| IED ID | IndEnz0005000868 |
| Enzyme Type ID | lipase000868 |
| Protein Name |
Lipase EC 3.1.1.3 Triacylglycerol lipase |
| Gene Name | LIP |
| Organism | Thermomyces lanuginosus (Humicola lanuginosa) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Trichocomaceae Thermomyces Thermomyces lanuginosus (Humicola lanuginosa) |
| Enzyme Sequence | MRSSLVLFFVSAWTALASPIRREVSQDLFNQFNLFAQYSAAAYCGKNNDAPAGTNITCTGNACPEVEKADATFLYSFEDSGVGDVTGFLALDNTNKLIVLSFRGSRSIENWIGNLNFDLKEINDICSGCRGHDGFTSSWRSVADTLRQKVEDAVREHPDYRVVFTGHSLGGALATVAGADLRGNGYDIDVFSYGAPRVGNRAFAEFLTVQTGGTLYRITHTNDIVPRLPPREFGYSHSSPEYWIKSGTLVPVTRNDIVKIEGIDATGGNNQPNIPDIPAHLWYFGLIGTCL |
| Enzyme Length | 291 |
| Uniprot Accession Number | O59952 |
| Absorption | |
| Active Site | ACT_SITE 168; /note=Nucleophile; /evidence=ECO:0000269|PubMed:8014587; ACT_SITE 223; /note=Charge relay system; /evidence=ECO:0000305|PubMed:8014587; ACT_SITE 280; /note=Charge relay system; /evidence=ECO:0000305|PubMed:8014587 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; |
| DNA Binding | |
| EC Number | 3.1.1.3 |
| Enzyme Function | |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Active over a broad temperature range.; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Active at alkaline pHs (up to pH 12 approximately).; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (17); Chain (1); Disulfide bond (3); Helix (12); Mutagenesis (3); Propeptide (1); Signal peptide (1); Turn (3) |
| Keywords | 3D-structure;Cleavage on pair of basic residues;Disulfide bond;Hydrolase;Lipid degradation;Lipid metabolism;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (27) |
| Cross Reference PDB | 1DT3; 1DT5; 1DTE; 1DU4; 1EIN; 1GT6; 1TIB; 4DYH; 4EA6; 4FLF; 4GBG; 4GHW; 4GI1; 4GLB; 4GWL; 4KJX; 4N8S; 4S0X; 4ZGB; 5AP9; 6HW1; 6OR3; 6XOK; 6XRV; 6XS3; 7APN; 7APP; |
| Mapped Pubmed ID | 11895431; 27693248; 31098026; 34602865; |
| Motif | |
| Gene Encoded By | |
| Mass | 31,807 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:12044 |
| Cross Reference Brenda | 3.1.1.3; |