| IED ID | IndEnz0005000871 |
| Enzyme Type ID | lipase000871 |
| Protein Name |
Phospholipase A1 member A EC 3.1.1.- |
| Gene Name | pla1a zgc:77160 |
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio) |
| Enzyme Sequence | MAWKWIKAFVYLSVCVTSALGDDEEASASKCADFNNTTWLEYRQATKLQVQYLLLTRKNANCASLFTQDCLNHTQKHTAYFNSSLPTKVIVHGYRALGSKPSWVSGLAQALLREEDVNVLVVDWVYGASFAYNLVVENYKEVAVQISVLINQLTKYGSTLESFHFIGVSLGAHVSGFVGTLFHGKLGRITGLDPAGPMFKSADPFDRLDSSDALFVEAIHTDSDYFGISIPVGHVDFFLNGGMDQAGCARSRFASMYGYVICDHMRALHVYMSALNGSCPLIGFPCSGYEEFLAGKCITCDDPFNGTCPQIGLLKNSGITATPLPNQEKVYLLTTASGPFCAHHILVELNVSRLDKTAEVQLILKSIGHPETELNLKLYTDETRYKTVAAHPEQLCKIDSMQLINTGGRFYRQGDIHFEYICISEIPQTRGMDPLCVKNIHIGRGVPWSHDFVQVC |
| Enzyme Length | 456 |
| Uniprot Accession Number | Q6NYZ4 |
| Absorption | |
| Active Site | ACT_SITE 169; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 193; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 264; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=1-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + H2O = (9Z)-octadecenoate + H(+) + sn-glycero-3-phospho-L-serine; Xref=Rhea:RHEA:40499, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:64765, ChEBI:CHEBI:74617; Evidence={ECO:0000250|UniProtKB:P97535};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40500; Evidence={ECO:0000250|UniProtKB:P97535}; CATALYTIC ACTIVITY: Reaction=1,2-di-(9Z)-octadecenoyl-sn-glycero-3-phospho-L-serine + H2O = (9Z)-octadecenoate + 2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + H(+); Xref=Rhea:RHEA:40491, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:74905, ChEBI:CHEBI:77342; Evidence={ECO:0000250|UniProtKB:P97535};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40492; Evidence={ECO:0000250|UniProtKB:P97535}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phospho-L-serine + H2O = 2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phospho-L-serine + H(+) + hexadecanoate; Xref=Rhea:RHEA:41187, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:75032, ChEBI:CHEBI:77830; Evidence={ECO:0000250|UniProtKB:P97535};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41188; Evidence={ECO:0000250|UniProtKB:P97535}; |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Hydrolyzes the ester bond at the sn-1 position of glycerophospholipids and produces 2-acyl lysophospholipids. Hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1-acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) or phosphatidylinositol (PI). {ECO:0000250|UniProtKB:P97535}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (3); Glycosylation (7); Signal peptide (1) |
| Keywords | Disulfide bond;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 50,476 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:40499; RHEA:40500; RHEA:40491; RHEA:40492; RHEA:41187; RHEA:41188 |
| Cross Reference Brenda |