| IED ID | IndEnz0005000867 |
| Enzyme Type ID | lipase000867 |
| Protein Name |
Salicylic acid-binding protein 2 NtSABP2 EC 3.1.1.- Methyl salicylate esterase |
| Gene Name | SABP2 LOC107791137 |
| Organism | Nicotiana tabacum (Common tobacco) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Nicotianoideae Nicotianeae Nicotiana Nicotiana tabacum (Common tobacco) |
| Enzyme Sequence | MKEGKHFVLVHGACHGGWSWYKLKPLLEAAGHKVTALDLAASGTDLRKIEELRTLYDYTLPLMELMESLSADEKVILVGHSLGGMNLGLAMEKYPQKIYAAVFLAAFMPDSVHNSSFVLEQYNERTPAENWLDTQFLPYGSPEEPLTSMFFGPKFLAHKLYQLCSPEDLALASSLVRPSSLFMEDLSKAKYFTDERFGSVKRVYIVCTEDKGIPEEFQRWQIDNIGVTEAIEIKGADHMAMLCEPQKLCASLLEIAHKYN |
| Enzyme Length | 260 |
| Uniprot Accession Number | Q6RYA0 |
| Absorption | |
| Active Site | ACT_SITE 81; /note="Acyl-ester intermediate"; /evidence="ECO:0000269|PubMed:15668381, ECO:0000269|PubMed:17916738"; ACT_SITE 210; /note="Charge relay system"; /evidence="ECO:0000269|PubMed:15668381"; ACT_SITE 238; /note="Charge relay system"; /evidence="ECO:0000269|PubMed:17916738" |
| Activity Regulation | ACTIVITY REGULATION: Esterase activity is down-regulated by salicylic acid (SA) or by tetraFA, a synthetic SA analog. {ECO:0000269|PubMed:15668381, ECO:0000269|PubMed:19131332}. |
| Binding Site | BINDING 13; /note="Salicylic acid; via amide nitrogen"; /evidence="ECO:0000269|PubMed:15668381, ECO:0007744|PDB:1Y7I"; BINDING 81; /note="Salicylic acid"; /evidence="ECO:0000269|PubMed:15668381, ECO:0007744|PDB:1Y7I"; BINDING 159; /note="Salicylic acid"; /evidence="ECO:0000269|PubMed:15668381, ECO:0007744|PDB:1Y7I"; BINDING 238; /note="Salicylic acid"; /evidence="ECO:0000269|PubMed:15668381, ECO:0007744|PDB:1Y7I"; BINDING 253; /note="Salicylic acid"; /evidence="ECO:0000269|PubMed:15668381, ECO:0007744|PDB:1Y7I"; BINDING 257; /note="Salicylic acid"; /evidence="ECO:0000269|PubMed:15668381, ECO:0007744|PDB:1Y7I" |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O + methyl salicylate = H(+) + methanol + salicylate; Xref=Rhea:RHEA:33611, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:30762, ChEBI:CHEBI:31832; Evidence={ECO:0000269|PubMed:15668381};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33612; Evidence={ECO:0000269|PubMed:15668381}; |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Required to convert methyl salicylate (MeSA) to salicylic acid (SA) as part of the signal transduction pathways that activate systemic acquired resistance in systemic tissue. MeSA is believed to be an inactive form that needs to be demethylated to exert a biological effect. Also able to catalyze the conversion of acibenzolar-S-methyl into acibenzolar to induce systemic acquired resistance. {ECO:0000269|PubMed:14673096, ECO:0000269|PubMed:15668381, ECO:0000269|PubMed:17916738, ECO:0000269|PubMed:19131332, ECO:0000269|PubMed:20621100, ECO:0000269|PubMed:20797615}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Plant hormone biosynthesis. {ECO:0000269|PubMed:15668381}. |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (11); Binding site (6); Chain (1); Helix (14); Mutagenesis (5); Turn (3) |
| Keywords | 3D-structure;Hydrolase;Immunity;Innate immunity;Plant defense;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (3) |
| Cross Reference PDB | 1XKL; 1Y7H; 1Y7I; |
| Mapped Pubmed ID | 26110207; 26934101; |
| Motif | |
| Gene Encoded By | |
| Mass | 29,304 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=8.6 uM for methyl salicylate (MeSA) {ECO:0000269|PubMed:15668381}; Note=kcat is 0.45 sec(-1) with methyl salicylate as substrate. {ECO:0000269|PubMed:15668381}; |
| Metal Binding | |
| Rhea ID | RHEA:33611; RHEA:33612 |
| Cross Reference Brenda |