Detail Information for IndEnz0005000873
IED ID IndEnz0005000873
Enzyme Type ID lipase000873
Protein Name Sterol esterase 2
EC 3.1.1.13
Steryl ester hydrolase 2
Gene Name YEH2 YLR020C
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Enzyme Sequence MVNKVVDEVQRLVSAIILTSFMTGLFILSLWKNYVTVHFQHKNDPRDTRSSRTKIQPNDKKKKRPARHSRPLSISSTTPLDLQRDQENNIEYDRTVTSKLSMTSNASLSENGDGNANIKMETNVNQAPYAAENPFQNIALAEDTKLVPDLKYYYKEYGIDIEEFEVETDDGFIIDLWHFKSRLNDGVEEVKREPILLLHGLLQSCGAFASSGRKSLAYFLYESGFDVWLGNNRCGLNAKWNMKKLGNDHSKKWDWDMHQMVQYDLKALINYVLDSTGYAKLSLVAHSQGTTQGFMGLVNGEKLYASDFKLVDKLENFVALAPAVYPGPLLDEKAFVRLMAKGIDSPWYFGRRSFIPLMMTMRKLMVGTKIFSFLSYIMFNYLFDWNDVLWDRVLRDRNFLFSPVHISVKLMQWWLSPLPNKLSFKKGAEKIFPDKKTWFPIAKNDDDSGNNLDNNKLHLNPKRQNSEEFPHIIMFIPKQDRLVDGERLINHFINHEANAVYKIWYIDEYSHLDVLWAHDVIDRIGKPMIENLRFPNAR
Enzyme Length 538
Uniprot Accession Number Q07950
Absorption
Active Site ACT_SITE 287; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 480; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 511; /note=Charge relay system; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a sterol ester + H2O = a fatty acid + a sterol + H(+); Xref=Rhea:RHEA:10100, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15889, ChEBI:CHEBI:28868, ChEBI:CHEBI:35915; EC=3.1.1.13; Evidence={ECO:0000269|PubMed:15713625};
DNA Binding
EC Number 3.1.1.13
Enzyme Function FUNCTION: Mediates the hydrolysis of steryl esters. Required for mobilization of steryl ester, thereby playing a central role in lipid metabolism. {ECO:0000269|PubMed:15632184, ECO:0000269|PubMed:15713625}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Compositional bias (1); Modified residue (2); Region (1); Topological domain (2); Transmembrane (1)
Keywords Cell membrane;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Phosphoprotein;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15632184, ECO:0000269|PubMed:15713625}; Single-pass type II membrane protein {ECO:0000269|PubMed:15632184, ECO:0000269|PubMed:15713625}.
Modified Residue MOD_RES 73; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198"; MOD_RES 107; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:19779198"
Post Translational Modification PTM: Not glycosylated.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 14587103; 14690591; 16246075; 16835446; 16919863; 17434796; 18258205; 18269180; 18467557; 19111628; 19536198; 21621788; 22194828; 22345606; 23139841; 23202731; 23275493; 24520995; 24597968; 25720564; 25894691; 26162625; 26636650; 28772;
Motif
Gene Encoded By
Mass 62,447
Kinetics
Metal Binding
Rhea ID RHEA:10100
Cross Reference Brenda 3.1.1.13;