| IED ID | IndEnz0005000860 |
| Enzyme Type ID | lipase000860 |
| Protein Name |
Lipid droplet hydrolase 1 EC 3.1.1.3 Lipid esterase Triacylglycerol lipase |
| Gene Name | LDH1 YBR204C YBR1444 |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MNMAERAEATKSWSCEPLSGKTLEEIVQNAENAADLVAYIRKPEVDLDFRLKFIAEHEEFFNVQLSDRNSRIRTCHNLSDKGIRGDTVFVFVPGLAGNLEQFEPLLELVDSDQKAFLTLDLPGFGHSSEWSDYPMLKVVELIFVLVCDVLRKWSTAVPNNDNVNPFNGHKIVLVGHSMGCFLACHLYEQHMADTKAVQTLVLLTPPKAHIEQLSKDKHIIQWALYGVFKLPWLFDVYRNKFDQVKGLQSSGIKQYFYQQGDDVKLKYRKFWQFKNNISNKSRTIIGYLLGWETVDWVKFNGVLTQTDMKQKIIIFGAEKDPIAPIENLEFYKQTINKECLRKVIILPDCSHNLCFDRPELVCENFQREVIDNSKL |
| Enzyme Length | 375 |
| Uniprot Accession Number | P38139 |
| Absorption | |
| Active Site | ACT_SITE 177; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:O75608 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; Evidence={ECO:0000269|PubMed:21478434}; |
| DNA Binding | |
| EC Number | 3.1.1.3 |
| Enzyme Function | FUNCTION: Serine hydrolase required for the maintenance of steady state level of non-polar and polar lipids of lipid droplets and thus plays a role in maintaining the lipids homeostasis (PubMed:21478434). Exhibits both esterase and triacylglycerol lipase activity (PubMed:21478434). {ECO:0000269|PubMed:21478434}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Domain (1); Motif (1); Mutagenesis (1); Sequence conflict (2) |
| Keywords | Hydrolase;Lipid degradation;Lipid droplet;Lipid metabolism;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Lipid droplet {ECO:0000269|PubMed:21478430}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11283351; 11805826; 12140549; 16429126; 19536198; 23275493; 24520995; 24678285; 24868093; 26162625; |
| Motif | MOTIF 373..375; /note=Microbody targeting signal; /evidence=ECO:0000255 |
| Gene Encoded By | |
| Mass | 43,287 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.77 uM for p-nitrophenyl butyrate (PNB) {ECO:0000269|PubMed:21478434}; KM=3.3 mM for 1,2-dioleoyl-3-(pyren-1-yl)-decanoyl-rac-glycerol (DPG) {ECO:0000269|PubMed:21478434}; Vmax=0.041 umol/min/mg enzyme for esterase activity using PNB as a substrate {ECO:0000269|PubMed:21478434}; Vmax=1 umol/min/mg enzyme for triacylglycerol lipase activity using DPG as a substrate {ECO:0000269|PubMed:21478434}; |
| Metal Binding | |
| Rhea ID | RHEA:12044 |
| Cross Reference Brenda |