsphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:38835, ChEBI:CHEBI:46979, ChEBI:CHEBI:73004, ChEBI:CHEBI:73008, ChEBI:CHEBI:76086; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38836; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:38843, ChEBI:CHEBI:46979, ChEBI:CHEBI:73009, ChEBI:CHEBI:76077, ChEBI:CHEBI:76091; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38844; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + N-(acetyl)-sphing-4-enine = 1-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-N-(acetyl)-sphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:38839, ChEBI:CHEBI:46979, ChEBI:CHEBI:73004, ChEBI:CHEBI:73009, ChEBI:CHEBI:76080; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38840; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38759, ChEBI:CHEBI:46979, ChEBI:CHEBI:73001, ChEBI:CHEBI:76071, ChEBI:CHEBI:76077; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38760; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38755, ChEBI:CHEBI:46979, ChEBI:CHEBI:72998, ChEBI:CHEBI:73001, ChEBI:CHEBI:76054; Evidence={ECO:0000269|PubMed:29724779};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38756; Evidence={ECO:0000305|PubMed:29724779}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38811, ChEBI:CHEBI:46979, ChEBI:CHEBI:73002, ChEBI:CHEBI:76077, ChEBI:CHEBI:76084; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38812; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z,12Z-octadecadienoyl)-N-acetylsphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38807, ChEBI:CHEBI:46979, ChEBI:CHEBI:72998, ChEBI:CHEBI:73002, ChEBI:CHEBI:76086; Evidence={ECO:0000269|PubMed:29724779};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38808; Evidence={ECO:0000305|PubMed:29724779}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38775, ChEBI:CHEBI:46979, ChEBI:CHEBI:73003, ChEBI:CHEBI:76077, ChEBI:CHEBI:76079; Evidence={ECO:0000269|PubMed:29724779};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38776; Evidence={ECO:0000305|PubMed:29724779}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-N-(acetyl)-sphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38771, ChEBI:CHEBI:46979, ChEBI:CHEBI:72998, ChEBI:CHEBI:73003, ChEBI:CHEBI:76080; Evidence={ECO:0000269|PubMed:29724779};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38772; Evidence={ECO:0000305|PubMed:29724779}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38815, ChEBI:CHEBI:46979, ChEBI:CHEBI:74963, ChEBI:CHEBI:76077, ChEBI:CHEBI:76085; Evidence={ECO:0000269|PubMed:29724779};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38816; Evidence={ECO:0000305|PubMed:29724779}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-N-(acetyl)-sphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38819, ChEBI:CHEBI:46979, ChEBI:CHEBI:72998, ChEBI:CHEBI:74963, ChEBI:CHEBI:76087; Evidence={ECO:0000269|PubMed:29724779};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38820; Evidence={ECO:0000305|PubMed:29724779}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-nonadioyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-nonadioyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:62472, ChEBI:CHEBI:46979, ChEBI:CHEBI:76077, ChEBI:CHEBI:78207, ChEBI:CHEBI:145780; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62473; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-octadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38799, ChEBI:CHEBI:46979, ChEBI:CHEBI:75034, ChEBI:CHEBI:76071, ChEBI:CHEBI:76074; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38800; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 1-octadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38795, ChEBI:CHEBI:46979, ChEBI:CHEBI:73858, ChEBI:CHEBI:75034, ChEBI:CHEBI:76054; Evidence={ECO:0000269|PubMed:29724779};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38796; Evidence={ECO:0000305|PubMed:29724779}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z,12Z)-octadecadienoyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z,12Z-octadecadienoyl)-N-acetylsphing-4-enine + 1-octadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:57108, ChEBI:CHEBI:46979, ChEBI:CHEBI:73858, ChEBI:CHEBI:76086, ChEBI:CHEBI:84822; Evidence={ECO:0000269|PubMed:29724779};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57109; Evidence={ECO:0000305|PubMed:29724779}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-octadecanoyl-N-(acetyl)-sphing-4-enine + 2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:57120, ChEBI:CHEBI:46979, ChEBI:CHEBI:74965, ChEBI:CHEBI:76074, ChEBI:CHEBI:76079; Evidence={ECO:0000269|PubMed:29724779};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57121; Evidence={ECO:0000305|PubMed:29724779}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-N-(acetyl)-sphing-4-enine + 1-octadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:57116, ChEBI:CHEBI:46979, ChEBI:CHEBI:73858, ChEBI:CHEBI:74965, ChEBI:CHEBI:76080; Evidence={ECO:0000269|PubMed:29724779};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57117; Evidence={ECO:0000305|PubMed:29724779}; CATALYTIC ACTIVITY: Reaction=1-(9Z-octadecenoyl)-2-hexadecanoyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 2-hexadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38767, ChEBI:CHEBI:46979, ChEBI:CHEBI:74667, ChEBI:CHEBI:76054, ChEBI:CHEBI:76078; Evidence={ECO:0000269|PubMed:29724779};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38768; Evidence={ECO:0000305|PubMed:29724779}; CATALYTIC ACTIVITY: Reaction=1-(9Z-octadecenoyl)-2-hexadecanoyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-hexadecanoyl-N-(acetyl)-sphing-4-enine; Xref=Rhea:RHEA:38763, ChEBI:CHEBI:28610, ChEBI:CHEBI:46979, ChEBI:CHEBI:74667, ChEBI:CHEBI:76077; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38764; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1-(9Z)-octadecenoyl-2-octadecanoyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 2-octadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38791, ChEBI:CHEBI:46979, ChEBI:CHEBI:76054, ChEBI:CHEBI:76073, ChEBI:CHEBI:76076; Evidence={ECO:0000269|PubMed:29724779};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38792; Evidence={ECO:0000305|PubMed:29724779}; CATALYTIC ACTIVITY: Reaction=1-(9Z)-octadecenoyl-2-octadecanoyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-octadecanoyl-N-(acetyl)-sphing-4-enine; Xref=Rhea:RHEA:38803, ChEBI:CHEBI:28610, ChEBI:CHEBI:46979, ChEBI:CHEBI:76073, ChEBI:CHEBI:76074; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38804; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38703, ChEBI:CHEBI:28610, ChEBI:CHEBI:46979, ChEBI:CHEBI:74669, ChEBI:CHEBI:76054; Evidence={ECO:0000269|PubMed:11790796, ECO:0000269|PubMed:20410020, ECO:0000269|PubMed:23958596, ECO:0000269|PubMed:25727495, ECO:0000269|PubMed:29724779};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38704; Evidence={ECO:0000305|PubMed:11790796, ECO:0000305|PubMed:20410020, ECO:0000305|PubMed:23958596, ECO:0000305|PubMed:25727495, ECO:0000305|PubMed:29724779}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + N-(acetyl)-sphing-4-enine = 1-octadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine; Xref=Rhea:RHEA:57140, ChEBI:CHEBI:46979, ChEBI:CHEBI:76074, ChEBI:CHEBI:77342, ChEBI:CHEBI:78260; Evidence={ECO:0000269|PubMed:29724779};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57141; Evidence={ECO:0000305|PubMed:29724779}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 1-octadecanoyl-sn-glycero-3-phosphoserine; Xref=Rhea:RHEA:57136, ChEBI:CHEBI:46979, ChEBI:CHEBI:76054, ChEBI:CHEBI:78260, ChEBI:CHEBI:84467; Evidence={ECO:0000269|PubMed:29724779};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57137; Evidence={ECO:0000305|PubMed:29724779}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + N-(acetyl)-sphing-4-enine = 1-octadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol); Xref=Rhea:RHEA:57144, ChEBI:CHEBI:46979, ChEBI:CHEBI:72845, ChEBI:CHEBI:76074, ChEBI:CHEBI:141490; Evidence={ECO:0000269|PubMed:29724779};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57145; Evidence={ECO:0000305|PubMed:29724779}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 1-octadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol); Xref=Rhea:RHEA:57148, ChEBI:CHEBI:46979, ChEBI:CHEBI:72827, ChEBI:CHEBI:72845, ChEBI:CHEBI:76054; Evidence={ECO:0000269|PubMed:29724779};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57149; Evidence={ECO:0000305|PubMed:29724779}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 2-[(5Z,8Z,11Z,14Z)-eicosatetraenoylamino]ethyl (9Z)-octadecenoate; Xref=Rhea:RHEA:38751, ChEBI:CHEBI:2700, ChEBI:CHEBI:74669, ChEBI:CHEBI:76070, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38752; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(9Z-octadecenoyl) ethanolamine = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 2-[(9Z)-octadecenoylamino]ethyl (9Z)-octadecenoate; Xref=Rhea:RHEA:38747, ChEBI:CHEBI:71466, ChEBI:CHEBI:74669, ChEBI:CHEBI:76068, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38748; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 3-(9Z-octadecenoyl)-sn-glycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1,3-di-(9Z-octadecenoyl)-glycerol; Xref=Rhea:RHEA:38743, ChEBI:CHEBI:74669, ChEBI:CHEBI:75735, ChEBI:CHEBI:75938, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38744; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-(9Z-octadecenoyl)-sn-glycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1,3-di-(9Z-octadecenoyl)-glycerol; Xref=Rhea:RHEA:38739, ChEBI:CHEBI:74669, ChEBI:CHEBI:75735, ChEBI:CHEBI:75757, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38740; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 2-hexadecanoylglycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-(9Z)-octadecenoyl-2-hexadecanoylglycerol; Xref=Rhea:RHEA:38735, ChEBI:CHEBI:74669, ChEBI:CHEBI:75455, ChEBI:CHEBI:76065, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38736; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 3-hexadecanoyl-sn-glycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol; Xref=Rhea:RHEA:38731, ChEBI:CHEBI:64757, ChEBI:CHEBI:74669, ChEBI:CHEBI:75867, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38732; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = (9Z)-octadecenoate + (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H(+); Xref=Rhea:RHEA:38699, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:74669, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38700; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-O-hexadecyl-2-acetyl-sn-glycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-O-hexadecyl-2-acetyl-3-(9Z)-octadecenoyl-sn-glycerol; Xref=Rhea:RHEA:38707, ChEBI:CHEBI:74669, ChEBI:CHEBI:75936, ChEBI:CHEBI:76055, ChEBI:CHEBI:76083; Evidence={ECO:0000269|PubMed:29724779};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38708; Evidence={ECO:0000305|PubMed:29724779}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-O-hexadecylglycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-O-hexadecyl-3-(9Z)-octadecenoylglycerol; Xref=Rhea:RHEA:38711, ChEBI:CHEBI:74669, ChEBI:CHEBI:76061, ChEBI:CHEBI:76062, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38712; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-O-hexadecyl-2-O-methyl-sn-glycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-O-hexadecyl-2-O-methyl-3-(9Z)-octadecenoyl-sn-glycerol; Xref=Rhea:RHEA:38723, ChEBI:CHEBI:74669, ChEBI:CHEBI:76063, ChEBI:CHEBI:76064, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38724; Evidence={ECO:0000250|UniProtKB:Q8VEB4}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-hexadecanoyl-sn-glycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-hexadecanoyl-3-(9Z)-octadecenoyl-sn-glycerol; Xref=Rhea:RHEA:38727, ChEBI:CHEBI:74669, ChEBI:CHEBI:75542, ChEBI:CHEBI:75868, ChEBI:CHEBI:76083; Evidence={ECO:0000250|UniProtKB:Q8VEB4};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38728; Evidence={ECO:0000250|UniProtKB:Q8VEB4};
DNA Binding
EC Number 3.1.1.5; 2.3.1.-; 3.1.1.32; 3.1.1.4
Enzyme Function FUNCTION: Has dual calcium-independent phospholipase and O-acyltransferase activities with a potential role in glycerophospholipid homeostasis and remodeling of acyl groups of lipophilic alcohols present in acidic cellular compartments (PubMed:10092508, PubMed:11790796, PubMed:20410020, PubMed:23958596, PubMed:29724779, PubMed:25727495). Catalyzes hydrolysis of the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 or A2 activity) and transfer it to the hydroxyl group at the first carbon of lipophilic alcohols (O-acyltransferase activity) (PubMed:10092508, PubMed:11790796, PubMed:20410020, PubMed:23958596, PubMed:29724779, PubMed:25727495). Among preferred fatty acyl donors are phosphatidylcholines, phosphatidylethanolamines, phosphatidylglycerols and phosphatidylserines (PubMed:29724779). Favors sn-2 over sn-1 deacylation of unsaturated fatty acyl groups of phosphatidylcholines and phosphatidylethanolamines (By similarity). Among preferred fatty acyl acceptors are natural lipophilic alcohols including short-chain ceramide N-acetyl-sphingosine (C2 ceramide), alkylacylglycerols, monoacylglycerols, and acylethanolamides such as anandamide and oleoylethanolamide (PubMed:29724779). Selectively hydrolyzes the sn-1 fatty acyl group of truncated oxidized phospholipids and may play a role in detoxification of reactive oxidized phospholipids during oxidative stress (PubMed:30830753). Required for normal phospholipid degradation in alveolar macrophages with potential implications in pulmonary surfactant clearance (By similarity). At neutral pH, hydrolyzes the sn-1 fatty acyl group of the lysophosphatidylcholines (PubMed:10092508). {ECO:0000250|UniProtKB:Q8VEB4, ECO:0000269|PubMed:10092508, ECO:0000269|PubMed:11790796, ECO:0000269|PubMed:20410020, ECO:0000269|PubMed:23958596, ECO:0000269|PubMed:25727495, ECO:0000269|PubMed:29724779, ECO:0000269|PubMed:30830753}.
Temperature Dependency
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4-4.5. {ECO:0000269|PubMed:29724779};
Pathway
nucleotide Binding
Features Active site (3); Alternative sequence (2); Beta strand (17); Binding site (2); Chain (1); Disulfide bond (1); Erroneous initiation (1); Glycosylation (4); Helix (19); Metal binding (4); Mutagenesis (11); Sequence conflict (2); Signal peptide (1); Turn (5)
Keywords 3D-structure;Acyltransferase;Alternative splicing;Direct protein sequencing;Disulfide bond;Fatty acid metabolism;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Lysosome;Membrane;Metal-binding;Reference proteome;Secreted;Signal;Transferase;Zinc
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Lysosome {ECO:0000269|PubMed:23958596}. Secreted {ECO:0000269|PubMed:10092508, ECO:0000269|PubMed:20410020}. Membrane {ECO:0000269|PubMed:25727495}; Peripheral membrane protein {ECO:0000269|PubMed:25727495}.
Modified Residue
Post Translational Modification PTM: N-glycosylated (PubMed:11790796, PubMed:23958596). N-glycosylation is important for maturation of the enzyme and normal subcellular location (PubMed:23958596). {ECO:0000269|PubMed:11790796, ECO:0000269|PubMed:23958596}.
Signal Peptide SIGNAL 1..33; /evidence="ECO:0000269|PubMed:10092508, ECO:0000269|PubMed:15340161"
Structure 3D X-ray crystallography (8)
Cross Reference PDB 4X90; 4X91; 4X92; 4X93; 4X94; 4X95; 4X97; 6MTW;
Mapped Pubmed ID 14754907; 16169070; 18983494; 19461040; 19913121; 20217867; 20628086; 20877624; 27662254; 29330284; 33307060; 9525960;
Motif
Gene Encoded By
Mass 46,658
Kinetics
Metal Binding METAL 198; /note=Zinc; /evidence=ECO:0000269|PubMed:30830753; METAL 340; /note=Zinc; /evidence=ECO:0000269|PubMed:30830753; METAL 355; /note=Zinc; /evidence=ECO:0000269|PubMed:30830753; METAL 392; /note=Zinc; /evidence=ECO:0000269|PubMed:30830753
Rhea ID RHEA:18689; RHEA:18690; RHEA:38783; RHEA:38784; RHEA:62480; RHEA:62481; RHEA:62464; RHEA:62465; RHEA:62484; RHEA:62485; RHEA:62488; RHEA:62489; RHEA:15801; RHEA:15802; RHEA:38779; RHEA:38780; RHEA:15177; RHEA:15178; RHEA:40435; RHEA:40436; RHEA:38827; RHEA:38828; RHEA:38823; RHEA:38824; RHEA:38831; RHEA:38832; RHEA:38835; RHEA:38836; RHEA:38843; RHEA:38844; RHEA:38839; RHEA:38840; RHEA:38759; RHEA:38760; RHEA:38755; RHEA:38756; RHEA:38811; RHEA:38812; RHEA:38807; RHEA:38808; RHEA:38775; RHEA:38776; RHEA:38771; RHEA:38772; RHEA:38815; RHEA:38816; RHEA:38819; RHEA:38820; RHEA:62472; RHEA:62473; RHEA:38799; RHEA:38800; RHEA:38795; RHEA:38796; RHEA:57108; RHEA:57109; RHEA:57120; RHEA:57121; RHEA:57116; RHEA:57117; RHEA:38767; RHEA:38768; RHEA:38763; RHEA:38764; RHEA:38791; RHEA:38792; RHEA:38803; RHEA:38804; RHEA:38703; RHEA:38704; RHEA:57140; RHEA:57141; RHEA:57136; RHEA:57137; RHEA:57144; RHEA:57145; RHEA:57148; RHEA:57149; RHEA:38751; RHEA:38752; RHEA:38747; RHEA:38748; RHEA:38743; RHEA:38744; RHEA:38739; RHEA:38740; RHEA:38735; RHEA:38736; RHEA:38731; RHEA:38732; RHEA:38699; RHEA:38700; RHEA:38707; RHEA:38708; RHEA:38711; RHEA:38712; RHEA:38723; RHEA:38724; RHEA:38727; RHEA:38728
Cross Reference Brenda 3.1.1.4;