| IED ID | IndEnz0005000840 |
| Enzyme Type ID | lipase000840 |
| Protein Name |
Thyroglobulin Tg |
| Gene Name | TG |
| Organism | Bos taurus (Bovine) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Ruminantia Pecora Bovidae Bovinae Bos (oxen cattle) Bos taurus (Bovine) |
| Enzyme Sequence | MALALWVFGLLDLICLASANIFEYQVDAQPLRPCELQRERAFLKREDYVPQCAEDGSFQTVQCGKDGASCWCVDADGREVPGSRQPGRPAACLSFCQLQKQQILLSSYINSTATSYLPQCQDSGDYSPVQCDLRRRQCWCVDAEGMEVYGTRQQGRPARCPRSCEIRNRRLLHGVGDRSPPQCSPDGAFRPVQCKLVNTTDMMIFDLVHSYSRFPDAFVTFSSFRSRFPEVSGYCYCADSQGRELAETGLELLLDEIYDTIFAGLDLASTFAETTLYRILQRRFLAVQLVISGRFRCPTKCEVERFAATSFRHPYVPSCHPDGEYQAAQCQQGGPCWCVDSRGQEIPGTRQRGEPPSCAEDQSCPSERRRAFSRLRFGPSGYFSRRSLLLAPEEGPVSQRFARFTASCPPSIKELFLDSGIFQPMLQGRDTRFVAPESLKEAIRGLFPSRELARLALQFTTNAKRLQQNLFGGRFLVKVGQFNLSGALGTRGTFNFSHFFQQLGLPGFQDGRALADLAKPLSVGLNSNPASEAPKASKIDVALRKPVVGSFGFEVNLQENQNALQFLSSFLELPEFLLFLQHAISVPEDIARDLGDVMEMVFSSQGCGQAPGSLFVPACTAEGSYEEVQCFAGDCWCVDAQGRELAGSRVRGGRPRCPTECEKQRARMQSLLGSQPAGSSLFVPACTSKGNFLPVQCFNSECYCVDTEGQPIPGTRSALGEPKKCPSPCQLQAERAFLGTVRTLVSNPSTLPALSSIYIPQCSASGQWSPVQCDGPPEQAFEWYERWEAQNSAGQALTPAELLMKIMSYREAASRNFRLFIQNLYEAGQQGIFPGLARYSSFQDVPVSVLEGNQTQPGGNVFLEPYLFWQILNGQLDRYPGPYSDFSAPLAHFDLRSCWCVDEAGQKLEGTRNEPNKVPACPGSCEEVKLRVLQFIREAEEIVTYSNSSRFPLGESFLAAKGIRLTDEELAFPPLSPSRETFLEKFLSGSDYAIRLAAQSTFDFYQRRLVTLAESPRAPSPVWSSAYLPQCDAFGGWEPVQCHAATGHCWCVDGKGEYVPTSLTARSRQIPQCPTSCERLRASGLLSSWKQAGVQAEPSPKDLFIPTCLETGEFARLQASEAGTWCVDPASGEGVPPGTNSSAQCPSLCEVLQSGVPSRRTSPGYSPACRAEDGGFSPVQCDPAQGSCWCVLGSGEEVPGTRVAGSQPACESPQCPLPFSVADVAGGAILCERASGLGAAAGQRCQLRCSQGYRSAFPPEPLLCSVQRRRWESRPPQPRACQRPQFWQTLQTQAQFQLLLPLGKVCSADYSGLLLAFQVFLLDELTARGFCQIQVKTAGTPVSIPVCDDSSVKVECLSRERLGVNITWKLQLVDAPPASLPDLQDVEEALAGKYLAGRFADLIQSGTFQLHLDSKTFSADTSIRFLQGDRFGTSPRTQFGCLEGFGRVVAASDASQDALGCVKCPEGSYFQDEQCIPCPAGFYQEQAGSLACVPCPEGRTTVYAGAFSQTHCVTDCQKNEVGLQCDQDSQYRASQRDRTSGKAFCVDGEGRRLPWTEAEAPLVDAQCLVMRKFEKLPESKVIFSADVAVMVRSEVPGSESSLMQCLADCALDEACGFLTVSTAGSEVSCDFYAWASDSIACTTSGRSEDALGTSQATSFGSLQCQVKVRSREGDPLAVYLKKGQEFTITGQKRFEQTGFQSALSGMYSPVTFSASGASLAEVHLFCLLACDHDSCCDGFILVQVQGGPLLCGLLSSPDVLLCHVRDWRDPAEAQANASCPGVTYDQDSRQVTLRLGGQEIRGLTPLEGTQDTLTSFQQVYLWKDSDMGSRSESMGCRRDTEPRPASPSETDLTTGLFSPVDLIQVIVDGNVSLPSQQHWLFKHLFSLQQANLWCLSRCAGEPSFCQLAEVTDSEPLYFTCTLYPEAQVCDDILESSPKGCRLILPRRPSALYRKKVVLQDRVKNFYNRLPFQKLTGISIRNKVPMSDKSISSGFFECERLCDMDPCCTGFGFLNVSQLKGGEVTCLTLNSLGLQTCSEEYGGVWRILDCGSPDTEVRTYPFGWYQKPVSPSDAPSFCPSVALPALTENVALDSWQSLALSSVIVDPSIRNFDVAHISTAAVGNFSAARDRCLWECSRHQDCLVTTLQTQPGAVRCMFYADTQSCTHSLQAQNCRLLLHEEATYIYRKPNIPLPGFGTSSPSVPIATHGQLLGRSQAIQVGTSWKPVDQFLGVPYAAPPLGEKRFRAPEHLNWTGSWEATKPRARCWQPGIRTPTPPGVSEDCLYLNVFVPQNMAPNASVLVFFHNAAEGKGSGDRPAVDGSFLAAVGNLIVVTASYRTGIFGFLSSGSSELSGNWGLLDQVVALTWVQTHIQAFGGDPRRVTLAADRGGADIASIHLVTTRAANSRLFRRAVLMGGSALSPAAVIRPERARQQAAALAKEVGCPSSSVQEMVSCLRQEPARILNDAQTKLLAVSGPFHYWGPVVDGQYLRETPARVLQRAPRVKVDLLIGSSQDDGLINRAKAVKQFEESQGRTSSKTAFYQALQNSLGGEAADAGVQAAATWYYSLEHDSDDYASFSRALEQATRDYFIICPVIDMASHWARTVRGNVFMYHAPESYSHSSLELLTDVLYAFGLPFYPAYEGQFTLEEKSLSLKIMQYFSNFIRSGNPNYPHEFSRRAPEFAAPWPDFVPRDGAESYKELSVLLPNRQGLKKADCSFWSKYIQSLKASADETKDGPSADSEEEDQPAGSGLTEDLLGLPELASKTYSK |
| Enzyme Length | 2769 |
| Uniprot Accession Number | P01267 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3) (By similarity). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling (By similarity). Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream (By similarity). One dimer produces 7 thyroid hormone molecules (By similarity). {ECO:0000250|UniProtKB:F1RRV3, ECO:0000250|UniProtKB:O08710, ECO:0000250|UniProtKB:P01266}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (1); Disulfide bond (58); Domain (11); Glycosylation (22); Modified residue (36); Region (3); Repeat (8); Sequence conflict (1); Signal peptide (1); Site (1) |
| Keywords | Disulfide bond;Glycoprotein;Hormone;Iodination;Reference proteome;Repeat;Secreted;Signal;Sulfation;Thyroid hormone;Thyroid hormones biosynthesis |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:O08710}. Note=Secreted into the thyroid follicle lumen. Localizes to colloid globules, a structure formed in the thyroid follicle lumen consisting of cross-linked TG arranged in concentric layers. {ECO:0000250|UniProtKB:O08710}. |
| Modified Residue | MOD_RES 24; /note=Iodotyrosine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 24; /note=Sulfotyrosine; alternate; /evidence=ECO:0000250|UniProtKB:F1RRV3; MOD_RES 24; /note=Thyroxine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 24; /note=Triiodothyronine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 108; /note=Iodotyrosine; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 149; /note=Diiodotyrosine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 149; /note=Iodotyrosine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 234; /note=Iodotyrosine; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 258; /note=Iodotyrosine; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 703; /note=Diiodotyrosine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 703; /note=Iodotyrosine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 703; /note=Thyroxine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 703; /note=Triiodothyronine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 784; /note=Iodotyrosine; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 866; /note=Diiodotyrosine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 866; /note=Iodotyrosine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 883; /note=Diiodotyrosine; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 992; /note=Diiodotyrosine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 992; /note=Iodotyrosine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 1310; /note=Iodotyrosine; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 1310; /note=Thyroxine; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 1469; /note=Diiodotyrosine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 1469; /note=Iodotyrosine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 2185; /note=Iodotyrosine; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 2541; /note=Thyroxine; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 2574; /note=Diiodotyrosine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 2574; /note=Iodotyrosine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 2574; /note=Thyroxine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 2574; /note=Triiodothyronine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 2588; /note=Iodotyrosine; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 2618; /note=Iodotyrosine; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 2698; /note=Diiodotyrosine; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 2767; /note=Diiodotyrosine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 2767; /note=Iodotyrosine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 2767; /note=Thyroxine; alternate; /evidence=ECO:0000250|UniProtKB:P01266; MOD_RES 2767; /note=Triiodothyronine; alternate; /evidence=ECO:0000250|UniProtKB:P01266 |
| Post Translational Modification | PTM: Iodinated on tyrosine residues by TPO (By similarity). There are 4 pairs of iodinated tyrosines used for coupling: acceptor Tyr-24 is coupled to donor Tyr-149 or Tyr-234, acceptor Tyr-2574 is coupled to donor Tyr-2541, acceptor Tyr-2767 in monomer 1 is coupled to donor Tyr-2767 in monomer 2 and acceptor Tyr-1310 in monomer 1 is coupled to donor Tyr-108 in monomer 2 (By similarity). {ECO:0000250|UniProtKB:F1RRV3, ECO:0000250|UniProtKB:P01266}.; PTM: Sulfated tyrosines are desulfated during iodination. {ECO:0000250|UniProtKB:P01266}.; PTM: Undergoes sequential proteolysis by cathepsins to release thyroxine (T4) and triiodothyronine (T3) hormones. In the thyroid follicle lumen, cross-linked TG (storage form) is solubilized by limited proteolysis mediated by cathepsins CTSB and/or CTSL. Partially cleaved TG is further processed by CTSK/cathepsin K and/or CTSL resulting in the release of T4. Following endocytosis, further processing occurs leading to the release of T3 and more T4 hormones. {ECO:0000250|UniProtKB:O08710}. |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000250|UniProtKB:F1RRV3 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 303,222 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |