| IED ID | IndEnz0005000821 |
| Enzyme Type ID | lipase000821 |
| Protein Name |
Putative monoglyceride lipase MGL EC 3.1.1.23 Fatty acid ethyl ester hydrolase FAEE hydrolase Monoacylglycerol hydrolase MAG hydrolase MGH Monoacylglycerol lipase MAG lipase MAGL |
| Gene Name | mgl1 SPCC5E4.05c |
| Organism | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota Taphrinomycotina Schizosaccharomycetes Schizosaccharomycetales Schizosaccharomycetaceae Schizosaccharomyces Schizosaccharomyces pombe (Fission yeast) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Enzyme Sequence | MADLYTKDWTDVKDKPVARVVFIHGFGEHVNAYPEFFEALNERNIEVYTFDQRGFGHSRKGGPKKQGCTGGWSLVFPDLDYQILRASDTELPLFLWGHSMGGGLALRYGISGTHRHKLAGVIAQAPMLRCHPDTEPNFLLRKALTLVSKVHPNFLFDSDVQSQHITRDEAVNQRLQDDPLVSSVGSLQVFSDMLNRGTKTIELAPQFFLPLLITHGTDDNVTCSDSSKEFYENAGTKDKTYQSYPGFYHSLHIEKKPEVYEYLDKVAAWIYEHSKPSETVKSEQETAVEHPKPTATTSAPSASPTGVPVEEESHKATSDAVPPAEAKPEPVPASAAERAPTSESTTVPETIVASTTKVISEPAPRVTTAATADIVTNK |
| Enzyme Length | 378 |
| Uniprot Accession Number | O94305 |
| Absorption | |
| Active Site | ACT_SITE 99; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P28321; ACT_SITE 219; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P28321; ACT_SITE 249; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P28321 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolyzes glycerol monoesters of long-chain fatty acids.; EC=3.1.1.23; Evidence={ECO:0000250|UniProtKB:P28321}; |
| DNA Binding | |
| EC Number | 3.1.1.23 |
| Enzyme Function | FUNCTION: Converts monoacylglycerides (MAG) to free fatty acids and glycerol. Has a strong preference for monounsaturated monoglycerides. Required for efficient degradation of MAG, short-lived intermediates of glycerolipid metabolism which may also function as lipid signaling molecules. Controls inactivation of the signaling lipid N-palmitoylethanolamine (PEA). Involved in fatty acid ethyl ester (FAEE) catabolism. FAEEs are non-oxidative metabolites of ethanol that are transiently incorporated into lipid droplets (LDs). Their mobilization by LD-resident FAEE hydrolases facilitates a controlled metabolism of these potentially toxic lipid metabolites. {ECO:0000250|UniProtKB:P28321}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycerolipid metabolism; triacylglycerol degradation. {ECO:0000250|UniProtKB:P28321}. |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Compositional bias (1); Modified residue (1); Motif (1); Region (1) |
| Keywords | Cytoplasm;Endoplasmic reticulum;Hydrolase;Lipid droplet;Membrane;Mitochondrion;Mitochondrion outer membrane;Phosphoprotein;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Lipid droplet {ECO:0000250|UniProtKB:P28321}. Cytoplasm {ECO:0000269|PubMed:16823372}. Endoplasmic reticulum {ECO:0000250|UniProtKB:P28321}. Mitochondrion outer membrane {ECO:0000250|UniProtKB:P28321}. Note=Although the protein is identified in the cytoplasm, several membrane systems and lipid droplets, MGL activity is only measured in membrane fractions and lipid droplets. {ECO:0000250|UniProtKB:P28321}. |
| Modified Residue | MOD_RES 301; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18257517 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 19547744; 19750511; 20473289; 21712547; 23697806; 25720772; 26537787; 26644575; 27298342; 28410370; 29996109; 30647105; 30726745; |
| Motif | MOTIF 97..101; /note=GXSXG; /evidence=ECO:0000250|UniProtKB:P28321 |
| Gene Encoded By | |
| Mass | 41,624 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |