Detail Information for IndEnz0005000881
IED ID IndEnz0005000881
Enzyme Type ID lipase000881
Protein Name Neutral cholesterol ester hydrolase 1
NCEH
EC 3.1.1.-
Arylacetamide deacetylase-like 1
Gene Name Nceh1 Aadacl1
Organism Rattus norvegicus (Rat)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat)
Enzyme Sequence MRSSCVLLAALLALVAYYVYIPLPSAVSDPWKLMLLDATFRGAQQVSNLIHSLGLSHHLITLNFIIISFGKKSARSSPRVKVTDTDFDGVEVRVFEGPPKPDEPLRRSVVYIHGGGWALASAKISYYDQLCTAMAEELNAVIVSIEYRLVPQVYFPEQIHDVIRATKYFLQPEVLDKYKVDPGRVGVSGDSAGGNLAAALGQQFTYVESLKNKLKLQALIYPVLQALDFNTPSYQQSMNTPILPRHVMVRYWVDYFKGNYDFVEAMIVNNHTSLDVERAAALRARLDWTSLLPSSIKKNYKPVLQTIGDARIVKEIPQLLDAAASPLIAEQEVLQALPKTYILTCEHDVLRDDGIMYAKRLESAGVNVTLDHFEDGFHGCMIFTSWPTNFSVGIRTRDSYFKWLDQNL
Enzyme Length 408
Uniprot Accession Number B2GV54
Absorption
Active Site ACT_SITE 191; /evidence=ECO:0000255|PROSITE-ProRule:PRU10038; ACT_SITE 348; /evidence=ECO:0000255|PROSITE-ProRule:PRU10038; ACT_SITE 378; /evidence=ECO:0000255|PROSITE-ProRule:PRU10038
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=cholesteryl (9Z-octadecenoate) + H2O = (9Z)-octadecenoate + cholesterol + H(+); Xref=Rhea:RHEA:33875, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16113, ChEBI:CHEBI:30823, ChEBI:CHEBI:46898; Evidence={ECO:0000250|UniProtKB:Q8BLF1};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33876; Evidence={ECO:0000250|UniProtKB:Q8BLF1}; CATALYTIC ACTIVITY: Reaction=1-O-hexadecyl-2-acetyl-sn-glycerol + H2O = 1-O-hexadecyl-sn-glycerol + acetate + H(+); Xref=Rhea:RHEA:38563, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:34115, ChEBI:CHEBI:75936; Evidence={ECO:0000250|UniProtKB:Q8BLF1};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38564; Evidence={ECO:0000250|UniProtKB:Q8BLF1};
DNA Binding
EC Number 3.1.1.-
Enzyme Function FUNCTION: Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (By similarity). May be responsible for cholesterol ester hydrolysis in macrophages (By similarity). Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds (By similarity). {ECO:0000250|UniProtKB:Q8BLF1}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Glycosylation (3); Motif (1); Topological domain (2); Transmembrane (1)
Keywords Cell membrane;Endoplasmic reticulum;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Microsome;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q6PIU2}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:Q6PIU2}. Microsome {ECO:0000250|UniProtKB:Q8BLF1}.
Modified Residue
Post Translational Modification PTM: N-glycosylated. {ECO:0000250|UniProtKB:Q6PIU2}.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif MOTIF 113..115; /note=Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole; /evidence=ECO:0000250|UniProtKB:Q5NUF3
Gene Encoded By
Mass 45,822
Kinetics
Metal Binding
Rhea ID RHEA:33875; RHEA:33876; RHEA:38563; RHEA:38564
Cross Reference Brenda