| IED ID | IndEnz0005000864 |
| Enzyme Type ID | lipase000864 |
| Protein Name |
tRNA guanine-N 1 - -methyltransferase EC 2.1.1.228 M1G-methyltransferase tRNA GM37 methyltransferase |
| Gene Name | trmD ACIAD3311 |
| Organism | Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Moraxellales Moraxellaceae Acinetobacter Acinetobacter baylyi Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) |
| Enzyme Sequence | MFFAVITLFPEMFEAITAYGISGRATKRQIATVSCINPRDFAEGNYKRVDERPFGGGPGMVMMAEPLAKAIIHAKQLAEQAGCVHAPVVYMSPQGKTLNEHAVQQFVDYDGLIVLCGRYEGVDERLIQHYVDQEWSIGDYVLSGGELPAMVLLDSIIRRLPDAMSDEQSHIQDSFVDGLLDCPQYTKPDHFEGLDVPEVLKSGHHANIEKWRFLQRYQRTLDRRPELVEKVTLTKQQRKWLTFLDDSKN |
| Enzyme Length | 249 |
| Uniprot Accession Number | Q43963 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 117; /note=S-adenosyl-L-methionine; via amide nitrogen; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:36899, Rhea:RHEA-COMP:10145, Rhea:RHEA-COMP:10147, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:73542, ChEBI:CHEBI:74269; EC=2.1.1.228; |
| DNA Binding | |
| EC Number | 2.1.1.228 |
| Enzyme Function | FUNCTION: Specifically methylates guanosine-37 in various tRNAs. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Binding site (1); Chain (1); Region (1) |
| Keywords | Cytoplasm;Methyltransferase;Reference proteome;S-adenosyl-L-methionine;Transferase;tRNA processing |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 28,287 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:36899 |
| Cross Reference Brenda |