Detail Information for IndEnz0005000820
IED ID IndEnz0005000820
Enzyme Type ID lipase000820
Protein Name Triacylglycerol lipase ptl1
EC 3.1.1.3
Gene Name ptl1 SPCC1450.16c
Organism Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota Taphrinomycotina Schizosaccharomycetes Schizosaccharomycetales Schizosaccharomycetaceae Schizosaccharomyces Schizosaccharomyces pombe (Fission yeast) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Enzyme Sequence MTALFNFWRIMYANISSIWLLVVSFFEWLFSATSISQQRGSGPRKGKVVMNKDCRSWEDWKVLATTIDKASGRWKWRFTPASDKYDYLLIDRCTVSLKRYRQRKSVYPMLMFLRSSLLRNFGNIGNSSLYTENYSGTKILIEEYVREVNNCLEFLYHTKRLSYDVKCDFFSAARISFGTTCLYFNGGTAFGLYHFGVAKTLWKRNLLPQILAGCASGALIASLLSVYRDEELNGLFDTFPSELWKICQQTSDYSLSKVVEYGNMLDISMIASFVRQRLGTITFQEAFERTGRIVNIVAPPSAVSGSPQVLNYFTAPNVLIWSAVCSSNSWAAIYRSSPLLAKLPDGSTEVCTPKNFIWPYAGLPNTGRSNPYARISEIFNVNHFVITQSRPSLFPTFYDELHHHRVSGYSLKMIRLVGLEMAYRFRQLDILGLLPPRLRRFFVDDYVPSAYITLTPTFSFSDIKHAFTKPSLSDIQYWILVGERATWQAIPLLQVRCKTEISLRHLSKNLTNSYVEPLSVNNLASPFVTNLEENQEKMLKIFKVK
Enzyme Length 545
Uniprot Accession Number Q9Y7P3
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; Evidence={ECO:0000250|UniProtKB:P40308};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:12045; Evidence={ECO:0000250|UniProtKB:P40308};
DNA Binding
EC Number 3.1.1.3
Enzyme Function FUNCTION: Lipid particle-localized triacylglycerol (TAG) lipase. The lipid droplet/particle is a lipid storage compartment which serves as a depot of energy and building blocks for membrane lipid biosynthesis. Involved in the mobilization of the non-polar storage lipids triacylglycerols (TAGs) from lipid particles by hydrolysis of TAGs, releasing and supplying specific fatty acids to the appropriate metabolic pathways. {ECO:0000269|PubMed:22592553}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Domain (1); Motif (1)
Keywords Hydrolase;Lipid degradation;Lipid droplet;Lipid metabolism;Reference proteome
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Lipid droplet {ECO:0000250|UniProtKB:P40308}. Note=Partially retained in the endoplasmic reticulum in cells lacking triacylglycerols. {ECO:0000250|UniProtKB:P40308}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 20473289; 23697806; 25452419; 34250083;
Motif MOTIF 213..217; /note=GXSXG
Gene Encoded By
Mass 62,546
Kinetics
Metal Binding
Rhea ID RHEA:12044; RHEA:12045
Cross Reference Brenda