Detail Information for IndEnz0005000854
IED ID IndEnz0005000854
Enzyme Type ID lipase000854
Protein Name Medium-chain fatty acid ethyl ester synthase/esterase 2
Alcohol O-acetyltransferase
EC 2.3.1.84
EC 3.1.1.-
Ethanol hexanoyl transferase 1
Gene Name EHT1 YBR177C YBR1239
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Enzyme Sequence MSEVSKWPAINPFHWGYNGTVSHIVGENGSIKLHLKDNKEQVDFDEFANKYVPTLKNGAQFKLSPYLFTGILQTLYLGAADFSKKFPVFYGREIVKFSDGGVCTADWLIDSWKKDYEFDQSTTSFDKKKFDKDEKATHPEGWPRLQPRTRYLKDNELEELREVDLPLVVILHGLAGGSHEPIIRSLAENLSRSGRFQVVVLNTRGCARSKITTRNLFTAYHTMDIREFLQREKQRHPDRKLYAVGCSFGATMLANYLGEEGDKSPLSAAATLCNPWDLLLSAIRMSQDWWSRTLFSKNIAQFLTRTVQVNMGELGVPNGSLPDHPPTVKNPSFYMFTPENLIKAKSFKSTREFDEVYTAPALGFPNAMEYYKAASSINRVDTIRVPTLVINSRDDPVVGPDQPYSIVEKNPRILYCRTDLGGHLAYLDKDNNSWATKAIAEFFTKFDELVV
Enzyme Length 451
Uniprot Accession Number P38295
Absorption
Active Site ACT_SITE 247; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 395; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 423; /note=Charge relay system; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=acetyl-CoA + an aliphatic alcohol = an acetyl ester + CoA; Xref=Rhea:RHEA:17229, ChEBI:CHEBI:2571, ChEBI:CHEBI:47622, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.84;
DNA Binding
EC Number 2.3.1.84; 3.1.1.-
Enzyme Function FUNCTION: Displays enzymatic activity both for medium-chain fatty acid (MCFA) ethyl ester synthesis and hydrolysis (esterase activity). MCFA are toxic for yeast and this enzyme could thus be involved in their detoxification by esterification. {ECO:0000269|PubMed:16361250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Cross-link (1); Domain (1)
Keywords Acyltransferase;Hydrolase;Isopeptide bond;Reference proteome;Serine esterase;Transferase;Ubl conjugation
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 10515935; 10998572; 11015726; 11805826; 14645503; 16002012; 16407407; 16429126; 16606443; 17993562; 18467557; 19696439; 19841731; 21255318; 22940803; 23217712; 23275493; 23464572; 24370880; 24384752; 24868093; 25028499; 25308280; 27693354;
Motif
Gene Encoded By
Mass 51,255
Kinetics
Metal Binding
Rhea ID RHEA:17229
Cross Reference Brenda