IED Industry Enzyme Database

Detail Information for IndEnz0005000813
IED ID IndEnz0005000813
Enzyme Type ID lipase000813
Protein Name Phospholipase A2 group XV
1-O-acylceramide synthase
ACS
LCAT-like lysophospholipase
LLPL
EC 3.1.1.5
Lysophospholipase 3
Lysosomal phospholipase A and acyltransferase
EC 2.3.1.-
EC 3.1.1.32
EC 3.1.1.4
Lysosomal phospholipase A2
LPLA2
Gene Name Pla2g15 Lypla3
Organism Mus musculus (Mouse)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse)
Enzyme Sequence MDRHLCTCRETQLRSGLLLPLFLLMMLADLTLPAQRHPPVVLVPGDLGNQLEAKLDKPKVVHYLCSKKTDSYFTLWLNLELLLPVIIDCWIDNIRLVYNRTSRATQFPDGVDVRVPGFGETFSMEFLDPSKRNVGSYFYTMVESLVGWGYTRGEDVRGAPYDWRRAPNENGPYFLALREMIEEMYQMYGGPVVLVAHSMGNVYMLYFLQRQPQVWKDKYIHAFVSLGAPWGGVAKTLRVLASGDNNRIPVIGPLKIREQQRSAVSTSWLLPYNHTWSHEKVFVYTPTTNYTLRDYHRFFRDIGFEDGWFMRQDTEGLVEAMTPPGVELHCLYGTGVPTPNSFYYESFPDRDPKICFGDGDGTVNLESVLQCQAWQSRQEHRVSLQELPGSEHIEMLANATTLAYLKRVLLEP
Enzyme Length 412
Uniprot Accession Number Q8VEB4
Absorption
Active Site ACT_SITE 198; /note="Acyl-ester intermediate"; /evidence="ECO:0000255, ECO:0000305|PubMed:11790796, ECO:0000305|PubMed:16106046"; ACT_SITE 360; /note="Charge relay system"; /evidence="ECO:0000250|UniProtKB:Q8NCC3, ECO:0000305|PubMed:16106046"; ACT_SITE 392; /note="Charge relay system"; /evidence="ECO:0000250|UniProtKB:Q8NCC3, ECO:0000305|PubMed:16106046"
Activity Regulation ACTIVITY REGULATION: Phospholipase sn-2 versus sn-1 positional specificity is affected by the phospholipid composition of membranes. Phospholipase A2 activity toward 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine (PAPE) is enhanced in the presence of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), which promotes lipid bilayer formation (PubMed:16837646). O-acyltransferase activity is inhibited by antiarrhythmic drug amiodarone (PubMed:27993948). {ECO:0000269|PubMed:16837646, ECO:0000269|PubMed:27993948}.
Binding Site BINDING 46; /note=Substrate; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:Q8NCC3; BINDING 199; /note=Substrate; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:Q8NCC3
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:18689, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:57643, ChEBI:CHEBI:57875; EC=3.1.1.32; Evidence={ECO:0000269|PubMed:27993948};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:18690; Evidence={ECO:0000305|PubMed:27993948}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H(+) + hexadecanoate; Xref=Rhea:RHEA:38783, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:73001, ChEBI:CHEBI:76071; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38784; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-glutaroyl-sn-glycero-3-phosphocholine + H2O = 2-glutaroyl-sn-glycero-3-phosphocholine + H(+) + hexadecanoate; Xref=Rhea:RHEA:62480, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:77756, ChEBI:CHEBI:145781; Evidence={ECO:0000269|PubMed:27993948};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62481; Evidence={ECO:0000305|PubMed:27993948}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-nonadioyl-sn-glycero-3-phosphocholine + H2O = 2-nonadioyl-sn-glycero-3-phosphocholine + H(+) + hexadecanoate; Xref=Rhea:RHEA:62464, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:78207, ChEBI:CHEBI:145780; Evidence={ECO:0000269|PubMed:27993948};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62465; Evidence={ECO:0000305|PubMed:27993948}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(5-oxopentanoyl)-sn-glycero-3-phosphocholine + H2O = 2-(5-oxopentanoyl)-sn-glycero-3-phosphocholine + H(+) + hexadecanoate; Xref=Rhea:RHEA:62484, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:77890, ChEBI:CHEBI:145782; Evidence={ECO:0000269|PubMed:27993948};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62485; Evidence={ECO:0000305|PubMed:27993948}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9-oxononanoyl)-sn-glycero-3-phosphocholine + H2O = 2-(9-oxononanoyl)-sn-glycero-3-phosphocholine + H(+) + hexadecanoate; Xref=Rhea:RHEA:62488, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:61042, ChEBI:CHEBI:145783; Evidence={ECO:0000269|PubMed:27993948};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62489; Evidence={ECO:0000305|PubMed:27993948}; CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:15801, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:57643, ChEBI:CHEBI:58168; EC=3.1.1.4; Evidence={ECO:0000269|PubMed:27993948};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15802; Evidence={ECO:0000305|PubMed:27993948}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = (9Z)-octadecenoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+); Xref=Rhea:RHEA:38779, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:72998, ChEBI:CHEBI:73001; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38780; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15178; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = H(+) + hexadecanoate + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:40435, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:72998; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40436; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:38827, ChEBI:CHEBI:46979, ChEBI:CHEBI:73007, ChEBI:CHEBI:76077, ChEBI:CHEBI:76088; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38828; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:38823, ChEBI:CHEBI:46979, ChEBI:CHEBI:73004, ChEBI:CHEBI:73007, ChEBI:CHEBI:76054; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38824; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z,12Z)-octadecadienoyl-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:38831, ChEBI:CHEBI:46979, ChEBI:CHEBI:73008, ChEBI:CHEBI:76077, ChEBI:CHEBI:76090; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38832; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine + N-(acetyl)-sphing-4-enine = 1-(9Z,12Z-octadecadienoyl)-N-acetylsphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:38835, ChEBI:CHEBI:46979, ChEBI:CHEBI:73004, ChEBI:CHEBI:73008, ChEBI:CHEBI:76086; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38836; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:38843, ChEBI:CHEBI:46979, ChEBI:CHEBI:73009, ChEBI:CHEBI:76077, ChEBI:CHEBI:76091; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38844; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + N-(acetyl)-sphing-4-enine = 1-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-N-(acetyl)-sphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:38839, ChEBI:CHEBI:46979, ChEBI:CHEBI:73004, ChEBI:CHEBI:73009, ChEBI:CHEBI:76080; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38840; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38759, ChEBI:CHEBI:46979, ChEBI:CHEBI:73001, ChEBI:CHEBI:76071, ChEBI:CHEBI:76077; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38760; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38755, ChEBI:CHEBI:46979, ChEBI:CHEBI:72998, ChEBI:CHEBI:73001, ChEBI:CHEBI:76054; Evidence={ECO:0000269|PubMed:16837646, ECO:0000269|PubMed:16880524};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38756; Evidence={ECO:0000305|PubMed:16837646, ECO:0000305|PubMed:16880524}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38811, ChEBI:CHEBI:46979, ChEBI:CHEBI:73002, ChEBI:CHEBI:76077, ChEBI:CHEBI:76084; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38812; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z,12Z-octadecadienoyl)-N-acetylsphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38807, ChEBI:CHEBI:46979, ChEBI:CHEBI:72998, ChEBI:CHEBI:73002, ChEBI:CHEBI:76086; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38808; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38775, ChEBI:CHEBI:46979, ChEBI:CHEBI:73003, ChEBI:CHEBI:76077, ChEBI:CHEBI:76079; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38776; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-N-(acetyl)-sphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38771, ChEBI:CHEBI:46979, ChEBI:CHEBI:72998, ChEBI:CHEBI:73003, ChEBI:CHEBI:76080; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38772; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38815, ChEBI:CHEBI:46979, ChEBI:CHEBI:74963, ChEBI:CHEBI:76077, ChEBI:CHEBI:76085; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38816; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-N-(acetyl)-sphing-4-enine + 1-hexadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38819, ChEBI:CHEBI:46979, ChEBI:CHEBI:72998, ChEBI:CHEBI:74963, ChEBI:CHEBI:76087; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38820; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-nonadioyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-hexadecanoyl-N-(acetyl)-sphing-4-enine + 2-nonadioyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:62472, ChEBI:CHEBI:46979, ChEBI:CHEBI:76077, ChEBI:CHEBI:78207, ChEBI:CHEBI:145780; Evidence={ECO:0000269|PubMed:27993948};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62473; Evidence={ECO:0000305|PubMed:27993948}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-octadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38799, ChEBI:CHEBI:46979, ChEBI:CHEBI:75034, ChEBI:CHEBI:76071, ChEBI:CHEBI:76074; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38800; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 1-octadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38795, ChEBI:CHEBI:46979, ChEBI:CHEBI:73858, ChEBI:CHEBI:75034, ChEBI:CHEBI:76054; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38796; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z,12Z)-octadecadienoyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z,12Z-octadecadienoyl)-N-acetylsphing-4-enine + 1-octadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:57108, ChEBI:CHEBI:46979, ChEBI:CHEBI:73858, ChEBI:CHEBI:76086, ChEBI:CHEBI:84822; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57109; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-octadecanoyl-N-(acetyl)-sphing-4-enine + 2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:57120, ChEBI:CHEBI:46979, ChEBI:CHEBI:74965, ChEBI:CHEBI:76074, ChEBI:CHEBI:76079; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57121; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-N-(acetyl)-sphing-4-enine + 1-octadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:57116, ChEBI:CHEBI:46979, ChEBI:CHEBI:73858, ChEBI:CHEBI:74965, ChEBI:CHEBI:76080; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57117; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-(9Z-octadecenoyl)-2-hexadecanoyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 2-hexadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38767, ChEBI:CHEBI:46979, ChEBI:CHEBI:74667, ChEBI:CHEBI:76054, ChEBI:CHEBI:76078; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38768; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-(9Z-octadecenoyl)-2-hexadecanoyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-hexadecanoyl-N-(acetyl)-sphing-4-enine; Xref=Rhea:RHEA:38763, ChEBI:CHEBI:28610, ChEBI:CHEBI:46979, ChEBI:CHEBI:74667, ChEBI:CHEBI:76077; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38764; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-(9Z)-octadecenoyl-2-octadecanoyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 2-octadecanoyl-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38791, ChEBI:CHEBI:46979, ChEBI:CHEBI:76054, ChEBI:CHEBI:76073, ChEBI:CHEBI:76076; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38792; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1-(9Z)-octadecenoyl-2-octadecanoyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-octadecanoyl-N-(acetyl)-sphing-4-enine; Xref=Rhea:RHEA:38803, ChEBI:CHEBI:28610, ChEBI:CHEBI:46979, ChEBI:CHEBI:76073, ChEBI:CHEBI:76074; Evidence={ECO:0000269|PubMed:16837646};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38804; Evidence={ECO:0000305|PubMed:16837646}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:38703, ChEBI:CHEBI:28610, ChEBI:CHEBI:46979, ChEBI:CHEBI:74669, ChEBI:CHEBI:76054; Evidence={ECO:0000269|PubMed:11790796, ECO:0000269|PubMed:16106046, ECO:0000269|PubMed:16880524, ECO:0000269|PubMed:17626977, ECO:0000269|PubMed:19017977, ECO:0000269|PubMed:20410020, ECO:0000269|PubMed:27993948};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38704; Evidence={ECO:0000305|PubMed:17626977}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + N-(acetyl)-sphing-4-enine = 1-octadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine; Xref=Rhea:RHEA:57140, ChEBI:CHEBI:46979, ChEBI:CHEBI:76074, ChEBI:CHEBI:77342, ChEBI:CHEBI:78260; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57141; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 1-octadecanoyl-sn-glycero-3-phosphoserine; Xref=Rhea:RHEA:57136, ChEBI:CHEBI:46979, ChEBI:CHEBI:76054, ChEBI:CHEBI:78260, ChEBI:CHEBI:84467; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57137; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + N-(acetyl)-sphing-4-enine = 1-octadecanoyl-N-(acetyl)-sphing-4-enine + 2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol); Xref=Rhea:RHEA:57144, ChEBI:CHEBI:46979, ChEBI:CHEBI:72845, ChEBI:CHEBI:76074, ChEBI:CHEBI:141490; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57145; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + N-(acetyl)-sphing-4-enine = 1-(9Z-octadecenoyl)-N-(acetyl)-sphing-4-enine + 1-octadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol); Xref=Rhea:RHEA:57148, ChEBI:CHEBI:46979, ChEBI:CHEBI:72827, ChEBI:CHEBI:72845, ChEBI:CHEBI:76054; Evidence={ECO:0000250|UniProtKB:Q8NCC3};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57149; Evidence={ECO:0000250|UniProtKB:Q8NCC3}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 2-[(5Z,8Z,11Z,14Z)-eicosatetraenoylamino]ethyl (9Z)-octadecenoate; Xref=Rhea:RHEA:38751, ChEBI:CHEBI:2700, ChEBI:CHEBI:74669, ChEBI:CHEBI:76070, ChEBI:CHEBI:76083; Evidence={ECO:0000269|PubMed:17626977};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38752; Evidence={ECO:0000305|PubMed:17626977}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + N-(9Z-octadecenoyl) ethanolamine = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 2-[(9Z)-octadecenoylamino]ethyl (9Z)-octadecenoate; Xref=Rhea:RHEA:38747, ChEBI:CHEBI:71466, ChEBI:CHEBI:74669, ChEBI:CHEBI:76068, ChEBI:CHEBI:76083; Evidence={ECO:0000269|PubMed:17626977};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38748; Evidence={ECO:0000305|PubMed:17626977}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 3-(9Z-octadecenoyl)-sn-glycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1,3-di-(9Z-octadecenoyl)-glycerol; Xref=Rhea:RHEA:38743, ChEBI:CHEBI:74669, ChEBI:CHEBI:75735, ChEBI:CHEBI:75938, ChEBI:CHEBI:76083; Evidence={ECO:0000269|PubMed:17626977};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38744; Evidence={ECO:0000305|PubMed:17626977}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-(9Z-octadecenoyl)-sn-glycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1,3-di-(9Z-octadecenoyl)-glycerol; Xref=Rhea:RHEA:38739, ChEBI:CHEBI:74669, ChEBI:CHEBI:75735, ChEBI:CHEBI:75757, ChEBI:CHEBI:76083; Evidence={ECO:0000269|PubMed:17626977};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38740; Evidence={ECO:0000305|PubMed:17626977}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 2-hexadecanoylglycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-(9Z)-octadecenoyl-2-hexadecanoylglycerol; Xref=Rhea:RHEA:38735, ChEBI:CHEBI:74669, ChEBI:CHEBI:75455, ChEBI:CHEBI:76065, ChEBI:CHEBI:76083; Evidence={ECO:0000269|PubMed:17626977};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38736; Evidence={ECO:0000305|PubMed:17626977}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 3-hexadecanoyl-sn-glycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol; Xref=Rhea:RHEA:38731, ChEBI:CHEBI:64757, ChEBI:CHEBI:74669, ChEBI:CHEBI:75867, ChEBI:CHEBI:76083; Evidence={ECO:0000269|PubMed:17626977};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38732; Evidence={ECO:0000305|PubMed:17626977}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = (9Z)-octadecenoate + (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H(+); Xref=Rhea:RHEA:38699, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:74669, ChEBI:CHEBI:76083; Evidence={ECO:0000269|PubMed:17626977};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38700; Evidence={ECO:0000305|PubMed:17626977}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-O-hexadecyl-2-acetyl-sn-glycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-O-hexadecyl-2-acetyl-3-(9Z)-octadecenoyl-sn-glycerol; Xref=Rhea:RHEA:38707, ChEBI:CHEBI:74669, ChEBI:CHEBI:75936, ChEBI:CHEBI:76055, ChEBI:CHEBI:76083; Evidence={ECO:0000269|PubMed:17626977};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38708; Evidence={ECO:0000305|PubMed:17626977}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-O-hexadecylglycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-O-hexadecyl-3-(9Z)-octadecenoylglycerol; Xref=Rhea:RHEA:38711, ChEBI:CHEBI:74669, ChEBI:CHEBI:76061, ChEBI:CHEBI:76062, ChEBI:CHEBI:76083; Evidence={ECO:0000269|PubMed:17626977};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38712; Evidence={ECO:0000305|PubMed:17626977}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-O-hexadecyl-2-O-methyl-sn-glycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-O-hexadecyl-2-O-methyl-3-(9Z)-octadecenoyl-sn-glycerol; Xref=Rhea:RHEA:38723, ChEBI:CHEBI:74669, ChEBI:CHEBI:76063, ChEBI:CHEBI:76064, ChEBI:CHEBI:76083; Evidence={ECO:0000269|PubMed:17626977};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38724; Evidence={ECO:0000305|PubMed:17626977}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-hexadecanoyl-sn-glycerol = (9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-hexadecanoyl-3-(9Z)-octadecenoyl-sn-glycerol; Xref=Rhea:RHEA:38727, ChEBI:CHEBI:74669, ChEBI:CHEBI:75542, ChEBI:CHEBI:75868, ChEBI:CHEBI:76083; Evidence={ECO:0000269|PubMed:17626977};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38728; Evidence={ECO:0000305|PubMed:17626977};
DNA Binding
EC Number 3.1.1.5; 2.3.1.-; 3.1.1.32; 3.1.1.4
Enzyme Function FUNCTION: Has dual calcium-independent phospholipase and O-acyltransferase activities with a potential role in glycerophospholipid homeostasis and remodeling of acyl groups of lipophilic alcohols present in acidic cellular compartments (PubMed:16837646, PubMed:17626977, PubMed:11790796, PubMed:16106046, PubMed:16880524, PubMed:19017977, PubMed:20410020). Catalyzes hydrolysis of the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 or A2 activity) and transfer it to the hydroxyl group at the first carbon of lipophilic alcohols (O-acyltransferase activity). Among preferred fatty acyl donors are phosphatidylcholines, phosphatidylethanolamines, phosphatidylglycerols and phosphatidylserines (PubMed:20410020). Favors sn-2 over sn-1 deacylation of unsaturated fatty acyl groups of phosphatidylcholines and phosphatidylethanolamines (PubMed:16837646, PubMed:17626977). Among preferred fatty acyl acceptors are natural lipophilic alcohols including short-chain ceramide N-acetyl-sphingosine (C2 ceramide), alkylacylglycerols, monoacylglycerols, and acylethanolamides such as anandamide and oleoylethanolamide (PubMed:16837646, PubMed:17626977). Selectively hydrolyzes the sn-1 fatty acyl group of truncated oxidized phospholipids and may play a role in detoxification of reactive oxidized phospholipids during oxidative stress (PubMed:27993948). Required for normal phospholipid degradation in alveolar macrophages with potential implications in pulmonary surfactant clearance (PubMed:16880524, PubMed:19017977). At neutral pH, hydrolyzes the sn-1 fatty acyl group of the lysophosphatidylcholines (By similarity). {ECO:0000250|UniProtKB:Q8NCC3, ECO:0000269|PubMed:11790796, ECO:0000269|PubMed:16106046, ECO:0000269|PubMed:16837646, ECO:0000269|PubMed:16880524, ECO:0000269|PubMed:17626977, ECO:0000269|PubMed:19017977, ECO:0000269|PubMed:20410020, ECO:0000269|PubMed:27993948}.
Temperature Dependency
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4.5. {ECO:0000305|PubMed:27993948};
Pathway
nucleotide Binding
Features Active site (3); Binding site (2); Chain (1); Disulfide bond (1); Glycosylation (4); Metal binding (3); Mutagenesis (5); Sequence conflict (1); Signal peptide (1)
Keywords Acyltransferase;Disulfide bond;Fatty acid metabolism;Glycoprotein;Hydrolase;Lipid metabolism;Lysosome;Membrane;Metal-binding;Reference proteome;Secreted;Signal;Transferase;Zinc
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19017977, ECO:0000269|PubMed:20410020}. Lysosome {ECO:0000269|PubMed:19017977}. Membrane {ECO:0000250|UniProtKB:Q8NCC3}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q8NCC3}.
Modified Residue
Post Translational Modification PTM: N-glycosylated (PubMed:11790796). N-glycosylation is important for maturation of the enzyme and normal subcellular location (By similarity). {ECO:0000250|UniProtKB:Q8NCC3, ECO:0000269|PubMed:11790796}.
Signal Peptide SIGNAL 1..33; /evidence=ECO:0000250|UniProtKB:Q8NCC3
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 10725249; 12904583; 14681479; 15294901; 15537664; 15781238; 16615898; 18614015; 18799693; 20562862; 23493550; 24006456; 25871848; 29514215; 32777483;
Motif
Gene Encoded By
Mass 47,307
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=171 uM for 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine (O-acyltransferase activity) {ECO:0000269|PubMed:20410020}; Vmax=5.55 umol/min/mg enzyme toward 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine (O-acyltransferase activity) {ECO:0000269|PubMed:20410020};
Metal Binding METAL 198; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q8NCC3; METAL 355; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q8NCC3; METAL 392; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q8NCC3
Rhea ID RHEA:18689; RHEA:18690; RHEA:38783; RHEA:38784; RHEA:62480; RHEA:62481; RHEA:62464; RHEA:62465; RHEA:62484; RHEA:62485; RHEA:62488; RHEA:62489; RHEA:15801; RHEA:15802; RHEA:38779; RHEA:38780; RHEA:15177; RHEA:15178; RHEA:40435; RHEA:40436; RHEA:38827; RHEA:38828; RHEA:38823; RHEA:38824; RHEA:38831; RHEA:38832; RHEA:38835; RHEA:38836; RHEA:38843; RHEA:38844; RHEA:38839; RHEA:38840; RHEA:38759; RHEA:38760; RHEA:38755; RHEA:38756; RHEA:38811; RHEA:38812; RHEA:38807; RHEA:38808; RHEA:38775; RHEA:38776; RHEA:38771; RHEA:38772; RHEA:38815; RHEA:38816; RHEA:38819; RHEA:38820; RHEA:62472; RHEA:62473; RHEA:38799; RHEA:38800; RHEA:38795; RHEA:38796; RHEA:57108; RHEA:57109; RHEA:57120; RHEA:57121; RHEA:57116; RHEA:57117; RHEA:38767; RHEA:38768; RHEA:38763; RHEA:38764; RHEA:38791; RHEA:38792; RHEA:38803; RHEA:38804; RHEA:38703; RHEA:38704; RHEA:57140; RHEA:57141; RHEA:57136; RHEA:57137; RHEA:57144; RHEA:57145; RHEA:57148; RHEA:57149; RHEA:38751; RHEA:38752; RHEA:38747; RHEA:38748; RHEA:38743; RHEA:38744; RHEA:38739; RHEA:38740; RHEA:38735; RHEA:38736; RHEA:38731; RHEA:38732; RHEA:38699; RHEA:38700; RHEA:38707; RHEA:38708; RHEA:38711; RHEA:38712; RHEA:38723; RHEA:38724; RHEA:38727; RHEA:38728
Cross Reference Brenda