IED ID | IndEnz0005000860 |
Enzyme Type ID | lipase000860 |
Protein Name |
Lipid droplet hydrolase 1 EC 3.1.1.3 Lipid esterase Triacylglycerol lipase |
Gene Name | LDH1 YBR204C YBR1444 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Enzyme Sequence | MNMAERAEATKSWSCEPLSGKTLEEIVQNAENAADLVAYIRKPEVDLDFRLKFIAEHEEFFNVQLSDRNSRIRTCHNLSDKGIRGDTVFVFVPGLAGNLEQFEPLLELVDSDQKAFLTLDLPGFGHSSEWSDYPMLKVVELIFVLVCDVLRKWSTAVPNNDNVNPFNGHKIVLVGHSMGCFLACHLYEQHMADTKAVQTLVLLTPPKAHIEQLSKDKHIIQWALYGVFKLPWLFDVYRNKFDQVKGLQSSGIKQYFYQQGDDVKLKYRKFWQFKNNISNKSRTIIGYLLGWETVDWVKFNGVLTQTDMKQKIIIFGAEKDPIAPIENLEFYKQTINKECLRKVIILPDCSHNLCFDRPELVCENFQREVIDNSKL |
Enzyme Length | 375 |
Uniprot Accession Number | P38139 |
Absorption | |
Active Site | ACT_SITE 177; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:O75608 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; Evidence={ECO:0000269|PubMed:21478434}; |
DNA Binding | |
EC Number | 3.1.1.3 |
Enzyme Function | FUNCTION: Serine hydrolase required for the maintenance of steady state level of non-polar and polar lipids of lipid droplets and thus plays a role in maintaining the lipids homeostasis (PubMed:21478434). Exhibits both esterase and triacylglycerol lipase activity (PubMed:21478434). {ECO:0000269|PubMed:21478434}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (1); Chain (1); Domain (1); Motif (1); Mutagenesis (1); Sequence conflict (2) |
Keywords | Hydrolase;Lipid degradation;Lipid droplet;Lipid metabolism;Reference proteome |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Lipid droplet {ECO:0000269|PubMed:21478430}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | 11283351; 11805826; 12140549; 16429126; 19536198; 23275493; 24520995; 24678285; 24868093; 26162625; |
Motif | MOTIF 373..375; /note=Microbody targeting signal; /evidence=ECO:0000255 |
Gene Encoded By | |
Mass | 43,287 |
Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.77 uM for p-nitrophenyl butyrate (PNB) {ECO:0000269|PubMed:21478434}; KM=3.3 mM for 1,2-dioleoyl-3-(pyren-1-yl)-decanoyl-rac-glycerol (DPG) {ECO:0000269|PubMed:21478434}; Vmax=0.041 umol/min/mg enzyme for esterase activity using PNB as a substrate {ECO:0000269|PubMed:21478434}; Vmax=1 umol/min/mg enzyme for triacylglycerol lipase activity using DPG as a substrate {ECO:0000269|PubMed:21478434}; |
Metal Binding | |
Rhea ID | RHEA:12044 |
Cross Reference Brenda |