Detail Information for IndEnz0005000847
IED ID IndEnz0005000847
Enzyme Type ID lipase000847
Protein Name Triacylglycerol lipase OBL1
EC 3.1.1.3
Oil body lipase 1
RcOBL1
Triacylglycerol acidic lipase OBL1
Gene Name OBL1 RCOM_1098580
Organism Ricinus communis (Castor bean)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Malpighiales Euphorbiaceae Acalyphoideae Acalypheae Ricinus Ricinus communis (Castor bean)
Enzyme Sequence MDDAGKITSTSHLIVSPDEGTFLDLFKHIVLSDLGSGAKFFRASDQRVPATAAYYSRWPVSVFICKILQLFQMPAAMLGHLTDFLLNFYYQNHGFLGILRNIFLIRLKIPKRGEADFISTIGYLDSRMDLHGTPMVSHQADEVISNADNPSLKEGHNSKIKGALGNRSLMDLCIMASKLAYENTKVVERVVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDANLIVISFRGTGPFSIPNWCTDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSINTKSPGSITELRKESEMAPDHLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGALAILFTCILEIQQETEVLDRLLNVYTFGQPRIGNYNLGYFMQNRLNFPERRYFRVVYCNDMVPRVPFDDVFFTFEHFGTCIYYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSFILNHVYGAEYKETWESRMFRILGLFLPGVAAHSPVNYVNSVRLGRELAIPLMSLKMMAQGY
Enzyme Length 526
Uniprot Accession Number Q5VKJ7
Absorption
Active Site ACT_SITE 340; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:F4JFU8; ACT_SITE 404; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q948R1; ACT_SITE 497; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q948R1
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; Evidence={ECO:0000269|PubMed:15322116};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:12045; Evidence={ECO:0000269|PubMed:15322116};
DNA Binding
EC Number 3.1.1.3
Enzyme Function FUNCTION: Acid lipase that can hydrolyze a range of triacylglycerols but is not active on phospholipids (PubMed:15322116). In vitro, hydrolyzes triolein, trilinolein, triricinolein, tripalmitin, trilaurin and tricaprin (PubMed:15322116). May play a role in the regulation of lipolysis in germinating seeds (PubMed:15322116). {ECO:0000269|PubMed:15322116}.
Temperature Dependency
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4-4.5. {ECO:0000269|PubMed:15322116};
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Motif (1); Transmembrane (1)
Keywords Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane protein {ECO:0000255}. Note=Associates with the oil body membrane in seed endosperm. {ECO:0000269|PubMed:15322116}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif MOTIF 338..342; /note=GXSXG; /evidence=ECO:0000250|UniProtKB:F4JFU8
Gene Encoded By
Mass 59,627
Kinetics
Metal Binding
Rhea ID RHEA:12044; RHEA:12045
Cross Reference Brenda 3.1.1.3;