IED ID | IndEnz0005000868 |
Enzyme Type ID | lipase000868 |
Protein Name |
Lipase EC 3.1.1.3 Triacylglycerol lipase |
Gene Name | LIP |
Organism | Thermomyces lanuginosus (Humicola lanuginosa) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Trichocomaceae Thermomyces Thermomyces lanuginosus (Humicola lanuginosa) |
Enzyme Sequence | MRSSLVLFFVSAWTALASPIRREVSQDLFNQFNLFAQYSAAAYCGKNNDAPAGTNITCTGNACPEVEKADATFLYSFEDSGVGDVTGFLALDNTNKLIVLSFRGSRSIENWIGNLNFDLKEINDICSGCRGHDGFTSSWRSVADTLRQKVEDAVREHPDYRVVFTGHSLGGALATVAGADLRGNGYDIDVFSYGAPRVGNRAFAEFLTVQTGGTLYRITHTNDIVPRLPPREFGYSHSSPEYWIKSGTLVPVTRNDIVKIEGIDATGGNNQPNIPDIPAHLWYFGLIGTCL |
Enzyme Length | 291 |
Uniprot Accession Number | O59952 |
Absorption | |
Active Site | ACT_SITE 168; /note=Nucleophile; /evidence=ECO:0000269|PubMed:8014587; ACT_SITE 223; /note=Charge relay system; /evidence=ECO:0000305|PubMed:8014587; ACT_SITE 280; /note=Charge relay system; /evidence=ECO:0000305|PubMed:8014587 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; |
DNA Binding | |
EC Number | 3.1.1.3 |
Enzyme Function | |
Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Active over a broad temperature range.; |
PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Active at alkaline pHs (up to pH 12 approximately).; |
Pathway | |
nucleotide Binding | |
Features | Active site (3); Beta strand (17); Chain (1); Disulfide bond (3); Helix (12); Mutagenesis (3); Propeptide (1); Signal peptide (1); Turn (3) |
Keywords | 3D-structure;Cleavage on pair of basic residues;Disulfide bond;Hydrolase;Lipid degradation;Lipid metabolism;Signal;Zymogen |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000255 |
Structure 3D | X-ray crystallography (27) |
Cross Reference PDB | 1DT3; 1DT5; 1DTE; 1DU4; 1EIN; 1GT6; 1TIB; 4DYH; 4EA6; 4FLF; 4GBG; 4GHW; 4GI1; 4GLB; 4GWL; 4KJX; 4N8S; 4S0X; 4ZGB; 5AP9; 6HW1; 6OR3; 6XOK; 6XRV; 6XS3; 7APN; 7APP; |
Mapped Pubmed ID | 11895431; 27693248; 31098026; 34602865; |
Motif | |
Gene Encoded By | |
Mass | 31,807 |
Kinetics | |
Metal Binding | |
Rhea ID | RHEA:12044 |
Cross Reference Brenda | 3.1.1.3; |