| IED ID | IndEnz0005000837 |
| Enzyme Type ID | lipase000837 |
| Protein Name |
Triacylglycerol lipase SDP1L EC 3.1.1.3 Protein SDP1-LIKE |
| Gene Name | SDP1L At3g57140 F24I3.220 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MDISNEAGVDAFSIIGPTTIIGRTIAVRILFCNSVSIFRHKVFRILKFFLRGGRVLLSPFVSLLHPRNPQGILVMVTTMAFLLNRYTSLKAKAEMAYRRKFWRNMMRAALTYEEWSHAAKMLDKETPKVNETDLFDVELVSNKLDELKHRRHEGSLRDIIFCMRADLVRNLGNMCNPELHKGRLHVPRLIKEYIDEVSTQLRMVCDMDTEELSLEEKLSFMHETRHAYGRTALLLSGGASLGAFHLGVVKTLVEHKLLPRIIAGSSVGSVMCAVVGTRSWPELQSFFEGSWHALQFFDQMGGIFTTVKRVMTQGAVHEIRHLQWKLRNLTNNLTFQEAYDITGRILGITVCSLRKHEPPRCLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKDRTGEIVPYHPPFNLDPEEGSASVRRWRDGSLEMDLPMIQLKELFNVNHFIVSQANPHIAPFLRMKEFVRACGGRFAAKLAQLAEMEVKHRCNQVLELGLPLREVASLFAQEWEGDVTIVMPATFSQYLKIIQNPSNVEIQKAANQGRRCTWEKLAVIKANFGIELALDECVTVLNHMRRLKRSAERAAAFSAISSSPPSKHLLAGTNRFNASKRIPSWNCIARQNSSGSVDDDVLAEASRLYQHIVVGSGRNSNRTSNLSHTYDAGSECDSPEAEDWTRSGGPLMRTNSAQMFTDYVQNLDAVDPEQIRASENDSIVAASSSSHSITVTEGDYLQTGRTHNGFVLNLVRGENLRMNSEPEDSQNESEIPETPESVQLDSPEKDIIDGESSASEDGDAQANLIHDHE |
| Enzyme Length | 801 |
| Uniprot Accession Number | Q9M1I6 |
| Absorption | |
| Active Site | ACT_SITE 266; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 423; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; Evidence={ECO:0000269|PubMed:19136267}; |
| DNA Binding | |
| EC Number | 3.1.1.3 |
| Enzyme Function | FUNCTION: May be involved in the release of fatty acids from the oil body in germinating seedlings. Can hydrolyze triacylglycerols in vitro. {ECO:0000269|PubMed:19136267}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (1); Glycosylation (9); Motif (1); Region (2); Transmembrane (2) |
| Keywords | Glycoprotein;Hydrolase;Lipid degradation;Lipid droplet;Lipid metabolism;Membrane;Reference proteome;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Lipid droplet {ECO:0000250|UniProtKB:Q9LZA6}. Membrane {ECO:0000250|UniProtKB:Q9LZA6}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q9LZA6}. Note=Associates with the oil body membrane. {ECO:0000250|UniProtKB:Q9LZA6}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 16473965; 18775970; 21825108; 23505340; |
| Motif | MOTIF 264..268; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Gene Encoded By | |
| Mass | 89,934 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:12044 |
| Cross Reference Brenda |